Anti-TBX3 antibody [EPR28578-38] KO Tested (ab322199)

Knockout Tested Rabbit Recombinant Monoclonal TBX3 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Dot Blot - Anti-TBX3 antibody [EPR28578-38] (AB322199), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Dot	IHC-P	WB	ICC/IF
Human	Expected	Tested	Tested	Not recommended
Mouse	Not recommended	Not recommended	Not recommended	Not recommended
Transfected cell lysate - Human	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Transfected cell lysate - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Transfected cell lysate - Human	Dilution info -	Notes -

Target data

See full target information TBX3

Function

Transcriptional repressor involved in developmental processes (PubMed:10468588). Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present in the regulatory region of several genes (PubMed:12000749). Probably plays a role in limb pattern formation (PubMed:10468588). Required for mammary placode induction, and maintenance of the mammary buds during development (By similarity). Involved in branching morphogenesis in both developing lungs and adult mammary glands, via negative modulation of target genes; acting redundantly with TBX2 (By similarity). Required, together with TBX2, to maintain cell proliferation in the embryonic lung mesenchyme; perhaps acting downstream of SHH, BMP and TGFbeta signaling (By similarity). Involved in modulating early inner ear development, acting independently of, and also redundantly with, TBX2 in different subregions of the developing ear (By similarity). Acts as a negative regulator of PML function in cellular senescence (PubMed:22002537).

Alternative names

T-box transcription factor TBX3, T-box protein 3, TBX3

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal TBX3 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28578-38
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tbx3 also known as T-box transcription factor 3 is a transcription factor belonging to the T-box gene family. It has a molecular weight of approximately 74 kDa. The protein functions by binding to specific DNA sequences and regulating gene expression. It is involved in the repression or activation of target genes. Tbx3 is expressed in many tissues including the heart developing limbs and mammary glands.

Biological function summary

T-box transcription factor 3 is significant in embryonic development and cellular differentiation. It plays an important role in the development of the heart and limbs impacting cell fate decisions. Tbx3 interacts with other T-box family members and it is part of a regulatory network controlling pluripotency and differentiation in stem cells. Its expression patterns suggest important functions in organogenesis and tissue specification.

Pathways

Tbx3 is a modulatory component in pathways controlling development and cell cycle progression. It is closely involved in the Wnt signaling and cell differentiation pathways. In the Wnt pathway Tbx3 regulates genes essential for cell proliferation and development. It interacts with proteins such as β-catenin linking it to other pathway members like LEF/TCF transcription factors further influencing cellular responses and development processes.

Associated diseases and disorders

T-box transcription factor 3 has associations with certain cancers and developmental disorders. Mutations in Tbx3 can lead to ulnar-mammary syndrome a disorder that affects limb and mammary gland development. In cancer Tbx3 overexpression is linked to melanoma progression interacting with proteins like p53 to affect cell cycle regulation and apoptotic pathways. Understanding Tbx3's role may provide insights into therapeutic targets for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Dot Blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Dot blot analysis of TBX3 using ab322199 at 1:1000 (0.506 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: 293T cells transfected with a human TBX3 expression vector containing a myc-His-tag®, whole cell lysate

Lane2: 293T cells transfected with a human TBX2 expression vector containing a myc-His-tag®, whole cell lysate
Exposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with overexpressed human TBX2 by dot blot.
In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Dot Blot - Anti-TBX3 antibody [EPR28578-38] (ab322199) at 1/1000 dilution
Lane 1: 293T cells transfected with a human TBX3 expression vector containing a myc-His-tag®, whole cell lysate
Lane 2: 293T cells transfected with a human TBX2 expression vector containing a myc-His-tag®, whole cell lysate
Secondary
All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Immunohistochemical analysis of paraffin-embedded (A) Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) cell pellet (B) TBX3 knockout HAP1 cell pellet tissue labeling TBX3 with ab322199 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on A: wild-type HAP1 cell pellet, no staining on B: TBX3 knockout HAP1 cell pellet. The section was incubated with ab322199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TBX3 with ab322199 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on human pancreas (PMID: 27632063). The section was incubated with ab322199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the bands lower than 75 kDa are unknown.
Exposure time: Lane 1: 180 seconds; Lane 2: 103 seconds.
All lanes: Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199) at 1/1000 dilution
Lane 1: Human adrenal gland tissue lysate at 20 µg
Lane 2: Human placenta tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 79 kDa
Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: HL-60.
The identity of the bands lower than 75 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 79 kDa, 36 kDa
Exposure time: 70s
Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 60 kDa is unknown.
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: 293T transfected with siRNA specifically targeting TBX3 whole cell lysate at 20 µg
Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 79 kDa, 36 kDa
Exposure time: 103s
Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 60 kDa is unknown.
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199) at 1/1000 dilution
Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg
Lane 2: TBX3 knockout HAP1 whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 79 kDa, 36 kDa
Exposure time: 103s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TBX3 with ab322199 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human placenta. The section was incubated with ab322199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling TBX3 with ab322199 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human endometrium. The section was incubated with ab322199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TBX3 with ab322199 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human breast carcinoma (PMID: 30697731). The section was incubated with ab322199 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins