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AB38883

Anti-Tbx6 antibody

4

(3 Reviews)

|

(6 Publications)

Rabbit Polyclonal TBX6 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

T-box transcription factor TBX6, T-box protein 6

1 Images
Western blot - Anti-Tbx6 antibody (AB38883)
  • WB

Project2525****

Western blot - Anti-Tbx6 antibody (AB38883)

All lanes:

Western blot - Anti-Tbx6 antibody (ab38883) at 1 µg/mL

Lane 1:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 2:

Lung (Mouse) Tissue Lysate at 10 µg

Lane 3:

Human testis tissue lysate - total protein (<a href='/en-us/products/unavailable/human-testis-tissue-lysate-total-protein-ab30257'>ab30257</a>) at 10 µg

Lane 4:

Lung (Human) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 47 kDa

Observed band size: 43 kDa,50 kDa

false

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tbx6 also known as T-box 6 is a transcription factor with a molecular mass of approximately 51 kDa. It belongs to the T-box family which is characterized by a conserved DNA-binding domain. Tbx6 is expressed in the paraxial mesoderm an area of embryonic tissue that eventually forms somites the precursors of skeletal muscle vertebrae and dermis. The expression of Tbx6 is highly regulated during developmental stages indicating its role in embryogenesis and tissue specification.
Biological function summary

Transcription factor Tbx6 plays a significant role in mesoderm specification and somite segmentation. It functions by binding to specific DNA sequences to regulate the expression of target genes essential for somite formation. Tbx6 does not form part of any known protein complex but it interacts with other transcription factors to influence mesoderm differentiation. The precise regulation of Tbx6 activity is important for proper embryonic development ensuring that the cells undertake their correct differentiation pathways.

Pathways

Tbx6 functions in pathways critical for embryonic development including the Wnt and Notch signaling pathways. The Wnt signaling pathway regulates cell fate determination while the Notch signaling controls segmentation during somitogenesis. Tbx6 interacts with proteins such as Wnt3a and RBPJ aligning its activity in these pathways to guide the formation of somites and establish anterior-posterior patterning.

Tbx6 mutations have been associated with congenital vertebral malformations and neural tube defects. These disorders arise due to the disruption of normal somitogenesis and mesoderm development often linked to faulty signaling in the Wnt and Notch pathways. Additionally protein interactions between Tbx6 and RBPJ a component of the Notch pathway highlight their collaborative role in ensuring proper vertebral column formation. Understanding Tbx6's involvement in these pathways provides insights into potential therapeutic targets for such developmental abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells : neural vs mesodermal. Acts in part by down-regulating, a specific enhancer (N1) of SOX2, to inhibit neural development. Seems to play also an essential role in left/right axis determination and acts through effects on Notch signaling around the node as well as through an effect on the morphology and motility of the nodal cilia.
See full target information Tbx6
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:2325 PubMed35484123

2022

Periodic formation of epithelial somites from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Sanaki-Matsumiya,Mitsuhiro Matsuda,Nicola Gritti,Fumio Nakaki,James Sharpe,Vikas Trivedi,Miki Ebisuya

eLife 11: PubMed35088712

2022

Paraxial mesoderm organoids model development of human somites.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph Budjan,Shichen Liu,Adrian Ranga,Senjuti Gayen,Olivier Pourquié,Sahand Hormoz

Stem cell reports 13:1083-1098 PubMed31708477

2019

Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Bredenkamp,Jian Yang,James Clarke,Giuliano Giuseppe Stirparo,Ferdinand von Meyenn,Sabine Dietmann,Duncan Baker,Rosalind Drummond,Yongming Ren,Dongwei Li,Chuman Wu,Maria Rostovskaya,Sarah Eminli-Meissner,Austin Smith,Ge Guo

Stem cell reports 11:43-57 PubMed30008328

2018

Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Ran Wang,Zhisong He,Pierre Osteil,Emilie Wilkie,Xianfa Yang,Jun Chen,Guizhong Cui,Wenke Guo,Yingying Chen,Guangdun Peng,Patrick P L Tam,Naihe Jing

Nature protocols 11:1833-50 PubMed27583644

2016

Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jérome Chal,Ziad Al Tanoury,Marie Hestin,Bénédicte Gobert,Suvi Aivio,Aurore Hick,Thomas Cherrier,Alexander P Nesmith,Kevin K Parker,Olivier Pourquié

PLoS biology 12:e1001937 PubMed25157815

2014

In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.

Applications

ICC/IF

Species

Mouse

Mina Gouti,Anestis Tsakiridis,Filip J Wymeersch,Yali Huang,Jens Kleinjung,Valerie Wilson,James Briscoe
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB209827

Anti-Tbx6 antibody

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tbx6-antibody-ab209827

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