Anti-TBP-1 antibody [EPR12152] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12152] - BSA and Azide free (AB249681)

This data was developed using ab171969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling TBP-1 using ab171969 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TBP-1 antibody [EPR12152] - BSA and Azide free (AB249681)

This data was developed using ab171969, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293 cells, labeling TBP-1 using ab171969 at a 1/10 dilution (red) or a rabbit IgG negative control (green).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TBP-1 antibody [EPR12152] - BSA and Azide free (AB249681)

This data was developed using ab171969, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of 293 cells labeling TBP-1 using ab171969 at a 1/250 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12152] - BSA and Azide free (AB249681)

This data was developed using ab171969, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling TBP-1 using ab171969 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-TBP-1 antibody [EPR12152] - BSA and Azide free (AB249681)

This data was developed using ab171969, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (Lane 1) HeLa cell lysate or (Lane 2) 1X PBS (negative control) using ab171969 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-TBP-1 antibody [EPR12152] - N-terminal (<a href='/en-us/products/primary-antibodies/tbp-1-antibody-epr12152-n-terminal-ab171969'>ab171969</a>)

Predicted band size: 49 kDa

false

• WB

Supplier Data

Western blot - Anti-TBP-1 antibody [EPR12152] - BSA and Azide free (AB249681)

This data was developed using ab171969, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TBP-1 antibody [EPR12152] - N-terminal (<a href='/en-us/products/primary-antibodies/tbp-1-antibody-epr12152-n-terminal-ab171969'>ab171969</a>) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 49 kDa

true