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Rabbit Polyclonal TBX5 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBX5 aa 200-400.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human TBX5 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99593
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation (PubMed:25725155, PubMed:25963046, PubMed:29174768, PubMed:26917986, PubMed:27035640, PubMed:8988164). Binds to the core DNA motif of NPPA promoter (PubMed:26926761).

Alternative names

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Rabbit Polyclonal TBX5 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBX5 aa 200-400.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human TBX5 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99593
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

The function of TBX5 extends to various cellular processes. It is involved in forming the cardiac septum and limb bud acting through its role as part of the transcriptional program. TBX5 doesn't typically form a complex with other proteins but interacts with partners like GATA4 to influence gene expression. These interactions enable TBX5 to activate or repress target genes necessary for organ and limb morphogenesis making it fundamental in developmental biology.

Activity summary

TBX5 also known as T-box transcription factor 5 is a protein that acts as a transcription factor. It has a mass of approximately 53 kDa. TBX5 is expressed significantly in the heart and limb tissues highlighting its importance in these areas. The protein contains a T-box domain that binds to specific DNA sequences allowing it to regulate the transcription of genes involved in developmental processes. TBX5 plays a critical role during embryogenesis particularly affecting cardiac and limb development.

Pathways

TBX5 operates within the cardiac development and limb differentiation pathways. It interacts with other critical factors such as NKX2-5 and TBX3 to coordinate the differentiation and proliferation processes essential for forming functional heart and limb structures. In heart development TBX5 and NKX2-5 synergistically work to activate cardiac-specific genes influencing the patterning and structural formation of the heart. The protein's involvement in limb differentiation emphasizes its dual role in these important developmental pathways.

Associated diseases and disorders

TBX5 has significant associations with conditions like Holt-Oram syndrome and congenital heart defects. Holt-Oram syndrome a disorder arising from mutations in the TBX5 gene is characterized by limb abnormalities and cardiac anomalies illustrating the protein's role in heart and limb development. TBX5 is also linked to cardiac arrhythmias with its molecular pathways involving proteins like NKX2-5. Mutations leading to TBX5 dysfunction impact these pathways resulting in developmental malformations and contributing to complex congenital conditions.

Product promise

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab223760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab223760)

    Paraffin embedded sections of human heart muscle tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab223760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab223760)

    Paraffin embedded sections of human lung tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab223760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab223760)

    Paraffin embedded sections of human placenta tissue stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab223760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab223760)

    Paraffin embedded sections of human heart pancreas tissue were stained for TBX5 with ab223760 at 1/50 dilution in immunohistochemical analysis.

    Negative control: no staining observed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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