Rabbit Polyclonal TBX18 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 12 publications.
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- STAR protocols 4:1022562023Protocol to generate cardiac pericytes from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengcheng Shen,Shane Rui Zhao,Yaser Khokhar,Li Li,Yang Zhou,Chun Liu,Joseph C WuPubMed 37119139
- Stem cell research & therapy 13:3242022RA signaling pathway combined with Wnt signaling pathway regulates human-induced pluripotent stem cells (hiPSCs) differentiation to sinus node-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Yin,Feng-Yuan Wang,Wei Zhang,Xi Wang,Yan-Hong Tang,Teng Wang,Yu-Ting Chen,Cong-Xin HuangPubMed 35851424
- International journal of stem cells 15:247-2572022The Biphasic Effect of Retinoic Acid Signaling Pathway on the Biased Differentiation of Atrial-like and Sinoatrial Node-like Cells from hiPSC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Liu,Dandan Long,Wenjun Huang,Wanling Peng,Huan Lan,Yafei Zhou,Xitong Dang,Rui ZhouPubMed 35220280
- Cell biology international 46:403-4142021Tbx18 promoted the conversion of human-induced pluripotent stem cell-derived cardiomyocytes into sinoatrial node-like pacemaker cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Hongyi Zhao,Dajun Quan,Yanhong Tang,Xi Wang,Congxin HuangPubMed 34882885
- Advanced healthcare materials 10:e21009952021Spatial Stem Cell Fate Engineering via Facile Morphogen Localization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGyuhyung Jin,Martha E Floy,Aaron D Simmons,Madeline M Arthur,Sean P PalecekPubMed 34459150
- Stem cells international 2021:88590712021Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Liu,Dinghui Wang,Tianhua Xiong,Yajie Liu,Xiaodong Jing,Jianlin Du,Qiang ShePubMed 33897781
- Stem cell research & therapy 11:2842020Enrichment differentiation of human induced pluripotent stem cells into sinoatrial node-like cells by combined modulation of BMP, FGF, and RA signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Liu et. al.PubMed 32678003
- DNA and cell biology 38:1313-13222019Transcription Factor prrx1 Promotes Brown Adipose-Derived Stem Cells Differentiation to Sinus Node-Like Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Yin et. al.PubMed 31545082
- Cell reports 28:190-201.e32019Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeibin Cai,Jing Tan,Jianyun Yan,Lu Zhang,Xiaoqiang Cai,Haiping Wang,Fang Liu,Maoqing Ye,Chen-Leng CaiPubMed 31269439
- Pharmacological research 141:443-4502019Functional biological pacemaker generation by T-Box18 protein expression via stem cell and viral delivery approaches in a murine model of complete heart block.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArmita Mahdavi Gorabi et. al.PubMed 30677516
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Expected | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Target data
Function
Acts as a transcriptional repressor involved in developmental processes of a variety of tissues and organs, including the heart and coronary vessels, the ureter and the vertebral column. Required for embryonic development of the sino atrial node (SAN) head area.
Alternative names
T-box transcription factor TBX18, T-box protein 18, TBX18
Recommended products
Rabbit Polyclonal TBX18 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of ab115262 are tested in WB. Previous batches were additionally validated in ICC. This application is still expected to work and is covered by our Abpromise guarantee.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TBX18 also called T-box transcription factor Tbx18 is a transcription factor involved in DNA binding and gene regulation. It has a molecular mass of approximately 60 kDa. TBX18 expresses predominantly in developing tissues including the heart kidneys and limbs. It plays a role during embryogenesis and contributes to the regulation of developmental processes by modulating the expression of other genes important for organ formation.
- Biological function summary
TBX18 controls the regulation of cell differentiation and development especially in the heart and skeletal system. As a member of the T-box family of genes TBX18 participates in forming transcriptional regulation complexes. Its regulatory activities ensure that specific genes are expressed at the right time and location during developmental processes. This precise control is essential for normal embryonic development.
- Pathways
TBX18 integrates into the Wnt signaling and BMP pathways which are critical for cell fate determination and organogenesis. Within these pathways TBX18 interacts with other transcription factors like Tbx5 and Pitx2 to influence heart development. Its involvement in the pathways allows it to help steer mesodermal cells towards forming specific tissues showcasing its role in guiding developmental decisions.
- Associated diseases and disorders
TBX18 has associations with congenital heart defects and limb malformations. Abnormal TBX18 expression or function can lead to structural heart anomalies with connections to other proteins like NKX2-5 and GATA4 which are also important in cardiac development. Research focuses on understanding how TBX18 mutations or dysregulation contribute to these conditions to find potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry - Anti-TBX18 antibody (ab115262)
ab115262 staining T-box transcription factor TBX18 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab115262 at 5 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-TBX18 antibody (ab115262)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab115262 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-TBX18 antibody (ab115262) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg
Lane 4: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 68 kDa, 70 kDa
Exposure time: 2min
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