Anti-TBR2 / Eomes antibody ab23345 is a rabbit polyclonal antibody that is used in TBR2 / Eomes western blotting and IHC. Suitable for human and mouse samples.
- Tried and trusted by researchers since 2006
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
WB | IHC-Fr | |
---|---|---|
Human | Tested | Expected |
Mouse | Expected | Tested |
Rat | Predicted | Predicted |
Common marmoset | Predicted | Predicted |
Cow | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.4-2.5 µg/mL | Notes Abcam recommends using milk as the blocking agent. In our hands, when tested in western blot, this product typically gives a weaker signal in mouse tissue lysates compared to human cell lines. However, this product gives clean, specific staining in IHC-Fr on mouse E14.5 cortex. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow, Common marmoset | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes Abcam recommends the following antigen retrieval method: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow, Common marmoset | Dilution info - | Notes - |
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Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
Tbr2, Eomes, Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2
Anti-TBR2 / Eomes antibody ab23345 is a rabbit polyclonal antibody that is used in TBR2 / Eomes western blotting and IHC. Suitable for human and mouse samples.
- Tried and trusted by researchers since 2006
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Tbr2 expression is observed in neuron progenitor compartments in development (the subventricular zone and ventricular zone) and expression rises and falls with cortical plate neurogenesis. Transition from radial glia to intermediate progenitor cell is associated with upregulation of Tbr2.
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This supplementary information is collated from multiple sources and compiled automatically.
TBR2 also known as Eomes or Eomesodermin is a transcription factor with a molecular mass of approximately 72 kDa. It is a member of the T-box family and is expressed predominantly in the developing central nervous system especially in the intermediate progenitor cells in the subventricular zone of the cortex. TBR2 plays a significant role in neurogenesis by regulating the proliferation and differentiation of neuronal progenitor cells. This regulation ensures proper development of specific neural circuits critical for overall brain function.
TBR2 is important in controlling the transition of neural progenitors during cortical development. It serves as an important marker for intermediate neuronal precursors highlighting its importance in the progression from radial glia to neurons. TBR2 does not function in a complex but works closely with the transcription factor Neurogenin 2 to control neuronal differentiation. Its activity determines the timing of neuronal differentiation making it indispensable for orderly cortical layer formation.
TBR2 is involved in the Wnt signaling and Notch signaling pathways both key regulators of neurodevelopment. In the Wnt pathway TBR2 influences the balance between progenitor cell proliferation and differentiation by interacting with proteins such as Beta-catenin. In the Notch signaling pathway TBR2 modulates neural precursor cell fate decisions often in relation with proteins like DLL1. These pathways ensure proper brain structure and function by coordinating neural progenitor activity.
TBR2 mutations or misregulation can lead to neurodevelopmental disorders such as microcephaly and intellectual disabilities. The protein's association with these conditions reflects its critical role in cortical development and neuronal differentiation. TBR2's dysregulation is often observed alongside the malfunction of its pathway partners like Beta-catenin in the development of such disorders illustrating the intricate interplay of genetic and molecular factors underlying these diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Dlx2 and Tbr2 identify non overlapping progenitor lineages in the adult mouse SVZ (subventricular zone).
(A–B) Adult C57/BL6 mouse forebrain coronal sections at rostro-caudal point 1.2 relative to the bregma were immunostained for Dcx (red), Dlx2 or Tbr2 (green) and Ki67 (blue). Both progenitor populations show characteristics of migrating neuroblasts, as indicated by their Dcx expression (C–D). Adult Mash1 mouse forebrain coronal sections at rostro-caudal point 1.2 relative to the bregma were immunostained for Tbr2 (red) and Dlx2 (blue). Both Tbr2 and Dlx2 exhibited EGFP expression, but showed no colocalisation. Right side captions show cropped individual channels and the merges. Full panel insets are zoomed and cropped DAB stained photomicrographs of rostral periventricular sections for Tbr2 in the dorsal SVZ (A), Dlx2 in the dorso-lateral SVZ (B) and Mash1 in the ventro-lateral SVZ (C). Yellow arrows and arrowheads show respectively positive stained cell and low level TF staining. Dotted lines mark approximate boundaries of ventricular space. Flattened confocal z-stacks are of 14–15 μm thickness, including captions. Scale bars: 15 μm in full panels, 20 μm in captions and 25 μm in insets.
Gel type: MOPS
Blocking buffer: 1% milk
All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/mL
Lane 1: Human PTA-6967 Whole Cell Lysate at 20 µg
Lane 2: E14 Mouse Embryo Brain Tissue Lysate at 40 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 75 kDa, 85 kDa
Exposure time: 12min
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab23345 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
Abcam recommends using milk as the blocking agent.
All lanes: ab23345 at 1 mg/mL
All lanes: Human ES Cells treated with Retinoic Acid (48h) at 20 µg
All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 50 kDa, 65 kDa, 75 kDa, 85 kDa
Exposure time: 20min
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab23345 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
Abcam recommends using milk as the blocking agent.
All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/mL
All lanes: Human ES Cells treated with Retinoic Acid (24h) at 20 µg
All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa, 85 kDa
Exposure time: 20min
IHC image of TRB2 staining in a mouse brain E14 frozen tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.2% (v/v) Triton X-100 for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.05% (v/v) Triton X-100 and 1% (w/v) BSA with ab23345 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used to detect the primary antibody. The section was mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/mL
Lane 1: Human Mesendoderm (Day 2) Whole Cell Lysate at 10 µg
Lane 2: E14 Mouse Embryo Brain Tissue Lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa, 85 kDa
Exposure time: 4min
ab23345 staining mouse developing cerebral cortex tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TX-100 prior to blocking with 2.5% serum for 1 hour at RT. The primary antibody was diluted 1/500 and incubated with the sample for 18 hours. A biotinylated pig anti-rabbit IgG antibody, diluted 1/500, was used as the secondary.
ab23345 staining TBR2 / Eomes in mouse embryonic brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 1% BSA and normal Goat serum for 30 minutes at RT. The sample was incubated with primary antibody (1/1000 in TBS + BSA 1%) for 10 hours at 40C. An Alexa Fluor® 555-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/800 dilution.
ab23345 detects a clear band of ~ 72 kDa in lysates from EL4 cells expressing V5 tagged Eomesodermin (lane 2). Lanes 1 and 3 contain lysates from EL4 cells expressing empty vector or V5 tag alone. Lanes 4-6 show the same lysates blotted with anti-V5 tag antibody. GAPDH was used as a loading control.
Lanes 1 - 3: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1/2000 dilution
Lanes 4 - 6: V5 antibody
Lanes 1 and 4: EL4 cells + empty vector
Lanes 2 and 5: EL4 cells + vector expressing V5 tagged Eomesodermin
Lanes 3 and 6: EL4 cells + V5 tagged vector
All lanes: Goat anti Rabbit at 1/2500 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1 µg/mL
All lanes: Human Mesendoderm (Day 2) Whole Cell Lysate at 20 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 74 kDa, 85 kDa
All lanes: Western blot - Anti-TBR2 / Eomes antibody (ab23345) at 1/1000 dilution
All lanes: Lysate prepared from mouse embryonic brain tissue at 20 µg
All lanes: HRP-conjugated goat polyclonal to rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 5min
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