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AB313456

Anti-Tbx3 antibody [RP23040141]

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Rabbit Recombinant Multiclonal TBX3 antibody. Suitable for ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBX3.

View Alternative Names

T-box transcription factor TBX3, T-box protein 3, TBX3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 Antibody [RP23040141] (AB313456)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tbx3 Antibody [RP23040141] (AB313456)

For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous TBX3 using ab313456 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (1 : 2000). Panel a) shows representative cells that were stained for detection and localization of TBX3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TBX3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ChIP - Anti-Tbx3 Antibody [RP23040141] (AB313456)
  • ChIP

Supplier Data

ChIP - Anti-Tbx3 Antibody [RP23040141] (AB313456)

Enrichment of endogenous TBX3 protein at specific gene loci using ab313456 : Chromatin Immunoprecipitation (ChIP) was performed using ab313456(5 ug) on sheared chromatin from 2 million HEPG2 cells. Normal Rabbit IgG (1 ug) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system with optimized PCR primer pairs for the different loci of the active P21 promoter region used as positive control target genes, and the region of the inactive MYOD, SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040141

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ChIP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TBX3.

O15119

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2.5 µg for 10^6 Cells", "ChIP-species-notes": "<p>2.5 μg x 10^6 cells</p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tbx3 also known as T-box transcription factor 3 is a transcription factor belonging to the T-box gene family. It has a molecular weight of approximately 74 kDa. The protein functions by binding to specific DNA sequences and regulating gene expression. It is involved in the repression or activation of target genes. Tbx3 is expressed in many tissues including the heart developing limbs and mammary glands.
Biological function summary

T-box transcription factor 3 is significant in embryonic development and cellular differentiation. It plays an important role in the development of the heart and limbs impacting cell fate decisions. Tbx3 interacts with other T-box family members and it is part of a regulatory network controlling pluripotency and differentiation in stem cells. Its expression patterns suggest important functions in organogenesis and tissue specification.

Pathways

Tbx3 is a modulatory component in pathways controlling development and cell cycle progression. It is closely involved in the Wnt signaling and cell differentiation pathways. In the Wnt pathway Tbx3 regulates genes essential for cell proliferation and development. It interacts with proteins such as β-catenin linking it to other pathway members like LEF/TCF transcription factors further influencing cellular responses and development processes.

T-box transcription factor 3 has associations with certain cancers and developmental disorders. Mutations in Tbx3 can lead to ulnar-mammary syndrome a disorder that affects limb and mammary gland development. In cancer Tbx3 overexpression is linked to melanoma progression interacting with proteins like p53 to affect cell cycle regulation and apoptotic pathways. Understanding Tbx3's role may provide insights into therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor involved in developmental processes (PubMed : 10468588). Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present in the regulatory region of several genes (PubMed : 12000749). Probably plays a role in limb pattern formation (PubMed : 10468588). Required for mammary placode induction, and maintenance of the mammary buds during development (By similarity). Involved in branching morphogenesis in both developing lungs and adult mammary glands, via negative modulation of target genes; acting redundantly with TBX2 (By similarity). Required, together with TBX2, to maintain cell proliferation in the embryonic lung mesenchyme; perhaps acting downstream of SHH, BMP and TGFbeta signaling (By similarity). Involved in modulating early inner ear development, acting independently of, and also redundantly with, TBX2 in different subregions of the developing ear (By similarity). Acts as a negative regulator of PML function in cellular senescence (PubMed : 22002537).
See full target information TBX3
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Product promise

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