Skip to main content

Rabbit Recombinant Monoclonal TBLR1/TBL1XR1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Recombinant full length protein - Human, Mouse samples. Cited in 2 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (AB190796), expandable thumbnail
  • Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (AB190796), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (AB190796), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (AB190796), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (AB190796), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Tested
Expected
Expected
Recombinant full length protein - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Recombinant full length protein - Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation.

Additional Targets

TBL1Y, TBL1X

Alternative names

IRA1, TBLR1, TBL1XR1, F-box-like/WD repeat-containing protein TBL1XR1, Nuclear receptor corepressor/HDAC3 complex subunit TBLR1, TBL1-related protein 1, Transducin beta-like 1X-related protein 1

Recommended products

Rabbit Recombinant Monoclonal TBLR1/TBL1XR1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Recombinant full length protein - Human, Mouse samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16153
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TBL1 also known as TBLR1/TBL1XR1 and TBL1Y are part of the WD40 repeat-containing proteins each with a mass of approximately 55 kDa. These proteins play a role in the mechanics of transcription regulation and signal transduction. TBL1 and its variants are expressed in various tissues including the brain and liver suggesting a broad functional range. They interact with other protein complexes to modulate the chromatin structure and influence gene expression.

Biological function summary

TBL1 and its related proteins function as integral components of the nuclear receptor corepressor complex. They participate alongside other corepressor proteins to mediate transcriptional silencing. TBL1 proteins recruit enzymatic partners to specific genomic locations facilitating targeted gene repression and contributing to processes like metabolic regulation and circadian rhythm maintenance.

Pathways

TBL1 is significantly involved in the Wnt signaling and NF-kB pathways. In the Wnt pathway it interacts with proteins like beta-catenin to influence cell fate decisions. In the NF-kB pathway TBL1 affects the transcriptional regulation of genes involved in immune and inflammatory responses with targets that include RELA and NFKB1.

Associated diseases and disorders

TBL1 proteins are associated with conditions such as deafness and cancer. Mutations or dysregulation of TBL1 can lead to sensorineural deafness likely due to its role in cochlear function and maintenance. Moreover TBL1 influences tumor progression by interacting with oncogenic proteins like beta-catenin contributing to cancerous transformations. Understanding these relationships highlights the importance of TBL1 in maintaining cellular homeostasis and its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796), expandable thumbnail

    Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796)

    All lanes: Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796) at 1/1000 dilution

    Lane 1: K562 cell lysate at 20 µg

    Lane 2: Hela cell lysate at 20 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

  • Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796), expandable thumbnail

    Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796)

    All lanes: Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796) at 1/1000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: Raw 264.7 cell lysate at 10 µg

    Lane 3: PC-12 cell lysate at 10 µg

    Lane 4: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostatic hyperplasia tissue, labeling TBLR1/TBL1XR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse muscle tissue, labeling TBLR1/TBL1XR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796)

    Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde, labeling TBLR1/TBL1XR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as the secondary antibody at 1/200. Counterstained with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796)

    Immunofluorescence analysis of PC-3 cells fixed in 4% paraformaldehyde, labeling TBLR1/TBL1XR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as a secondary antibody at 1/200. Counterstained with DAPI.

  • Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796), expandable thumbnail

    Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796)

    All lanes: Western blot - Anti-TBLR1/TBL1XR1+TBL1X+TBL1Y antibody [EPR16153] (ab190796) at 1/5000 dilution

    Lane 1: His-tagged human full length TBL1Y protein at 10 ng with 5% NFDM/TBST

    Lane 2: His-tagged human full length TBL1X protein at 10 ng with 5% NFDM/TBST

    Lane 3: His-tagged human full length TBL1R protein at 10 ng with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50-60 kDa

    Exposure time: 120s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com