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AB99317

Anti-TBRG4 antibody

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(1 Publication)

Rabbit Polyclonal TBRG4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TBRG4 aa 1-100.

View Alternative Names

CPR2, FASTKD4, KIAA0948, TBRG4, FAST kinase domain-containing protein 4, Cell cycle progression restoration protein 2, Protein TBRG4, Transforming growth factor beta regulator 4, Cell cycle progression protein 2

2 Images
Immunoprecipitation - Anti-TBRG4 antibody (AB99317)
  • IP

Unknown

Immunoprecipitation - Anti-TBRG4 antibody (AB99317)

Detection of TBRG4 by Western Blot of Immunprecipitate.
ab99317 at 0.1 µg/ml staining TBRG4 in HeLa whole cell lysate immunoprecipitated using ab99317 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TBRG4 antibody (ab99317)

Predicted band size: 71 kDa

false

Western blot - Anti-TBRG4 antibody (AB99317)
  • WB

Unknown

Western blot - Anti-TBRG4 antibody (AB99317)

All lanes:

Western blot - Anti-TBRG4 antibody (ab99317) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 71 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human TBRG4 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q969Z0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab99317 was affinity purified using an epitope specific to TBRG4 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBRG4 also known as FAM195A or BRG1-Associated Factor-53A is a protein with a molecular mass of approximately 144 kDa. It participates in the regulation of cellular homeostasis and function by interacting with other proteins in the cell. Expression of TBRG4 is not limited to a specific tissue as it can be detected in multiple tissues across the body including brain and immune cells. Due to its versatile expression TBRG4 likely takes part in general cellular mechanisms across different cell types.
Biological function summary

TBRG4 influences several cellular processes through its involvement in protein interactions. It is an integral part of large molecular complexes that regulate various gene expression and remodeling of chromatin. TBRG4 often functions in concert with other components to ensure accurate modulation of transcriptional activity. Complex formation affects both direct regulation of target genes and the structural rearrangements necessary for cellular homeostasis.

Pathways

The protein plays a role in the cellular response to stress and metabolic signaling. It acts within the mTOR signaling pathway which is central to the regulation of growth and metabolism. TBRG4 interacts with other proteins like Raptor and mLST8 to modulate mTOR complex functions. Additionally it is related to the PKC signaling pathway where it may indirectly influence cellular responses to external stimuli through modulation of other participating proteins.

TBRG4 connects to both cancer and neurodegenerative diseases due to its role in cell growth and stress response. Abnormal expression or malfunction of TBRG4 may contribute to the progression of certain cancers reflecting its interaction with proteins like p53 in tumor suppression pathways. In neurodegenerative conditions alterations in TBRG4-related pathways might exacerbate pathological features particularly through dysregulated signaling mechanisms involving proteins such as Tau.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in processing of mitochondrial RNA precursors and in stabilization of a subset of mature mitochondrial RNA species, such as MT-CO1, MT-CO2, MT-CYB, MT-CO3, MT-ND3, MT-ND5 and MT-ATP8/6. May play a role in cell cycle progression (PubMed : 9383053).
See full target information TBRG4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3497 PubMed34108453

2021

Site-specific ubiquitylation acts as a regulator of linker histone H1.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Höllmüller,Simon Geigges,Marie L Niedermeier,Kai-Michael Kammer,Simon M Kienle,Daniel Rösner,Martin Scheffner,Andreas Marx,Florian Stengel
View all publications
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Product promise

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