Anti-TBL1XR1 Antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TBL1XR1 Antibody (AB327196)

Immunofluorescent staining of human cell line U-2 OS using ab327196 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBL1XR1 Antibody (AB327196)

Immunohistochemical staining of human cerebral cortex using ab327196 at 1 : 200–1 : 500 dilution, showing moderate to strong nuclear positivity in neurons.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBL1XR1 Antibody (AB327196)

Immunohistochemical staining of human skeletal muscle using ab327196 at 1 : 200–1 : 500 dilution, showing weak to moderate nuclear positivity in myocytes.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBL1XR1 Antibody (AB327196)

Immunohistochemical staining of human prostate using ab327196 at 1 : 200–1 : 500 dilution, showing moderate to strong nuclear positivity in glandular cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBL1XR1 Antibody (AB327196)

Immunohistochemical staining of human testis using ab327196 at 1 : 200–1 : 500 dilution, showing moderate to strong nuclear positivity in cells in seminiferous ducts.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBL1XR1 Antibody (AB327196)

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using ab327196 at 1 : 200–1 : 500 dilution. Corresponding TBL1XR1 RNA-seq data are presented for the same tissues.

• ChIP

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ChIP - Anti-TBL1XR1 Antibody (AB327196)

ChIP-Exo-Seq composite graph for ab327196 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

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Western blot - Anti-TBL1XR1 Antibody (AB327196)

Western blot analysis using ab327196 at 0.04–0.4 µg/ml.
Lane 1 : Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2 : Human cell line RT-4

All lanes:

Western blot - Anti-TBL1XR1 Antibody (ab327196)

All lanes:

Human cell line RT-4

Predicted band size: 55.6 kDa

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