Anti-TBR2 / Eomes antibody [EPR19012] (ab183991) is a rabbit monoclonal antibody that is used to detect TBR2 / Eomes in IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 50 publications
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img51740.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991)")
![Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunocytochemistry-immunofluorescence-img68630.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991)")
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-frozen-sections-img117305.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4337.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991)")
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-frozen-sections-img78432.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (AB183991)")
Publications
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- Science advances 9:eadf27722023Neurodevelopmental defects in human cortical organoids with -acetylneuraminic acid synthase mutation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Bu,Yanping Dai,Huaqin Zhang,Min Li,Haxiaoyu Liu,Yan Huang,Ailing Zeng,Feng Qin,Linhong Jiang,Liang Wang,Yaxing Chen,Hongchun Li,Xiaojie Wang,Yue Zhao,Meng Qin,Ying Zhao,Ni Zhang,Weihong Kuang,Yinglan Zhao,Xiaobo CenPubMed 38000033
- Nature communications 14:56652023MYC Deregulation and PTEN Loss Model Tumor and Stromal Heterogeneity of Aggressive Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZinab O Doha,Xiaoyan Wang,Nicholas L Calistri,Jennifer Eng,Colin J Daniel,Luke Ternes,Eun Na Kim,Carl Pelz,Michael Munks,Courtney Betts,Sunjong Kwon,Elmar Bucher,Xi Li,Trent Waugh,Zuzana Tatarova,Dylan Blumberg,Aaron Ko,Nell Kirchberger,Jennifer A Pietenpol,Melinda E Sanders,Ellen M Langer,Mu-Shui Dai,Gordon Mills,Koei Chin,Young Hwan Chang,Lisa M Coussens,Joe W Gray,Laura M Heiser,Rosalie C SearsPubMed 37704631
- Nature communications 14:43752023Platelet-derived exerkine CXCL4/platelet factor 4 rejuvenates hippocampal neurogenesis and restores cognitive function in aged mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOdette Leiter,David Brici,Stephen J Fletcher,Xuan Ling Hilary Yong,Jocelyn Widagdo,Nicholas Matigian,Adam B Schroer,Gregor Bieri,Daniel G Blackmore,Perry F Bartlett,Victor Anggono,Saul A Villeda,Tara L WalkerPubMed 37587147
- Nature communications 14:48932023Camrelizumab combined with apatinib in patients with first-line platinum-resistant or PD-1 inhibitor resistant recurrent/metastatic nasopharyngeal carcinoma: a single-arm, phase 2 trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Yuan,Guo-Dong Jia,Xiao-Fei Lv,Si-Yi Xie,Shan-Shan Guo,Da-Feng Lin,Li-Ting Liu,Dong-Hua Luo,Yi-Fu Li,Shen-Wen Deng,Ling Guo,Mu-Sheng Zeng,Xiu-Yu Cai,Sai-Lan Liu,Xue-Song Sun,Xiao-Yun Li,Su-Chen Li,Qiu-Yan Chen,Lin-Quan Tang,Hai-Qiang MaiPubMed 37580352
- iScience 26:1069092023Integrative systems biology characterizes immune-mediated neurodevelopmental changes in murine Zika virus microcephaly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKimino Fujimura,Amanda J Guise,Tojo Nakayama,Christoph N Schlaffner,Anais Meziani,Mukesh Kumar,Long Cheng,Dylan J Vaughan,Andrew Kodani,Simon Van Haren,Kenneth Parker,Ofer Levy,Ann F Durbin,Irene Bosch,Lee Gehrke,Hanno Steen,Ganeshwaran H Mochida,Judith A SteenPubMed 37332674
- International journal of molecular sciences 24:2023Tip60/KAT5 Histone Acetyltransferase Is Required for Maintenance and Neurogenesis of Embryonic Neural Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaoru Tominaga,Eiji Sakashita,Katsumi Kasashima,Kenji Kuroiwa,Yasumitsu Nagao,Naoki Iwamori,Hitoshi EndoPubMed 36768434
- PloS one 17:e02781472022Pax6 mutant cerebral organoids partially recapitulate phenotypes of Pax6 mutant mouse strains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNurfarhana Ferdaos,Sally Lowell,John O MasonPubMed 36441708
- iScience 25:1049082022mA regulation of cortical and retinal neurogenesis is mediated by the redundant mA readers YTHDFs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFugui Niu,Pengfei Che,Zhuoxuan Yang,Jian Zhang,Lixin Yang,Mengru Zhuang,Xijun Ou,Sheng-Jian JiPubMed 36039295
- Cell reports 39:1108692022Phosphatidylinositol transfer protein/planar cell polarity axis regulates neocortical morphogenesis by supporting interkinetic nuclear migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhigang Xie,Vytas A BankaitisPubMed 35649377
- Cancer research and treatment 55:167-1782022Molecular and Immune Profiling of Syngeneic Mouse Models Predict Response to Immune Checkpoint Inhibitors in Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDagyeong Lee,Junyong Choi,Hye Jeong Oh,In-Hye Ham,Sung Hak Lee,Sachiyo Nomura,Sang-Uk Han,Hoon HurPubMed 35609622
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IHC-P | IHC-Fr | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
Alternative names
Tbr2, Eomes, Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2
Recommended products
Anti-TBR2 / Eomes antibody [EPR19012] (ab183991) is a rabbit monoclonal antibody that is used to detect TBR2 / Eomes in IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 50 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19012
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TBR2 / Eomes antibody [EPR19012] (ab183991) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), in Mouse samples.
Recommended positive controls
IHC-P: Mouse hippocampus and E14 cortex tissues; IHC-Fr: Mouse E14.5 cortex and hippocampus tissues; ICC/IF: Mouse primary neuron cells.
Trusted by the scientific community
Anti-TBR2 / Eomes [EPR19012] (ab183991) was first used in a scientific publication in 2016 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-TBR2 / Eomes [EPR19012] (ab183991) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR19012] also available for your convenience:
ab183991, Carrier free - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free ab222226, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-TBR2 / Eomes antibody [EPR19012] ab315133, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-TBR2 / Eomes antibody [EPR19012] ab315356
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TBR2 also known as Eomes or Eomesodermin is a transcription factor with a molecular mass of approximately 72 kDa. It is a member of the T-box family and is expressed predominantly in the developing central nervous system especially in the intermediate progenitor cells in the subventricular zone of the cortex. TBR2 plays a significant role in neurogenesis by regulating the proliferation and differentiation of neuronal progenitor cells. This regulation ensures proper development of specific neural circuits critical for overall brain function.
- Biological function summary
TBR2 is important in controlling the transition of neural progenitors during cortical development. It serves as an important marker for intermediate neuronal precursors highlighting its importance in the progression from radial glia to neurons. TBR2 does not function in a complex but works closely with the transcription factor Neurogenin 2 to control neuronal differentiation. Its activity determines the timing of neuronal differentiation making it indispensable for orderly cortical layer formation.
- Pathways
TBR2 is involved in the Wnt signaling and Notch signaling pathways both key regulators of neurodevelopment. In the Wnt pathway TBR2 influences the balance between progenitor cell proliferation and differentiation by interacting with proteins such as Beta-catenin. In the Notch signaling pathway TBR2 modulates neural precursor cell fate decisions often in relation with proteins like DLL1. These pathways ensure proper brain structure and function by coordinating neural progenitor activity.
- Associated diseases and disorders
TBR2 mutations or misregulation can lead to neurodevelopmental disorders such as microcephaly and intellectual disabilities. The protein's association with these conditions reflects its critical role in cortical development and neuronal differentiation. TBR2's dysregulation is often observed alongside the malfunction of its pathway partners like Beta-catenin in the development of such disorders illustrating the intricate interplay of genetic and molecular factors underlying these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img51740.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on intermediate neuronal progenitors of mouse hippocampus is observed [PMID: 22553033].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunocytochemistry-immunofluorescence-img68630.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TBR2 / Eomes with ab183991 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing nuclear staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/mL) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-frozen-sections-img117305.jpg "Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Specific staining on E14.5 mouse cortex is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4337.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Immunohistochemical analysis of paraffin-embedded mouse E14 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on the cortex of mouse E14 is observed [PMID: 22553033].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-frozen-sections-img78432.jpg "Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Specific staining on adult mouse hippocampus is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-frozen-sections-img56899.jpg "Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen adult mouse kidney tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Negative control tissue; very weak staining is due to autoflorescence of the kidney section.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991), expandable thumbnail](https://content.abcam.com/products/images/tbr2-eomes-antibody-epr19012-ab183991--immunohistochemistry-frozen-sections-img56899.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
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