Anti-TBR1 antibody [EPR8138(2)]

• IHC-Fr

AbReview60326****

Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

ab183032 staining TBR1 in Human cerebral organoid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeabilized with 0.1% Triton X, blocked with 5% serum for 1 hour at room temperature and antigen retrieval was by heat mediation in Dako target retrieval. The sample was incubated with primary antibody (1 : 300 dilution) at 4^oC for 12 hours. An Alexa Fluor® 488 conjugate goat polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Daniel Berg.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

Immunohistochemistry of paraffin embedded Mouse E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml. Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of mouse E14 [PMID : 27848932].

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling tbr1 with ab183032 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 antibody [HM-2] at 1/100 dilution and ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594 at 1/1000 (2μg/ml) was used as a MAP2 counterstain. The Nuclear counterstain was DAPI (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

Immunohistochemistry of paraffin embedded Rat E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml. Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of rat E14 [PMID : 27848932].

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

ab183032 staining TBR-1 in Rat E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of rat E14 [PMID : 27848932].

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

ab183032 staining TBR-1 in Mouse E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of mouse E14 [PMID : 27848932].

• WB

Lab

Western blot - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

The blocking and dilution buffer used was 5% NFDM/TBST

All lanes:

Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 0.9 µg/mL

All lanes:

Human brain lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Predicted band size: 74 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

All lanes:

Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 1/10000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 74 kDa

false

• WB

Lab

Western blot - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

The blocking and dilution buffer used was 5% NFDM/TBST.

Exposure timeLane 1 and 2 : 10 seconds;
Lane 3 : 5 seconds;
Lane 4 : 2 seconds;
Lane 5 : 3 minutes;
Lane 6 : 15 seconds

All lanes:

Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 0.9 µg/mL

Lane 1:

Mouse P1 Cerebral cortex lysate. at 20 µg

Lane 2:

Mouse hippocampus lysate at 20 µg

Lane 3:

E18 rat brain lysate at 20 µg

Lane 4:

Rat P5 cerebral cortex lysate at 20 µg

Lane 5:

Mouse brain lysate at 20 µg

Lane 6:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 74 kDa

false