AB275960

Anti-TBR2 / Eomes antibody [BLR104H] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal TBR2 / Eomes antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human EOMES aa 100-200.

View Alternative Names

TBR2, EOMES, Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

Formalin-fixed, paraffin-embedded non-Hodgkin's B-cell lymphoma tissue stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.

Supplier Data

Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunocytochemistry. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.

Supplier Data

Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NK-92 cells was
prepared using NETN lysis buffer. ab275960 used for IP at 6 μl per reaction. For blotting immunoprecipitated TBR2 / Eomes, ab275960 was used at 1/1000 dilution.

Lane 1 : ab275960 IP

Lane 2 : Control IgG IP

Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

All lanes:

Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 4:

NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 72 kDa

false

Exposure time: 75s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR104H

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB, ICC

applications

Immunogen

Synthetic Peptide within Human EOMES aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

O95936

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "6 μl/1 mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

style

left-column

Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99.91% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBR2 also known as Eomes or Eomesodermin is a transcription factor with a molecular mass of approximately 72 kDa. It is a member of the T-box family and is expressed predominantly in the developing central nervous system especially in the intermediate progenitor cells in the subventricular zone of the cortex. TBR2 plays a significant role in neurogenesis by regulating the proliferation and differentiation of neuronal progenitor cells. This regulation ensures proper development of specific neural circuits critical for overall brain function.

Biological function summary

TBR2 is important in controlling the transition of neural progenitors during cortical development. It serves as an important marker for intermediate neuronal precursors highlighting its importance in the progression from radial glia to neurons. TBR2 does not function in a complex but works closely with the transcription factor Neurogenin 2 to control neuronal differentiation. Its activity determines the timing of neuronal differentiation making it indispensable for orderly cortical layer formation.

Pathways

TBR2 is involved in the Wnt signaling and Notch signaling pathways both key regulators of neurodevelopment. In the Wnt pathway TBR2 influences the balance between progenitor cell proliferation and differentiation by interacting with proteins such as Beta-catenin. In the Notch signaling pathway TBR2 modulates neural precursor cell fate decisions often in relation with proteins like DLL1. These pathways ensure proper brain structure and function by coordinating neural progenitor activity.

TBR2 mutations or misregulation can lead to neurodevelopmental disorders such as microcephaly and intellectual disabilities. The protein's association with these conditions reflects its critical role in cortical development and neuronal differentiation. TBR2's dysregulation is often observed alongside the malfunction of its pathway partners like Beta-catenin in the development of such disorders illustrating the intricate interplay of genetic and molecular factors underlying these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.

See full target information EOMES

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2404432 PubMed39573933

2024

γδ T Cell-mediated Tumor Immunity is Tightly Regulated by STING and TGF-β Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Luo,Shengli Wang,Quanli Yang,Qianqian Fu,Chuyun Zhu,Tao Li,Shuxian Yang,Yin Zhao,Rong Guo,Xiaosong Ben,Yuzhen Zheng,Sitao Li,Guang Yang,Hongru Zhang,Hui Xiao,Zhengfan Jiang,Nan Yan,Dieter Kabelitz,Guodong Sun,Zvi Granot,Ligong Lu,Fuping You,Jianlei Hao,Zhinan Yin

Nature biotechnology 43:52-62 PubMed38418648

2024

Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Meiyan Wang,Lei Zhang,Sammy Weiser Novak,Jingting Yu,Iryna S Gallina,Lynne L Xu,Christina K Lim,Sarah Fernandes,Maxim N Shokhirev,April E Williams,Monisha D Saxena,Shashank Coorapati,Sarah L Parylak,Cristian Quintero,Elsa Molina,Leonardo R Andrade,Uri Manor,Fred H Gage

Frontiers in immunology 12:778329 PubMed34975867

2021

Colorectal Cancer-Associated Immune Exhaustion Involves T and B Lymphocytes and Conventional NK Cells and Correlates With a Shorter Overall Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Sorrentino,Luigi D'Antonio,Cristiano Fieni,Stefania Livia Ciummo,Emma Di Carlo

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TBR2 / Eomes antibody [BLR104H] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise