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Knockout Tested Rabbit Recombinant Monoclonal TBX3 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-PWBICC/IF
Human
Expected
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Target data

Function

Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence.

Alternative names

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Knockout Tested Rabbit Recombinant Monoclonal TBX3 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28578-38

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

T-box transcription factor 3 is significant in embryonic development and cellular differentiation. It plays an important role in the development of the heart and limbs impacting cell fate decisions. Tbx3 interacts with other T-box family members and it is part of a regulatory network controlling pluripotency and differentiation in stem cells. Its expression patterns suggest important functions in organogenesis and tissue specification.

Activity summary

Tbx3 also known as T-box transcription factor 3 is a transcription factor belonging to the T-box gene family. It has a molecular weight of approximately 74 kDa. The protein functions by binding to specific DNA sequences and regulating gene expression. It is involved in the repression or activation of target genes. Tbx3 is expressed in many tissues including the heart developing limbs and mammary glands.

Pathways

Tbx3 is a modulatory component in pathways controlling development and cell cycle progression. It is closely involved in the Wnt signaling and cell differentiation pathways. In the Wnt pathway Tbx3 regulates genes essential for cell proliferation and development. It interacts with proteins such as β-catenin linking it to other pathway members like LEF/TCF transcription factors further influencing cellular responses and development processes.

Associated diseases and disorders

T-box transcription factor 3 has associations with certain cancers and developmental disorders. Mutations in Tbx3 can lead to ulnar-mammary syndrome a disorder that affects limb and mammary gland development. In cancer Tbx3 overexpression is linked to melanoma progression interacting with proteins like p53 to affect cell cycle regulation and apoptotic pathways. Understanding Tbx3's role may provide insights into therapeutic targets for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Dot Blot - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Dot Blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Dot blot analysis of TBX3 using ab322199 at 1:1000 (0.506 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

    Lane1: 293T cells transfected with a human TBX3 expression vector containing a myc-His-tag®, whole cell lysate

    Lane2: 293T cells transfected with a human TBX2 expression vector containing a myc-His-tag®, whole cell lysate

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with overexpressed human TBX2 by dot blot.

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-TBX3 antibody [EPR28578-38] (AB322199) at 1/1000 dilution

    Lane 1: 293T cells transfected with a human TBX3 expression vector containing a myc-His-tag®, whole cell lysate

    Lane 2: 293T cells transfected with a human TBX2 expression vector containing a myc-His-tag®, whole cell lysate

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Exposure time: 180s

    Dot blot analysis of TBX3 using ab322199 at 1:1000 (0.506 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

    Lane1: 293T cells transfected with a human TBX3 expression vector containing a myc-His-tag®, whole cell lysate

    Lane2: 293T cells transfected with a human TBX2 expression vector containing a myc-His-tag®, whole cell lysate

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with overexpressed human TBX2 by dot blot.

    #In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Immunohistochemical analysis of paraffin-embedded (A) Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) cell pellet (B) TBX3 knockout HAP1 cell pellet tissue labeling TBX3 with ab322199 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on A: wild-type HAP1 cell pellet, no staining on B: TBX3 knockout HAP1 cell pellet. The section was incubated with ab322199 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TBX3 with ab322199 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: no staining on human pancreas (PMID: 27632063). The section was incubated with ab322199 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands lower than 75 kDa are unknown.

    Exposure time: Lane 1: 180 seconds; Lane 2: 103 seconds.

    All lanes: Western blot - Anti-TBX3 antibody [EPR28578-38] (AB322199) at 1/1000 dilution

    Lane 1: Human adrenal gland tissue lysate at 20 µg

    Lane 2: Human placenta tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 79 kDa

  • Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HL-60.

    The identity of the bands lower than 75 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TBX3 antibody [EPR28578-38] (AB322199) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 79 kDa, 36 kDa

    Exposure time: 70s

  • Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the lower MW band at approximately 60 kDa is unknown.

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TBX3 antibody [EPR28578-38] (AB322199) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: 293T transfected with siRNA specifically targeting TBX3 whole cell lysate at 20 µg

    Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 79 kDa, 36 kDa

    Exposure time: 103s

  • Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Western blot - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the lower MW band at approximately 60 kDa is unknown.

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TBX3 antibody [EPR28578-38] (AB322199) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

    Lane 2: TBX3 knockout HAP1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 79 kDa, 36 kDa

    Exposure time: 103s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TBX3 with ab322199 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human placenta. The section was incubated with ab322199 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling TBX3 with ab322199 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human endometrium. The section was incubated with ab322199 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX3 antibody [EPR28578-38] (ab322199)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TBX3 with ab322199 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human breast carcinoma (PMID: 30697731). The section was incubated with ab322199 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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