Rabbit Polyclonal TBRG1 antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human TBRG1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TBRG1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q3YBR2
Reactivity data
Select an application| IHC-P | |
|---|---|
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes Use at 1/50 - 1/200 with fluorescent detection methods. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Acts as a growth inhibitor. Can activate p53/TP53, causes G1 arrest and collaborates with CDKN2A to restrict proliferation, but does not require either protein to inhibit DNA synthesis. Redistributes CDKN2A into the nucleoplasm. Involved in maintaining chromosomal stability.
Alternative names
NIAM, TBRG1, Transforming growth factor beta regulator 1, Nuclear interactor of ARF and Mdm2
Recommended products
Rabbit Polyclonal TBRG1 antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human TBRG1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TBRG1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q3YBR2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TBRG1 also known as Transforming Growth Factor Beta Regulator 1 or TBRG4 is a protein with a molecular mass of approximately 94 kDa. The gene encoding TBRG1 is located on chromosome 11 and the protein is expressed in various tissues with noticeable expression in the liver lungs and kidneys. Mechanically TBRG1 functions as a nucleolar protein. It interacts with other proteins within the nucleolus where it regulates cellular growth and proliferation by controlling ribosomal RNA biogenesis and ribosome assembly.
- Biological function summary
TBRG1 has significant roles in regulating cell cycle progression and apoptosis. It acts as a checkpoint mediator ensuring that cells do not proceed through the cell cycle with damaged DNA. TBRG1 may also be part of larger protein complexes which help maintain cellular homeostasis and prevent uncontrolled cell division. By mediating these important processes TBRG1 ensures proper cellular turnover and prevents aberrant cell growth contributing to tissue integrity.
- Pathways
TBRG1 prominently participates in the p53 signaling pathway and the TGF-beta signaling pathway. In the p53 pathway TBRG1 functions alongside the tumor suppressor protein p53 to induce cell cycle arrest or apoptosis in response to DNA damage. In the TGF-beta signaling pathway TBRG1 modulates the activity of SMAD proteins which are important for transmitting signals from the cell surface to the nucleus. These interactions highlight the coordination between TBRG1 and other key proteins maintaining the balance between cell survival and death.
- Associated diseases and disorders
TBRG1's involvement in the p53 and TGF-beta pathways links it to cancer and fibrosis. Aberrant expression or mutation of TBRG1 can disrupt normal cell cycle control leading to tumorigenesis notably in liver and lung cancers. Moreover dysregulation of TBRG1 in the TGF-beta pathway can promote excess collagen deposition contributing to fibrosis. The relationship between TBRG1 p53 and SMAD proteins illustrates the protein's role in these complex diseases offering potential avenues for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBRG1 antibody (ab203276)
Immunohistochemical analysis of formalin-fixed and paraffin embedded rat pancreas tissue labeling TBRG1 using ab203276 at 1/200, followed by secondary detection and DAB staining.
Downloads
Product protocols
- Visit the general protocols
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