Anti-TBR1 antibody [EPR8138(2)]

9 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling tbr1 with ab183032 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 antibody [HM-2] at 1/100 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594 at 1/1000 (2μg/ml) was used as a MAP2 counterstain. The Nuclear counterstain was DAPI (Blue).

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  Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  ab183032 staining TBR-1 in Rat E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of rat E14 [PMID: 27848932].

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  Immunohistochemistry of paraffin embedded Rat E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml. Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of rat E14 [PMID: 27848932].

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  Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  The blocking and dilution buffer used was 5% NFDM/TBST

  All lanes: Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 0.9 µg/mL

  All lanes: Human brain lysate at 10 µg

  Secondary

  All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/100000 dilution

  Predicted band size: 74 kDa

  Exposure time: 30s

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  Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  ab183032 staining TBR-1 in Mouse E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of mouse E14 [PMID: 27848932].

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  Immunohistochemistry of paraffin embedded Mouse E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml. Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of mouse E14 [PMID: 27848932].

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  This image is courtesy of a customer review submitted by Daniel Berg.

  Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  ab183032 staining TBR1 in Human cerebral organoid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeabilized with 0.1% Triton X, blocked with 5% serum for 1 hour at room temperature and antigen retrieval was by heat mediation in Dako target retrieval. The sample was incubated with primary antibody (1:300 dilution) at 4^oC for 12 hours. An Alexa Fluor® 488 conjugate goat polyclonal (1/200) was used as the secondary antibody.

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  Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  The blocking and dilution buffer used was 5% NFDM/TBST.
  

  Exposure time
  Lane 1 and 2: 10 seconds;
  Lane 3: 5 seconds;
  Lane 4: 2 seconds;
  Lane 5: 3 minutes;
  Lane 6: 15 seconds

  All lanes: Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 0.9 µg/mL

  Lane 1: Mouse P1 Cerebral cortex lysate. at 20 µg

  Lane 2: Mouse hippocampus lysate at 20 µg

  Lane 3: E18 rat brain lysate at 20 µg

  Lane 4: Rat P5 cerebral cortex lysate at 20 µg

  Lane 5: Mouse brain lysate at 20 µg

  Lane 6: Rat brain lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 74 kDa

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  Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

  All lanes: Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 1/10000 dilution

  All lanes: Human fetal brain lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

  Predicted band size: 74 kDa