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AB139184

Anti-Tbp7 antibody [EPR9911(B)]

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(3 Publications)

Rabbit Recombinant Monoclonal Tbp7 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

MIP224, TBP7, PSMC4, 26S proteasome regulatory subunit 6B, 26S proteasome AAA-ATPase subunit RPT3, MB67-interacting protein, Proteasome 26S subunit ATPase 4, Tat-binding protein 7, TBP-7

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Tbp7 antibody [EPR9911(B)] (AB139184)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tbp7 antibody [EPR9911(B)] (AB139184)

Immunofluorescent analysis of U87-MG cells labelling Tpb7 with ab139184 at 1/100 dilution.

Western blot - Anti-Tbp7 antibody [EPR9911(B)] (AB139184)
  • WB

Unknown

Western blot - Anti-Tbp7 antibody [EPR9911(B)] (AB139184)

All lanes:

Western blot - Anti-Tbp7 antibody [EPR9911(B)] (ab139184) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9911(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tbp7 also known as Tat-binding protein 7 is an essential component linked to various cellular processes. It has a molecular mass of about 76 kDa and is found in many tissues with significant expression in the brain and immune cells. It operates mechanically by interacting with components of the cellular machinery to influence transcription regulation and growth signaling. Tbp7's involvement in these processes highlights its importance in maintaining cellular homeostasis.
Biological function summary

Tbp7 plays an important role in the ubiquitin-proteasome system contributing to protein degradation and turnover. It forms a part of the ATP-dependent proteasome activator complex where it collaborates with other proteins like PSMC components to regulate protein breakdown. This system ensures the removal of damaged or unneeded proteins thereby protecting cells from stress and maintaining protein quality control.

Pathways

Tbp7 directly influences pathways such as the NF-kB signaling pathway and the mTOR pathway. It interacts with proteins like IkB and mTOR which are pivotal for controlling inflammation and autophagy respectively. By modulating these pathways Tbp7 helps balance cellular responses to stress and nutrient conditions reflecting its role as a regulator in cell survival and growth.

Researchers link Tbp7 to neurodegenerative diseases and certain immune disorders. Alterations in Tbp7 function can disrupt protein degradation exacerbating conditions like Alzheimer's disease and lupus. Proteins such as beta-amyloid in Alzheimer’s and immune cell activators in lupus are affected through Tbp7’s regulation of protein decay pathways highlighting its contribution to disease pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC4 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
See full target information PSMC4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 27:2437-2447 PubMed37436074

2023

PSMC4 promotes prostate carcinoma progression by regulating the CBX3-EGFR-PI3K-AKT-mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kaifeng Liu,Shengmin Zhang,Yongzhan Gong,Panyan Zhu,Weigan Shen,Qi Zhang

Nature immunology 22:1440-1451 PubMed34686860

2021

Mitochondrial complex II in intestinal epithelial cells regulates T cell-mediated immunopathology.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Fujiwara,Keisuke Seike,Michael D Brooks,Anna V Mathew,Ilya Kovalenko,Anupama Pal,Ho-Joon Lee,Daniel Peltier,Stephanie Kim,Chen Liu,Katherine Oravecz-Wilson,Lu Li,Yaping Sun,Jaeman Byun,Yoshinobu Maeda,Max S Wicha,Thomas L Saunders,Alnawaz Rehemtulla,Costas A Lyssiotis,Subramaniam Pennathur,Pavan Reddy

Molecular cell 71:1079-1091.e9 PubMed30146318

2018

Single Live Cell Monitoring of Protein Turnover Reveals Intercellular Variability and Cell-Cycle Dependence of Degradation Rates.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Brigitta Alber,Eric Raphael Paquet,Martina Biserni,Felix Naef,David Michael Suter
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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