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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal TBX18 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 12 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
ICC | WB | |
---|---|---|
Human | Tested | Tested |
Rat | Predicted | Predicted |
Chimpanzee | Predicted | Predicted |
Chinese hamster | Predicted | Predicted |
Cow | Predicted | Predicted |
Gorilla | Predicted | Predicted |
Macaque monkey | Predicted | Predicted |
Orangutan | Predicted | Predicted |
Pig | Predicted | Predicted |
Rabbit | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Acts as transcriptional repressor involved in developmental processes of a variety of tissues and organs, including the heart and coronary vessels, the ureter and the vertebral column. Required for embryonic development of the sino atrial node (SAN) head area.
T-box transcription factor TBX18, T-box protein 18, TBX18
Rabbit Polyclonal TBX18 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 12 publications.
T-box transcription factor TBX18, T-box protein 18, TBX18
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Replenishment batches of ab115262 are tested in WB. Previous batches were additionally validated in ICC. This application is still expected to work and is covered by our Abpromise guarantee.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
TBX18 controls the regulation of cell differentiation and development especially in the heart and skeletal system. As a member of the T-box family of genes TBX18 participates in forming transcriptional regulation complexes. Its regulatory activities ensure that specific genes are expressed at the right time and location during developmental processes. This precise control is essential for normal embryonic development.
TBX18 also called T-box transcription factor Tbx18 is a transcription factor involved in DNA binding and gene regulation. It has a molecular mass of approximately 60 kDa. TBX18 expresses predominantly in developing tissues including the heart kidneys and limbs. It plays a role during embryogenesis and contributes to the regulation of developmental processes by modulating the expression of other genes important for organ formation.
TBX18 integrates into the Wnt signaling and BMP pathways which are critical for cell fate determination and organogenesis. Within these pathways TBX18 interacts with other transcription factors like Tbx5 and Pitx2 to influence heart development. Its involvement in the pathways allows it to help steer mesodermal cells towards forming specific tissues showcasing its role in guiding developmental decisions.
TBX18 has associations with congenital heart defects and limb malformations. Abnormal TBX18 expression or function can lead to structural heart anomalies with connections to other proteins like NKX2-5 and GATA4 which are also important in cardiac development. Research focuses on understanding how TBX18 mutations or dysregulation contribute to these conditions to find potential therapeutic interventions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab115262 staining T-box transcription factor TBX18 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab115262 at 5 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab115262 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-TBX18 antibody (AB115262) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg
Lane 4: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 68 kDa, 70 kDa
Exposure time: 2min
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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