AB239967

Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TBX1 antibody. Carrier free. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

View Alternative Names

T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein, TBX1

3 Images

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Western blot - Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free (AB239967)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TBX1 antibody [EPR3289(2)] (<a href='/en-us/products/primary-antibodies/tbx1-antibody-epr32892-ab109313'>ab109313</a>) at 1/2000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free (AB239967)

Blocking buffer : 5% NFDM/TBST.

There is a strong non-specific band in human liver. Human liver is negative control for TBX1 which is consistent with PMID : 28920943.

All lanes:

Western blot - Anti-TBX1 antibody [EPR3289(2)] (<a href='/en-us/products/primary-antibodies/tbx1-antibody-epr32892-ab109313'>ab109313</a>) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) control cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) nuclear cell lysate at 20 µg

Lane 3:

Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Exposure time: 10s

Unknown

Western blot - Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free (AB239967)

Blocking buffer : 5% NFDM/TBST.

Exposure time : 30 seconds (Lane 1) and 180 seconds (Lane 2).

Lane 1:

Western blot - Anti-TBX1 antibody [EPR3289(2)] (<a href='/en-us/products/primary-antibodies/tbx1-antibody-epr32892-ab109313'>ab109313</a>) at 1/2000 dilution

Lane 2:

Western blot - Anti-TBX1 antibody [EPR3289(2)] (<a href='/en-us/products/primary-antibodies/tbx1-antibody-epr32892-ab109313'>ab109313</a>) at 1/5000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 53 kDa

false

Unconjugated

Anti-TBX1 antibody [EPR3289(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3289(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab239967 is the carrier-free version of ab109313.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBX1 also known as T-box protein 1 is a transcription factor with a molecular weight of around 58 kDa. It belongs to the T-box gene family and plays a role in regulating transcriptional activity of specific genes. TBX1 expresses mostly in mesodermal and neural crest cell derivatives during embryonic development. High expression levels occur in the pharyngeal arches which are structures important for the development of facial and neck regions.

Biological function summary

This protein influences developmental processes involving tissues derived from the pharyngeal arches. TBX1 functions as a part of a regulatory network important for proper embryogenesis. It does not form part of a stable complex but interacts with many cofactors to direct the transcription of genes responsible for cell proliferation differentiation and migration. The correct functioning of TBX1 ensures normal formation and patterning of the craniofacial structures cardiac outflow tract and great vessels.

Pathways

TBX1 engages in the retinoic acid signaling pathway which is essential for the regulation of gene expression during development. It also plays an important role in the fibroblast growth factor (FGF) signaling pathway. These pathways involve related proteins like FGF8 and FGF10 which are important for coordinated signaling during morphogenesis. TBX1's regulatory influence on these pathways highlights its importance in developmental signaling networks.

TBX1 is most notably associated with DiGeorge syndrome a condition resulting from deletions on chromosome 22q11.2 which impacts multiple systems including the heart and immune system. Mutations or deletions affecting TBX1 are linked with the syndrome's characteristic features such as heart defects and craniofacial abnormalities. Additionally disruptions in TBX1 may connect to congenital heart disease interacting with other proteins similarly implicated in structural heart defects during embryonic development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays a key role in cardiovascular development by promoting pharyngeal arch segmentation during embryonic development (By similarity). Also involved in craniofacial muscle development (By similarity). Together with NKX2-5, acts as a regulator of asymmetric cardiac morphogenesis by promoting expression of PITX2 (By similarity). Acts upstream of TBX1 for the formation of the thymus and parathyroid glands from the third pharyngeal pouch (By similarity). Required for hair follicle stem cell self-renewal (By similarity). Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence (PubMed : 11111039, PubMed : 22095455).

See full target information TBX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 30:666-679 PubMed35170865

2022

Exosomal miR-122 promotes adipogenesis and aggravates obesity through the VDR/SREBF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Huang,Ji-Xiong Chen,Yi Ren,Li-Chun Fan,Wei Xiang,Xiao-Jie He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free

Western blot - Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free (AB239967)

Western blot - Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free (AB239967)

Western blot - Anti-TBX1 antibody [EPR3289(2)] - BSA and Azide free (AB239967)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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