AB117761

Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9]

Name: Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) | Abcam
Brand: Abcam Limited
SKU: AB117761
Price: 500 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal TBLR1/TBL1XR1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, African green monkey, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBL1XR1.

View Alternative Names

IRA1, TBLR1, TBL1XR1, F-box-like/WD repeat-containing protein TBL1XR1, Nuclear receptor corepressor/HDAC3 complex subunit TBLR1, TBL1-related protein 1, Transducin beta-like 1X-related protein 1

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

ab117761, at 1/150 dilution, staining TBLR1/TBL1XR1 in HeLa cells by Immunocytochemistry. This image was produced with antibody produced in ascites.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

Immunocytochemical staining of HeLa cells using anti-TBLR1/TBL1XR1 antibody (ab117761) at a dilution of 1 : 200. Cells were fixed with 4% paraformaldehyde. This image was produced with antibody produced in ascites.

Unknown

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

All lanes:

K562 whole cell lysate at 40 µg

Predicted band size: 56 kDa

Observed band size: 60 kDa

false

Unknown

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 40 µg

Lane 2:

Mouse brain lysate at 40 µg

Predicted band size: 56 kDa

Observed band size: 60 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

ab117761, at 1/500 - 1/1000 dilution, staining TBLR1/TBL1XR1 in paraffin-embedded Human breast cancer by Imunohistochemistry. This image was produced with antibody produced in ascites.

Supplier Data

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

All lanes:

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

Lane 2:

CEM cell lysate

Lane 3:

K562 cell lysate

Lane 4:

HeLa cell lysate

Predicted band size: 56 kDa

false

Unknown

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

Lane 1:

Mouse Brain Lysate

Lane 2:

Mouse Heart Lysate

Lane 3:

K562 cell Lysate

Predicted band size: 56 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4F3-A8-D9

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey, Mouse

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TBL1XR1. The exact immunogen used to generate this antibody is proprietary information.

Q9BZK7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification technique

Cross-flow filtration

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBLR1 also known as TBL1XR1 is a protein that plays an important role in transcription regulation mechanisms. This protein with a molecular mass of approximately 64 kDa is expressed in various tissues including the brain and lungs. It functions as an adaptor protein facilitating interactions between different transcription factors and the corepressor complex. By doing so it enables the repression or activation of specific genes according to cellular needs.

Biological function summary

TBLR1 participates in the regulation of gene expression by being part of the SMRT/N-CoR repressor complex. This complex interacts with nuclear receptors to modulate gene transcription. Through this function TBLR1 contributes to processes involved in maintaining cellular homeostasis. Its role in this complex marks it as a contributor to the fine-tuning of cellular responses to external stimuli which is essential for normal cellular function.

Pathways

The regulation of transcription by TBLR1 integrates into significant signaling pathways like Wnt and nuclear receptor pathways. It partners with proteins such as beta-catenin in the Wnt signaling pathway and corepressors in the nuclear receptor pathway. Through these partnerships TBLR1 affects important developmental and metabolic processes linking transcriptional machinery to broader regulatory networks in the cell.

TBLR1 has associations with conditions such as cancer and autism spectrum disorder. Mutations or dysregulation in the TBLR1 gene can affect normal cellular signaling leading to aberrant cell growth in cancers or disrupted neural development in autism. Additionally it is connected to the action of the protein N-CoR which is also implicated in these diseases. Through these associations TBLR1 becomes a target of interest for understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation.

See full target information TBL1XR1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine 241:239-247 PubMed28344213

2017

Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Ma,Nina Yu

Oncogene 36:1873-1886 PubMed27694893

2016

Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.

Applications

IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Q Zhou,X Wang,Z Yu,X Wu,X Chen,J Li,Z Zhu,B Liu,L Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9]

Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (AB117761)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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