AB171974

Anti-TBP-1 antibody [EPR12153]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal TBP-1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

TBP1, PSMC3, 26S proteasome regulatory subunit 6A, 26S proteasome AAA-ATPase subunit RPT5, Proteasome 26S subunit ATPase 3, Proteasome subunit P50, Tat-binding protein 1, TBP-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (AB171974)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (AB171974)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TBP-1 with ab171974 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (AB171974)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TBP-1 with ab171974 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-TBP-1 antibody [EPR12153] (AB171974)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TBP-1 antibody [EPR12153] (AB171974)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling TBP-1 with ab171974 at 1/100 dilution (red) or a Rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-TBP-1 antibody [EPR12153] (AB171974)

All lanes:

Western blot - Anti-TBP-1 antibody [EPR12153] (ab171974) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Predicted band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12153

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBP-1 also called Tat-binding protein-1 is a critical player in the regulation of protein degradation. It is a component of the 19S regulatory particle of the 26S proteasome. The molecular mass of TBP-1 is approximately 56 kDa. Expression of TBP-1 is noted in various tissues reflecting its essential role in cellular processes. As a proteasome member it contributes to the recognition and unfolding of ubiquitinated proteins designated for degradation.

Biological function summary

TBP-1 functions as an ATPase in the 26S proteasome complex aiding in the breakdown of proteins. This breakdown is important for maintaining protein homeostasis by removing misfolded or damaged proteins. In the proteasome complex TBP-1 collaborates with other ATPases to control access of substrates to the proteolytic core. This role ensures a regulated protein turnover which is important for cell cycle progression and stress responses.

Pathways

TBP-1 serves a significant role in the ubiquitin-proteasome pathway and the regulation of transcription. In the ubiquitin-proteasome pathway it collaborates with other regulatory subunits to modulate proteasomal activity. TBP-1 is associated with proteins like Rpt5 and Rpt1 which are also ATPases in the 19S regulatory particle coordinating the denaturation and translocation of proteins into the 20S core particle for degradation.

TBP-1's malfunction or altered expression can contribute to neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. In these conditions improper protein degradation leads to accumulation of toxic protein aggregates. Through these pathways TBP-1 interacts with proteins like alpha-synuclein in Parkinson's disease where its dysfunction exacerbates aggregation. Understanding TBP-1's role offers insights for therapeutic approaches aimed at modulating the proteasomal degradation machinery in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC3 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

See full target information PSMC3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 34:e22454 PubMed31981282

2020

ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR-8/SVneo cells via modulating the epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohan Zou,Ruirui Dong,Ping Zou,Xina Meng,Ting Zhang,Liang Luo,Na Li,Yao Wang,Jing Wang,Tiejun Wang,Yan Zhang,Minjian Chen,Conghua Zhou,Fei Han

Frontiers in neuroscience 12:301 PubMed29770111

2018

Chronic Caffeine Treatment Protects Against α-Synucleinopathy by Reestablishing Autophagy Activity in the Mouse Striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Luan,Xiangpeng Ren,Wu Zheng,Zhenhai Zeng,Yingzi Guo,Zhidong Hou,Wei Guo,Xingjun Chen,Fei Li,Jiang-Fan Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TBP-1 antibody [EPR12153]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (AB171974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12153] (AB171974)

Flow Cytometry (Intracellular) - Anti-TBP-1 antibody [EPR12153] (AB171974)

Western blot - Anti-TBP-1 antibody [EPR12153] (AB171974)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise