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Anti-TBR2 / Eomes antibody ab23345 is a rabbit polyclonal antibody that is used in TBR2 / Eomes western blotting and IHC. Suitable for human and mouse samples.

- Tried and trusted by researchers since 2006


Images

Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (AB23345), expandable thumbnail
  • Western blot - Anti-TBR2 / Eomes antibody (AB23345), expandable thumbnail
  • Western blot - Anti-TBR2 / Eomes antibody (AB23345), expandable thumbnail
  • Western blot - Anti-TBR2 / Eomes antibody (AB23345), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (AB23345), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-Fr
Human
Tested
Expected
Mouse
Expected
Tested
Rat
Predicted
Predicted
Common marmoset
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

0.4-2.5 µg/mL

Notes

Abcam recommends using milk as the blocking agent.

In our hands, when tested in western blot, this product typically gives a weaker signal in mouse tissue lysates compared to human cell lines. However, this product gives clean, specific staining in IHC-Fr on mouse E14.5 cortex. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/500

Notes

Abcam recommends the following antigen retrieval method: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Common marmoset

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.

Alternative names

Recommended products

Anti-TBR2 / Eomes antibody ab23345 is a rabbit polyclonal antibody that is used in TBR2 / Eomes western blotting and IHC. Suitable for human and mouse samples.

- Tried and trusted by researchers since 2006

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Tbr2 expression is observed in neuron progenitor compartments in development (the subventricular zone and ventricular zone) and expression rises and falls with cortical plate neurogenesis. Transition from radial glia to intermediate progenitor cell is associated with upregulation of Tbr2.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TBR2 also known as Eomes or Eomesodermin is a transcription factor with a molecular mass of approximately 72 kDa. It is a member of the T-box family and is expressed predominantly in the developing central nervous system especially in the intermediate progenitor cells in the subventricular zone of the cortex. TBR2 plays a significant role in neurogenesis by regulating the proliferation and differentiation of neuronal progenitor cells. This regulation ensures proper development of specific neural circuits critical for overall brain function.

Biological function summary

TBR2 is important in controlling the transition of neural progenitors during cortical development. It serves as an important marker for intermediate neuronal precursors highlighting its importance in the progression from radial glia to neurons. TBR2 does not function in a complex but works closely with the transcription factor Neurogenin 2 to control neuronal differentiation. Its activity determines the timing of neuronal differentiation making it indispensable for orderly cortical layer formation.

Pathways

TBR2 is involved in the Wnt signaling and Notch signaling pathways both key regulators of neurodevelopment. In the Wnt pathway TBR2 influences the balance between progenitor cell proliferation and differentiation by interacting with proteins such as Beta-catenin. In the Notch signaling pathway TBR2 modulates neural precursor cell fate decisions often in relation with proteins like DLL1. These pathways ensure proper brain structure and function by coordinating neural progenitor activity.

Associated diseases and disorders

TBR2 mutations or misregulation can lead to neurodevelopmental disorders such as microcephaly and intellectual disabilities. The protein's association with these conditions reflects its critical role in cortical development and neuronal differentiation. TBR2's dysregulation is often observed alongside the malfunction of its pathway partners like Beta-catenin in the development of such disorders illustrating the intricate interplay of genetic and molecular factors underlying these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail
    Image from Azim K et al., PLoS One. 2012;7(11):e49087. Fig 5.; doi: 10.1371/journal.pone.0049087. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)

    Dlx2 and Tbr2 identify non overlapping progenitor lineages in the adult mouse SVZ (subventricular zone).

    (A–B) Adult C57/BL6 mouse forebrain coronal sections at rostro-caudal point 1.2 relative to the bregma were immunostained for Dcx (red), Dlx2 or Tbr2 (green) and Ki67 (blue). Both progenitor populations show characteristics of migrating neuroblasts, as indicated by their Dcx expression (C–D). Adult Mash1 mouse forebrain coronal sections at rostro-caudal point 1.2 relative to the bregma were immunostained for Tbr2 (red) and Dlx2 (blue). Both Tbr2 and Dlx2 exhibited EGFP expression, but showed no colocalisation. Right side captions show cropped individual channels and the merges. Full panel insets are zoomed and cropped DAB stained photomicrographs of rostral periventricular sections for Tbr2 in the dorsal SVZ (A), Dlx2 in the dorso-lateral SVZ (B) and Mash1 in the ventro-lateral SVZ (C). Yellow arrows and arrowheads show respectively positive stained cell and low level TF staining. Dotted lines mark approximate boundaries of ventricular space. Flattened confocal z-stacks are of 14–15 μm thickness, including captions. Scale bars: 15 μm in full panels, 20 μm in captions and 25 μm in insets.

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail

    Western blot - Anti-TBR2 / Eomes antibody (ab23345)

    Gel type: MOPS

    Blocking buffer: 1% milk

    All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/mL

    Lane 1: Human PTA-6967 Whole Cell Lysate at 20 µg

    Lane 2: E14 Mouse Embryo Brain Tissue Lysate at 40 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 72 kDa

    Observed band size: 75 kDa, 85 kDa

    Exposure time: 12min

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail

    Western blot - Anti-TBR2 / Eomes antibody (ab23345)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab23345 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    Abcam recommends using milk as the blocking agent.

    All lanes: ab23345 at 1 mg/mL

    All lanes: Human ES Cells treated with Retinoic Acid (48h) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa

    Observed band size: 50 kDa, 65 kDa, 75 kDa, 85 kDa

    Exposure time: 20min

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail

    Western blot - Anti-TBR2 / Eomes antibody (ab23345)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab23345 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    Abcam recommends using milk as the blocking agent.

    All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/mL

    All lanes: Human ES Cells treated with Retinoic Acid (24h) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa

    Observed band size: 75 kDa, 85 kDa

    Exposure time: 20min

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)

    IHC image of TRB2 staining in a mouse brain E14 frozen tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.2% (v/v) Triton X-100 for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.05% (v/v) Triton X-100 and 1% (w/v) BSA with ab23345 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used to detect the primary antibody. The section was mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail

    Western blot - Anti-TBR2 / Eomes antibody (ab23345)

    All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/mL

    Lane 1: Human Mesendoderm (Day 2) Whole Cell Lysate at 10 µg

    Lane 2: E14 Mouse Embryo Brain Tissue Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 72 kDa

    Observed band size: 75 kDa, 85 kDa

    Exposure time: 4min

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Guillermo Estivill-Torrus

    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)

    ab23345 staining mouse developing cerebral cortex tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TX-100 prior to blocking with 2.5% serum for 1 hour at RT. The primary antibody was diluted 1/500 and incubated with the sample for 18 hours. A biotinylated pig anti-rabbit IgG antibody, diluted 1/500, was used as the secondary.

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail
    This image is a courtesy of Anonymous customer review

    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody (ab23345)

    ab23345 staining TBR2 / Eomes in mouse embryonic brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 1% BSA and normal Goat serum for 30 minutes at RT. The sample was incubated with primary antibody (1/1000 in TBS + BSA 1%) for 10 hours at 40C. An Alexa Fluor® 555-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/800 dilution.

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail
    This image was submitted as part of a review published on 9th May 2006

    Western blot - Anti-TBR2 / Eomes antibody (ab23345)

    ab23345 detects a clear band of ~ 72 kDa in lysates from EL4 cells expressing V5 tagged Eomesodermin (lane 2). Lanes 1 and 3 contain lysates from EL4 cells expressing empty vector or V5 tag alone. Lanes 4-6 show the same lysates blotted with anti-V5 tag antibody. GAPDH was used as a loading control.

    Lanes 1 - 3: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1/2000 dilution

    Lanes 4 - 6: V5 antibody

    Lanes 1 and 4: EL4 cells + empty vector

    Lanes 2 and 5: EL4 cells + vector expressing V5 tagged Eomesodermin

    Lanes 3 and 6: EL4 cells + V5 tagged vector

    Secondary

    All lanes: Goat anti Rabbit at 1/2500 dilution

    Predicted band size: 72 kDa

    Observed band size: 72 kDa

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail

    Western blot - Anti-TBR2 / Eomes antibody (ab23345)

    All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/mL

    All lanes: Human Mesendoderm (Day 2) Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 72 kDa

    Observed band size: 74 kDa, 85 kDa

  • Western blot - Anti-TBR2 / Eomes antibody (ab23345), expandable thumbnail
    This image is a courtesy of Anonymous customer review

    Western blot - Anti-TBR2 / Eomes antibody (ab23345)

    All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1/1000 dilution

    All lanes: Lysate prepared from mouse embryonic brain tissue at 20 µg

    Secondary

    All lanes: HRP-conjugated goat polyclonal to rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa

    Observed band size: 72 kDa

    Exposure time: 5min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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