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Anti-TBR1 antibody ab31940 is a rabbit polyclonal antibody that is used in TBR1 western blotting and IHC. Suitable for human, mouse and rat samples.

- Tried and trusted by researchers since 2006


Images

Western blot - Anti-TBR1 antibody (AB31940), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (AB31940), expandable thumbnail
  • Western blot - Anti-TBR1 antibody (AB31940), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (AB31940), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (AB31940), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Expected
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Species

Rat

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcriptional repressor involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection (PubMed:25232744, PubMed:30250039). As transcriptional repressor of FEZF2, it blocks the formation of the corticospinal (CS) tract from layer 6 projection neurons, thereby restricting the origin of CS axons specifically to layer 5 neurons (By similarity).

Alternative names

Recommended products

Anti-TBR1 antibody ab31940 is a rabbit polyclonal antibody that is used in TBR1 western blotting and IHC. Suitable for human, mouse and rat samples.

- Tried and trusted by researchers since 2006

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TBR1 also known as T-Box Brain 1 is a transcription factor important for neuronal development. It weighs about 74 kDa. This protein is strongly expressed in the cerebral cortex during prenatal stages where it plays a role in regulating gene expression. TBR1 influences the differentiation and migration of cortical neurons which is critical for proper brain development.

Biological function summary

TBR1 influences neuronal differentiation through its function as a transcription factor. It binds to specific DNA sequences to regulate gene expression required for neuronal identity and function. TBR1 is part of a larger transcriptional complex that includes other proteins like CASK and TBR2 contributing to the maturation of cortical neurons. TBR1's role in these processes highlights its significance in neuronal circuit formation in the cerebral cortex.

Pathways

TBR1 is involved in the Wnt signaling and the reelin pathway which are both essential for normal brain development. In the Wnt signaling pathway TBR1 interacts with proteins such as LEF1 to influence cell fate decision and pattern formation. In the reelin pathway TBR1 work along with proteins like Reelin itself to regulate neuronal positioning promoting the correct layering of the cortex.

Associated diseases and disorders

TBR1 has strong associations with autism spectrum disorders and intellectual disabilities. Mutations in TBR1 disrupt its role in gene regulation during brain development leading to the aforementioned neurological conditions. TBR1 also interacts with FOXP2 a protein involved in language and speech linking it further to communication-related aspects of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Western blot - Anti-TBR1 antibody (ab31940), expandable thumbnail

    Western blot - Anti-TBR1 antibody (ab31940)

    Gel type: MOPS

    Blocking buffer: 3% milk block

    All lanes: Western blot - Anti-TBR1 antibody (ab31940) at 1 µg/mL

    Lane 1: Mouse hippocampus tissue lysate at 20 µg

    Lane 2: Rat hippocampus tissue lysate at 20 µg

    Lane 3: Human hippocampus tissue lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 74 kDa

    Observed band size: 74 kDa

    Exposure time: 4min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (ab31940), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (ab31940)

    IHC image of TBR1 staining in a section of formalin-fixed paraffin-embedded normal E17 mouse brain performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab31940, 1/2000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

  • Western blot - Anti-TBR1 antibody (ab31940), expandable thumbnail

    Western blot - Anti-TBR1 antibody (ab31940)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab31940 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-TBR1 antibody (ab31940) at 1 µg/mL

    Lane 1: Mouse hippocampus whole cell lysate at 20 µg

    Lane 2: Rat hippocampus whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa

    Observed band size: 50 kDa, 74 kDa

    Exposure time: 4min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (ab31940), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (ab31940)

    IHC-P image of TBR1 staining on mouse brain sections using ab31940 at a 1:200 dilution.

    The sections were deparaffinized and subjected to heat mediated antigen retrieval. The sections were blocked using 7.5% goat serum for 2 hours at room temperature. ab31940 was diluted 1:200 using blocking buffer and incubated with the sections for 16 hours at 4°C. The secondary antibody used was Goat polyclonal to anti-rabbit conjugated to Alexa Fluor® 594 (1:400).

    DAPI was used to counterstain nuclei.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (ab31940), expandable thumbnail
    Image courtesy of Human Protein Atlas

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (ab31940)

    (Image courtesy of Human Protein Atlas)
    ab31940 staining TBR1 protein in normal human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab31940 at a 1/25 dilution for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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