Rabbit Recombinant Monoclonal TBR2 / Eomes antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EOMES aa 100-200.
Images
![Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--immunoprecipitation-img129594.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)")
![Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--immunocytochemistry-img76835.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)")
![Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--western-blot-img64227.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131704.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113629.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (AB275960)")
Publications
Filter by
- Frontiers in immunology 12:7783292021Colorectal Cancer-Associated Immune Exhaustion Involves T and B Lymphocytes and Conventional NK Cells and Correlates With a Shorter Overall Survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlo Sorrentino,Luigi D'Antonio,Cristiano Fieni,Stefania Livia Ciummo,Emma Di CarloPubMed 34975867
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99.91% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human EOMES aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link O95936
Reactivity data
Select an application| IHC-P | ICC | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 6 μl/1 mg lysate |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Target data
Function
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
Alternative names
TBR2, EOMES, Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2
Recommended products
Rabbit Recombinant Monoclonal TBR2 / Eomes antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EOMES aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99.91% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human EOMES aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link O95936
- Clone number
- BLR104H
- Purity
- IgG fraction
- Concentration
- Purification notes
Recombinant antibody was purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TBR2 also known as Eomes or Eomesodermin is a transcription factor with a molecular mass of approximately 72 kDa. It is a member of the T-box family and is expressed predominantly in the developing central nervous system especially in the intermediate progenitor cells in the subventricular zone of the cortex. TBR2 plays a significant role in neurogenesis by regulating the proliferation and differentiation of neuronal progenitor cells. This regulation ensures proper development of specific neural circuits critical for overall brain function.
- Biological function summary
TBR2 is important in controlling the transition of neural progenitors during cortical development. It serves as an important marker for intermediate neuronal precursors highlighting its importance in the progression from radial glia to neurons. TBR2 does not function in a complex but works closely with the transcription factor Neurogenin 2 to control neuronal differentiation. Its activity determines the timing of neuronal differentiation making it indispensable for orderly cortical layer formation.
- Pathways
TBR2 is involved in the Wnt signaling and Notch signaling pathways both key regulators of neurodevelopment. In the Wnt pathway TBR2 influences the balance between progenitor cell proliferation and differentiation by interacting with proteins such as Beta-catenin. In the Notch signaling pathway TBR2 modulates neural precursor cell fate decisions often in relation with proteins like DLL1. These pathways ensure proper brain structure and function by coordinating neural progenitor activity.
- Associated diseases and disorders
TBR2 mutations or misregulation can lead to neurodevelopmental disorders such as microcephaly and intellectual disabilities. The protein's association with these conditions reflects its critical role in cortical development and neuronal differentiation. TBR2's dysregulation is often observed alongside the malfunction of its pathway partners like Beta-catenin in the development of such disorders illustrating the intricate interplay of genetic and molecular factors underlying these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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5 product images
![Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--immunoprecipitation-img129594.jpg "Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)")
Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)
Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NK-92 cells was
prepared using NETN lysis buffer. ab275960 used for IP at 6 μl per reaction. For blotting immunoprecipitated TBR2 / Eomes, ab275960 was used at 1/1000 dilution.Lane 1: ab275960 IP
Lane 2: Control IgG IP
Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)
Predicted band size: 72 kDa
![Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--immunocytochemistry-img76835.jpg "Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)")
Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)
NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunocytochemistry. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
![Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--western-blot-img64227.jpg "Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)")
Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)
All lanes: Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 4: NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 72 kDa
Exposure time: 75s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131704.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)
Formalin-fixed, paraffin-embedded non-Hodgkin's B-cell lymphoma tissue stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-blr104h-bsa-free-ab275960--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113629.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
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