AB99302

Anti-Tbx3 antibody

Name: Anti-Tbx3 antibody. Rabbit polyclonal (ab99302) | Abcam
Brand: Abcam Limited
SKU: AB99302
Price: 450 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(40 Publications)

Rabbit Polyclonal TBX3 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 40 publications.

View Alternative Names

T-box transcription factor TBX3, T-box protein 3, TBX3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (AB99302)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (AB99302)

ab99302 staining Tbx3 in HepG2 cells. The cells were fixed with 4% Paraformaldehyde (10min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab99302 at 5μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed, at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) pre-adsorbed, at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Perkin Elmer, Operetta CLS).

ICC/IF

AbReview54433****

Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (AB99302)

ab99302 staining Tbx3 in the AR42J-B13 rat pancreatic tumor cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton/PBS and blocked with blocking buffer for 60 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/5000.

This image is courtesy of an Abreview by Zoe Burke.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tbx3 also known as T-box transcription factor 3 is a transcription factor belonging to the T-box gene family. It has a molecular weight of approximately 74 kDa. The protein functions by binding to specific DNA sequences and regulating gene expression. It is involved in the repression or activation of target genes. Tbx3 is expressed in many tissues including the heart developing limbs and mammary glands.

Biological function summary

T-box transcription factor 3 is significant in embryonic development and cellular differentiation. It plays an important role in the development of the heart and limbs impacting cell fate decisions. Tbx3 interacts with other T-box family members and it is part of a regulatory network controlling pluripotency and differentiation in stem cells. Its expression patterns suggest important functions in organogenesis and tissue specification.

Pathways

Tbx3 is a modulatory component in pathways controlling development and cell cycle progression. It is closely involved in the Wnt signaling and cell differentiation pathways. In the Wnt pathway Tbx3 regulates genes essential for cell proliferation and development. It interacts with proteins such as β-catenin linking it to other pathway members like LEF/TCF transcription factors further influencing cellular responses and development processes.

T-box transcription factor 3 has associations with certain cancers and developmental disorders. Mutations in Tbx3 can lead to ulnar-mammary syndrome a disorder that affects limb and mammary gland development. In cancer Tbx3 overexpression is linked to melanoma progression interacting with proteins like p53 to affect cell cycle regulation and apoptotic pathways. Understanding Tbx3's role may provide insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor involved in developmental processes (PubMed : 10468588). Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present in the regulatory region of several genes (PubMed : 12000749). Probably plays a role in limb pattern formation (PubMed : 10468588). Required for mammary placode induction, and maintenance of the mammary buds during development (By similarity). Involved in branching morphogenesis in both developing lungs and adult mammary glands, via negative modulation of target genes; acting redundantly with TBX2 (By similarity). Required, together with TBX2, to maintain cell proliferation in the embryonic lung mesenchyme; perhaps acting downstream of SHH, BMP and TGFbeta signaling (By similarity). Involved in modulating early inner ear development, acting independently of, and also redundantly with, TBX2 in different subregions of the developing ear (By similarity). Acts as a negative regulator of PML function in cellular senescence (PubMed : 22002537).

See full target information TBX3

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:1966-1986 PubMed39897553

2025

TBX3 shapes an immunosuppressive microenvironment and induces immunotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Liu,Chunyu Zhang,Jiatong Xiao,Yunbo He,Haisu Liang,Jinliang Huang,Zhiyong Cai,Zhenglin Yi,Mingfeng Chen,Yixiao Li,Jun Zhang,Fenglian Liu,Peng Ren,Huihuang Li,Jinbo Chen,Benyi Fan,Jiao Hu,Xiongbing Zu,Dingshan Deng

Cancer medicine 13:e70303 PubMed39403898

2024

A High-Throughput Drug Repurposing Strategy to Treat TBX2 and/or TBX3 Dependent Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna S Bleloch,Sizhu Lu,Saif Feroz Khan,Karabo Serala,Elena Seraia,Val Millar,Daniel Ebner,Colin Goding,Sharon Prince

iScience 27:109959 PubMed38832019

2024

3D model of mouse embryonic pancreas and endocrine compartment using stem cell-derived mesoderm and pancreatic progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Shlomit Edri,Vardit Rosenthal,Or Ginsburg,Abigail Newman Frisch,Christophe E Pierreux,Nadav Sharon,Shulamit Levenberg

Development (Cambridge, England) 151: PubMed38828908

2024

TBX3 is essential for establishment of the posterior boundary of anterior genes and upregulation of posterior genes together with HAND2 during the onset of limb bud development.

Applications

Unspecified application

Species

Unspecified reactive species

Geoffrey Soussi,Ausra Girdziusaite,Shalu Jhanwar,Victorio Palacio,Marco Notaro,Rushikesh Sheth,Rolf Zeller,Aimée Zuniga

Stem cell research & therapy 15:148 PubMed38778426

2024

TBX3 transfection and nodal signal pathway inhibition promote differentiation of adipose mesenchymal stem cell to cardiac pacemaker-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Faris Basalamah,Ismail Hadisoebroto Dilogo,Sunu Budhi Raharjo,Muchtaruddin Mansyur,Nuryati Chairani Siregar,Nurhadi Ibrahim,Budi Yuli Setianto,Yoga Yuniadi

Discover oncology 15:51 PubMed38413457

2024

Expression and prognosis analysis of TBX2 subfamily in human lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Mi,Qiubo Wang,Qingyang Liu,Fengying Jiang,Yuan Ji

Journal of cellular physiology 239:212-226 PubMed38149479

2023

Cadherin-5 facilitated the differentiation of human induced pluripotent stem cells into sinoatrial node-like pacemaker cells by regulating β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Fengyuan Wang,Lin Yin,Yanhong Tang,Xi Wang,Congxin Huang

Molecular biology reports 50:9935-9950 PubMed37878207

2023

Abnormal activation of the Wnt3a/β-catenin signaling pathway promotes the expression of T-box transcription factor 3(TBX3) and the epithelial-mesenchymal transition pathway to mediate the occurrence of adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Li,Ting Li,Tingting Jin,Yi Chen,Lan Cheng,Qiheng Liang,Simiao Yan,Tingting Li,Qingzhen Ran,Wanqun Chen

Nature cell biology 25:481-492 PubMed36690849

2023

Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Fung Wong,Yatendra Kumar,Martin Proks,Jose Alejandro Romero Herrera,Michaela Mrugala Rothová,Rita S Monteiro,Sara Pozzi,Rachel E Jennings,Neil A Hanley,Wendy A Bickmore,Joshua M Brickman

Cells 12: PubMed36611882

2022

Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Han Li,Hao Peng,Wei Hong,Yingying Wei,Haojun Tian,Xiaojie Huang,Linyan Jia,Jing Zheng,Tao Duan,Qizhi He,Kai Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tbx3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (AB99302)

Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 antibody (AB99302)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (40)

Product promise