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AB223760

Anti-TBX5 antibody

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(1 Publication)

Rabbit Polyclonal TBX5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TBX5 aa 200-400.

View Alternative Names

T-box transcription factor TBX5, T-box protein 5, TBX5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (AB223760)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (AB223760)

Paraffin embedded sections of human heart muscle tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (AB223760)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (AB223760)

Paraffin embedded sections of human lung tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (AB223760)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (AB223760)

Paraffin embedded sections of human heart pancreas tissue were stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.

Negative control : no staining observed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (AB223760)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (AB223760)

Paraffin embedded sections of human placenta tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TBX5 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

Q99593

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBX5 also known as T-box transcription factor 5 is a protein that acts as a transcription factor. It has a mass of approximately 53 kDa. TBX5 is expressed significantly in the heart and limb tissues highlighting its importance in these areas. The protein contains a T-box domain that binds to specific DNA sequences allowing it to regulate the transcription of genes involved in developmental processes. TBX5 plays a critical role during embryogenesis particularly affecting cardiac and limb development.
Biological function summary

The function of TBX5 extends to various cellular processes. It is involved in forming the cardiac septum and limb bud acting through its role as part of the transcriptional program. TBX5 doesn't typically form a complex with other proteins but interacts with partners like GATA4 to influence gene expression. These interactions enable TBX5 to activate or repress target genes necessary for organ and limb morphogenesis making it fundamental in developmental biology.

Pathways

TBX5 operates within the cardiac development and limb differentiation pathways. It interacts with other critical factors such as NKX2-5 and TBX3 to coordinate the differentiation and proliferation processes essential for forming functional heart and limb structures. In heart development TBX5 and NKX2-5 synergistically work to activate cardiac-specific genes influencing the patterning and structural formation of the heart. The protein's involvement in limb differentiation emphasizes its dual role in these important developmental pathways.

TBX5 has significant associations with conditions like Holt-Oram syndrome and congenital heart defects. Holt-Oram syndrome a disorder arising from mutations in the TBX5 gene is characterized by limb abnormalities and cardiac anomalies illustrating the protein's role in heart and limb development. TBX5 is also linked to cardiac arrhythmias with its molecular pathways involving proteins like NKX2-5. Mutations leading to TBX5 dysfunction impact these pathways resulting in developmental malformations and contributing to complex congenital conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation (PubMed : 25725155, PubMed : 25963046, PubMed : 26917986, PubMed : 27035640, PubMed : 29174768, PubMed : 8988164). Binds to the core DNA motif of NPPA promoter (PubMed : 26926761).
See full target information TBX5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:2179 PubMed35449133

2022

Golgi apparatus-targeted aggregation-induced emission luminogens for effective cancer photodynamic therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Minglun Liu,Yuncong Chen,Yan Guo,Hao Yuan,Tongxiao Cui,Shankun Yao,Suxing Jin,Huanhuan Fan,Chengjun Wang,Ran Xie,Weijiang He,Zijian Guo
View all publications
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