Anti-TBR1 antibody ab31940 is a rabbit polyclonal antibody that is used in TBR1 western blotting and IHC. Suitable for human, mouse and rat samples.
- Tried and trusted by researchers since 2006
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Expected |
Mouse | Tested | Tested |
Rat | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Transcriptional repressor involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection (PubMed:25232744, PubMed:30250039). As transcriptional repressor of FEZF2, it blocks the formation of the corticospinal (CS) tract from layer 6 projection neurons, thereby restricting the origin of CS axons specifically to layer 5 neurons (By similarity).
T-box brain protein 1, T-brain-1, TBR-1, TES-56, TBR1
Anti-TBR1 antibody ab31940 is a rabbit polyclonal antibody that is used in TBR1 western blotting and IHC. Suitable for human, mouse and rat samples.
- Tried and trusted by researchers since 2006
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
TBR1 also known as T-Box Brain 1 is a transcription factor important for neuronal development. It weighs about 74 kDa. This protein is strongly expressed in the cerebral cortex during prenatal stages where it plays a role in regulating gene expression. TBR1 influences the differentiation and migration of cortical neurons which is critical for proper brain development.
TBR1 influences neuronal differentiation through its function as a transcription factor. It binds to specific DNA sequences to regulate gene expression required for neuronal identity and function. TBR1 is part of a larger transcriptional complex that includes other proteins like CASK and TBR2 contributing to the maturation of cortical neurons. TBR1's role in these processes highlights its significance in neuronal circuit formation in the cerebral cortex.
TBR1 is involved in the Wnt signaling and the reelin pathway which are both essential for normal brain development. In the Wnt signaling pathway TBR1 interacts with proteins such as LEF1 to influence cell fate decision and pattern formation. In the reelin pathway TBR1 work along with proteins like Reelin itself to regulate neuronal positioning promoting the correct layering of the cortex.
TBR1 has strong associations with autism spectrum disorders and intellectual disabilities. Mutations in TBR1 disrupt its role in gene regulation during brain development leading to the aforementioned neurological conditions. TBR1 also interacts with FOXP2 a protein involved in language and speech linking it further to communication-related aspects of these disorders.
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Terms & Conditions.
Gel type: MOPS
Blocking buffer: 3% milk block
All lanes: Western blot - Anti-TBR1 antibody (ab31940) at 1 µg/mL
Lane 1: Mouse hippocampus tissue lysate at 20 µg
Lane 2: Rat hippocampus tissue lysate at 20 µg
Lane 3: Human hippocampus tissue lysate at 20 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 4min
IHC image of TBR1 staining in a section of formalin-fixed paraffin-embedded normal E17 mouse brain performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab31940, 1/2000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab31940 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-TBR1 antibody (ab31940) at 1 µg/mL
Lane 1: Mouse hippocampus whole cell lysate at 20 µg
Lane 2: Rat hippocampus whole cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 50 kDa, 74 kDa
Exposure time: 4min
IHC-P image of TBR1 staining on mouse brain sections using ab31940 at a 1:200 dilution.
The sections were deparaffinized and subjected to heat mediated antigen retrieval. The sections were blocked using 7.5% goat serum for 2 hours at room temperature. ab31940 was diluted 1:200 using blocking buffer and incubated with the sections for 16 hours at 4°C. The secondary antibody used was Goat polyclonal to anti-rabbit conjugated to Alexa Fluor® 594 (1:400).
DAPI was used to counterstain nuclei.
(Image courtesy of Human Protein Atlas)
ab31940 staining TBR1 protein in normal human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab31940 at a 1/25 dilution for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
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