JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB96307

Anti-TC21 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal TC21 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RRAS2 aa 1-200.

View Alternative Names

TC21, RRAS2, Ras-related protein R-Ras2, Ras-like protein TC21, Teratocarcinoma oncogene

1 Images
Western blot - Anti-TC21 antibody (AB96307)
  • WB

Unknown

Western blot - Anti-TC21 antibody (AB96307)

12% SDS PAGE

All lanes:

Western blot - Anti-TC21 antibody (ab96307) at 1/5000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RRAS2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P62070

Specificity

The immunogen shares 71% homology with RRAS, P10301.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB247258

Anti-ZAP70 antibody [YE291] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [YE291] - BSA and Azide free (AB247258)

  • 0
  • 0
Primary Antibodies

AB121577

Anti-RASGRF2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

  • 0
  • 0
Primary Antibodies

AB75830

Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TC21 also known as R-Ras2 is a small GTPase with a molecular mass of approximately 23 kDa. It belongs to the Ras superfamily of proteins and plays an important role in signal transduction. TC21 switches between inactive GDP-bound and active GTP-bound states participating in the propagation of signals within the cell. The protein is expressed in a variety of tissues with significant levels found in the brain lung and lymphoid organs. Its activity is tightly regulated through interactions with guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).
Biological function summary

TC21 influences various cellular processes such as cell growth survival and differentiation. It is active in several signaling pathways contributing to the regulation of cytoskeletal organization and cell adhesion. TC21 is part of larger signaling complexes that engage in mitogenic signaling and influences the function of integrins. These activities position TC21 as a dynamic regulator of cellular behavior allowing it to coordinate and fine-tune responses to extracellular signals.

Pathways

TC21 is integral to the PI3K/AKT and MAPK signaling pathways. In these pathways TC21 interacts with related proteins like PI3K and RAF1 to transmit signals that promote cell proliferation and survival. The PI3K/AKT pathway for instance involves TC21 in the downstream activation of AKT influencing metabolism growth and apoptosis. It also connects with the MAPK pathway contributing to gene expression changes and facilitating cellular responses to growth stimuli.

Alterations in TC21 function link to oncogenic processes including breast cancer and leukemia. Overexpression or mutations in the TC21 gene can lead to abnormal signaling enhancing cell proliferation and resisting apoptosis. In these oncogenic pathways TC21 often partners with other Ras family members like HRAS which cooperate to drive tumorigenesis. Understanding TC21's role in these pathways aids in the development of targeted therapies aiming to disrupt its pathological signaling activities.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

GTP-binding protein with GTPase activity, involved in the regulation of MAPK signaling pathway and thereby controlling multiple cellular processes (PubMed : 31130282, PubMed : 31130285, PubMed : 39809765). Regulates craniofacial development (PubMed : 31130282, PubMed : 31130285).
See full target information RRAS2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 22:e51136 PubMed34369648

2021

BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters.

Applications

Unspecified application

Species

Unspecified reactive species

Ashari R Kannangara,Daniel M Poole,Colten M McEwan,Joshua C Youngs,Vajira K Weerasekara,Alex M Thornock,Misael T Lazaro,Eranga R Balasooriya,Laura M Oh,Erik J Soderblom,Jonathan J Lee,Daniel L Simmons,Joshua L Andersen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com