AB109313

Anti-TBX1 antibody [EPR3289(2)]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal TBX1 antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 10 publications.

View Alternative Names

T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein, TBX1

3 Images

Unknown

Western blot - Anti-TBX1 antibody [EPR3289(2)] (AB109313)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/2000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-TBX1 antibody [EPR3289(2)] (AB109313)

Blocking buffer : 5% NFDM/TBST.

There is a strong non-specific band in human liver. Human liver is negative control for TBX1 which is consistent with PMID : 28920943.

All lanes:

Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) control cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) nuclear cell lysate at 20 µg

Lane 3:

Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Exposure time: 10s

Unknown

Western blot - Anti-TBX1 antibody [EPR3289(2)] (AB109313)

Blocking buffer : 5% NFDM/TBST.

Exposure time : 30 seconds (Lane 1) and 180 seconds (Lane 2).

Lane 1:

Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/2000 dilution

Lane 2:

Western blot - Anti-TBX1 antibody [EPR3289(2)] (ab109313) at 1/5000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Carrier free

Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3289(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBX1 also known as T-box protein 1 is a transcription factor with a molecular weight of around 58 kDa. It belongs to the T-box gene family and plays a role in regulating transcriptional activity of specific genes. TBX1 expresses mostly in mesodermal and neural crest cell derivatives during embryonic development. High expression levels occur in the pharyngeal arches which are structures important for the development of facial and neck regions.

Biological function summary

This protein influences developmental processes involving tissues derived from the pharyngeal arches. TBX1 functions as a part of a regulatory network important for proper embryogenesis. It does not form part of a stable complex but interacts with many cofactors to direct the transcription of genes responsible for cell proliferation differentiation and migration. The correct functioning of TBX1 ensures normal formation and patterning of the craniofacial structures cardiac outflow tract and great vessels.

Pathways

TBX1 engages in the retinoic acid signaling pathway which is essential for the regulation of gene expression during development. It also plays an important role in the fibroblast growth factor (FGF) signaling pathway. These pathways involve related proteins like FGF8 and FGF10 which are important for coordinated signaling during morphogenesis. TBX1's regulatory influence on these pathways highlights its importance in developmental signaling networks.

TBX1 is most notably associated with DiGeorge syndrome a condition resulting from deletions on chromosome 22q11.2 which impacts multiple systems including the heart and immune system. Mutations or deletions affecting TBX1 are linked with the syndrome's characteristic features such as heart defects and craniofacial abnormalities. Additionally disruptions in TBX1 may connect to congenital heart disease interacting with other proteins similarly implicated in structural heart defects during embryonic development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays a key role in cardiovascular development by promoting pharyngeal arch segmentation during embryonic development (By similarity). Also involved in craniofacial muscle development (By similarity). Together with NKX2-5, acts as a regulator of asymmetric cardiac morphogenesis by promoting expression of PITX2 (By similarity). Acts upstream of TBX1 for the formation of the thymus and parathyroid glands from the third pharyngeal pouch (By similarity). Required for hair follicle stem cell self-renewal (By similarity). Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence (PubMed : 11111039, PubMed : 22095455).

See full target information TBX1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23079 PubMed37410022

2023

Genistein-mediated thermogenesis and white-to-beige adipocyte differentiation involve transcriptional activation of cAMP response elements in the Ucp1 promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeca Fuentes-Romero,Laura A Velázquez-Villegas,Sarai Vasquez-Reyes,Berenice Pérez-Jiménez,Zuleima N Domínguez Velázquez,Mónica Sánchez-Tapia,Ariana Vargas-Castillo,Sandra Tobón-Cornejo,Adriana M López-Barradas,Valentín Mendoza,Nimbe Torres,Fernando López-Casillas,Armando R Tovar

Journal of cellular and molecular medicine 25:9953-9971 PubMed34676967

2021

Dihydromyricetin resists inflammation-induced muscle atrophy via ryanodine receptor-CaMKK-AMPK signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lianjie Hou,Fangyi Jiang,Bo Huang,Weijie Zheng,Yufei Jiang,Gengyuan Cai,Dewu Liu,Ching Yuan Hu,Chong Wang

Oxidative medicine and cellular longevity 2021:8542809 PubMed34650665

2021

Dihydromyricetin Ameliorates Inflammation-Induced Insulin Resistance via Phospholipase C-CaMKK-AMPK Signal Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lianjie Hou,Fangyi Jiang,Bo Huang,Weijie Zheng,Yufei Jiang,Gengyuan Cai,Dewu Liu,Ching Yuan Hu,Chong Wang

Clinical and translational medicine 11:e374 PubMed33783987

2021

VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu-Yue Lin,Yun-Long Zhang,Jie Bai,Jin-Qiu Liu,Hui-Hua Li

Frontiers in oncology 10:616173 PubMed33575219

2021

TBX1 Functions as a Tumor Activator in Prostate Cancer by Promoting Ribosome RNA Gene Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Cui,Yamin Zhang,Xiaoyue Ren,Lei Jin,Hongyi Zhang

Scientific reports 10:14589 PubMed32883997

2020

Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Keya Meyers,María López,Joanna Ho,Savannah Wills,Srujana Rayalam,Shashidharamurthy Taval

International journal of molecular medicine 45:956-966 PubMed31922246

2020

MicroRNA‑3651 promotes colorectal cancer cell proliferation through directly repressing T‑box transcription factor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Changfeng Li,Dayong Ding,Yongjian Gao,Yongchao Li

Cellular physiology and biochemistry : internation 48:556-568 PubMed30021212

2018

Browning of Pig White Preadipocytes by Co-Overexpressing Pig PGC-1α and Mice UCP1.

Applications

Unspecified application

Species

Unspecified reactive species

Lianjie Hou,Meiying Xie,Lingbo Cao,Jia Shi,Guli Xu,Chingyuan Hu,Chong Wang

Nature communications 9:245 PubMed29339725

2018

TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A Velazquez-Villegas,Alessia Perino,Vera Lemos,Marika Zietak,Mitsunori Nomura,Thijs Willem Hendrik Pols,Kristina Schoonjans

Proceedings of the National Academy of Sciences of 114:10113-10118 PubMed28874583

2017

Smad7 enables STAT3 activation and promotes pluripotency independent of TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Yu,Shuchen Gu,Wenjian Li,Chuang Sun,Fenfang Chen,Mu Xiao,Lei Wang,Dewei Xu,Ye Li,Chen Ding,Zongping Xia,Yi Li,Sheng Ye,Pinglong Xu,Bin Zhao,Jun Qin,Ye-Guang Chen,Xia Lin,Xin-Hua Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TBX1 antibody [EPR3289(2)]

Western blot - Anti-TBX1 antibody [EPR3289(2)] (AB109313)

Western blot - Anti-TBX1 antibody [EPR3289(2)] (AB109313)

Western blot - Anti-TBX1 antibody [EPR3289(2)] (AB109313)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise