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AB229680

Anti-TC1 antibody

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(4 Publications)

Rabbit Polyclonal TC1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC19A2.

View Alternative Names

THT1, TRMA, SLC19A2, Thiamine transporter 1, ThTr-1, ThTr1, Solute carrier family 19 member 2, Thiamine carrier 1, TC1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-TC1 antibody (AB229680)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TC1 antibody (AB229680)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TC1 (green) using ab229680 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Western blot - Anti-TC1 antibody (AB229680)
  • WB

Supplier Data

Western blot - Anti-TC1 antibody (AB229680)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-TC1 antibody (ab229680) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Western blot - Anti-TC1 antibody (AB229680)
  • WB

Supplier Data

Western blot - Anti-TC1 antibody (AB229680)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-TC1 antibody (ab229680) at 1/5000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Western blot - Anti-TC1 antibody (AB229680)
  • WB

Supplier Data

Western blot - Anti-TC1 antibody (AB229680)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-TC1 antibody (ab229680) at 1/2500 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Western blot - Anti-TC1 antibody (AB229680)
  • WB

Supplier Data

Western blot - Anti-TC1 antibody (AB229680)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-TC1 antibody (ab229680) at 1/1000 dilution

All lanes:

Rat liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLC19A2. The exact immunogen used to generate this antibody is proprietary information.

O60779

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TC1 also known as Transcriptional Coactivator 1 is a protein with a molecular mass approximately 55 kDa. It plays a role in gene expression. TC1 commonly influences transcription by interacting with transcription factors and the basal transcription machinery. The protein is mostly expressed in the nucleus of cells especially in tissues with high rates of cell proliferation like the liver and spleen. TC1 affects transcription of several genes by modifying chromatin structure and recruiting other proteins necessary for gene expression.
Biological function summary

TC1 facilitates the transcriptional activity of various genes essential for cell cycle regulation and differentiation. The protein participates in multi-protein complexes forming part of a larger assembly to enhance gene expression efficiency. Through its involvement in the regulation of cell cycle-related genes TC1 impacts cellular processes like growth proliferation and apoptosis. It modulates gene expression by restructuring chromatin to allow transcription factors and related proteins better access to DNA sequences.

Pathways

TC1 integrates into the cell cycle control and apoptosis regulation networks. It interacts with proteins such as Cyclin D playing a role in the G1/S phase transition and with members of the E2F transcription factor family which are key for DNA synthesis control. TC1's activities significantly impact pathways like the retinoblastoma (Rb) pathway which governs cell cycle progression and it aligns with proteins that regulate cell death and survival balances ensuring proper cell function and organismal homeostasis.

TC1 shows strong connections to cancer and liver disorders. Overexpression or malfunction of TC1 has been linked to increased cell proliferation and tumorigenesis especially in cancers like hepatocellular carcinoma. Aberrant TC1 activity influences Cyclin D and other cell cycle proteins contributing to uncontrolled cell division and tumor progression. Additionally irregular TC1 expression interacts with the Rb protein pathway further implicating it in the development and maintenance of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

High-affinity transporter for the intake of thiamine (PubMed : 10391222, PubMed : 10542220, PubMed : 21836059, PubMed : 33008889, PubMed : 35512554, PubMed : 35724964). Mediates H(+)-dependent pyridoxine transport (PubMed : 33008889, PubMed : 35512554, PubMed : 35724964).
See full target information SLC19A2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 56:e13477 PubMed37057399

2023

Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Chao Zhu,Wei Hu,Lei Lin,Qing-Wen Yang,Lu Zhang,Jia-Lin Xu,Yi-Tong Xu,Jia-Sheng Liu,Meng-Di Zhang,Xiao-Yu Tong,Kai-Yi Zhu,Ke Feng,Yi Feng,Jian-Zhong Su,Xiu-Feng Huang,Jin Li

American journal of physiology. Cell physiology 323:C1664-C1680 PubMed36342158

2022

Tumor necrosis factor α impedes colonic thiamin pyrophosphate and free thiamin uptake: involvement of JNK/ERK-mediated pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Selvaraj Anthonymuthu,Subrata Sabui,Alaullah Sheikh,James M Fleckenstein,Hamid M Said

The Journal of biological chemistry 298:101562 PubMed34998824

2022

Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters.

Applications

Unspecified application

Species

Unspecified reactive species

Subrata Sabui,Kalidas Ramamoorthy,Jose M Romero,Rita D Simoes,James M Fleckenstein,Hamid M Said

Canadian journal of physiology and pharmacology 100:422-431 PubMed34860610

2021

β-elemene relieves neuropathic pain in mice through the regulation on C-X-C motif chemokine receptor 3 and GABAA receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Dai,Zhenhua Zeng,Shuo Deng,Sanbao Zou,Tingyang Dou
View all publications
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