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Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870) is a rabbit monoclonal antibody that is used to detect TBR2 / Eomes in IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 10 publications


Images

Immunoprecipitation - Anti-TBR2 / Eomes antibody [EPR21950-241] (AB216870), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR21950-241] (AB216870), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (AB216870), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (AB216870), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (AB216870), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-FrICC/IFIPFlow CytWB
Human
Tested
Expected
Expected
Expected
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Expected
Not recommended
Not recommended
Not recommended
Cat
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Common marmoset
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Dog
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Hamster
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Pig
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Primates
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Hamster, Cat, Dog, Pig, Primates, Common marmoset
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Hamster, Cat, Dog, Pig, Primates, Common marmoset
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Hamster, Cat, Dog, Pig, Primates, Common marmoset
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Hamster, Cat, Dog, Pig, Primates, Common marmoset
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Hamster, Cat, Dog, Pig, Primates, Common marmoset
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Hamster, Cat, Dog, Pig, Primates, Common marmoset
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.

Alternative names

TBR2, EOMES, Eomesodermin homolog, T-box brain protein 2, T-brain-2, TBR-2

Recommended products

Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870) is a rabbit monoclonal antibody that is used to detect TBR2 / Eomes in IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 10 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21950-241
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870) is a rabbit recombinant monoclonal antibody and is validated for use in Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

Trusted by the scientific community


Anti-TBR2 / Eomes [EPR21950-241] (ab216870) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists


Anti-TBR2 / Eomes [EPR21950-241] (ab216870) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR21950-241] also available for your convenience:
ab216870, Carrier free - Anti-TBR2 / Eomes antibody [EPR21950-241] - BSA and Azide free ab261913, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-TBR2 / Eomes antibody [EPR21950-241] ab310988, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-TBR2 / Eomes antibody [EPR21950-241] ab311114, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-TBR2 / Eomes antibody [EPR21950-241] ab311735, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-TBR2 / Eomes antibody [EPR21950-241] ab313013, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-TBR2 / Eomes antibody [EPR21950-241] ab313217, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-TBR2 / Eomes antibody [EPR21950-241] ab321390



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TBR2 also known as Eomes or Eomesodermin is a transcription factor with a molecular mass of approximately 72 kDa. It is a member of the T-box family and is expressed predominantly in the developing central nervous system especially in the intermediate progenitor cells in the subventricular zone of the cortex. TBR2 plays a significant role in neurogenesis by regulating the proliferation and differentiation of neuronal progenitor cells. This regulation ensures proper development of specific neural circuits critical for overall brain function.

Biological function summary

TBR2 is important in controlling the transition of neural progenitors during cortical development. It serves as an important marker for intermediate neuronal precursors highlighting its importance in the progression from radial glia to neurons. TBR2 does not function in a complex but works closely with the transcription factor Neurogenin 2 to control neuronal differentiation. Its activity determines the timing of neuronal differentiation making it indispensable for orderly cortical layer formation.

Pathways

TBR2 is involved in the Wnt signaling and Notch signaling pathways both key regulators of neurodevelopment. In the Wnt pathway TBR2 influences the balance between progenitor cell proliferation and differentiation by interacting with proteins such as Beta-catenin. In the Notch signaling pathway TBR2 modulates neural precursor cell fate decisions often in relation with proteins like DLL1. These pathways ensure proper brain structure and function by coordinating neural progenitor activity.

Associated diseases and disorders

TBR2 mutations or misregulation can lead to neurodevelopmental disorders such as microcephaly and intellectual disabilities. The protein's association with these conditions reflects its critical role in cortical development and neuronal differentiation. TBR2's dysregulation is often observed alongside the malfunction of its pathway partners like Beta-catenin in the development of such disorders illustrating the intricate interplay of genetic and molecular factors underlying these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870), expandable thumbnail

    Immunoprecipitation - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

    TBR2 / Eomes was immunoprecipitated from 0.35mg Mouse E14 brain tissue lysate 10μg with ab216870 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab216870 1/1000 dilution (0.67 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Mouse E14 brain tissue lysate 10 μg

    Lane 2: ab216870 IP in Mouse E14 brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab216870 in Mouse E14 brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

    The band is consistent with what has been described in the literature (PMID: 26749212).

    All lanes: Immunoprecipitation - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

    Predicted band size: 72 kDa

    Observed band size: 85 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

    Immunocytochemistry confocal image showing nuclear staining in mouse primary neuron cells. Anti-TBR2 is stained with ab216870 in a 1/100 dilution, and 2μg/ml AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclear counterstaining is DAPI (blue).

    The negative control is Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary.

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat E14.5 cerebrum tissue labeling EOMES with ab216870 at 1/100 (5.75 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining in rat embryonic cerebrum (PMID: 24223221) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

    Immunohistochemical analysis of paraffin-embedded E14.5 rat cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on E14.5 rat cerebral cortex (PMID: 18725516) is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse E14.5 cerebrum tissue stained for EOMES using ab216870 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. The nuclear counterstain was DAPI (Blue). Positive staining in mouse embryonic cerebrum (PMID: 24223221) is observed. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

    Immunohistochemical analysis of paraffin-embedded E14.5 mouse cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on E14.5 mouse cerebral cortex (PMID: 18725516) The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR21950-241] (ab216870)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human tonsil is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

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Product protocols

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