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AB184181

Anti-TCEA1 antibody [EPR14820(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal TCEA1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

GTF2S, TFIIS, TCEA1, Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIIS.o

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)

Immunocytochemistry analysis of a HeLa cells labeling TCEA1 with ab184181 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Dapi counterstain shown in blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)

Immunohistochemistry analysis of Human colon tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)

Immunohistochemistry analysis of Human lung adenocarcinoma tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)
  • IP

Supplier Data

Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)

Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab184181 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated used as secondary antibody at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Predicted band size: 33 kDa

false

Western blot - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)
  • WB

Supplier Data

Western blot - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)

All lanes:

Western blot - Anti-TCEA1 antibody [EPR14820(B)] (ab184181) at 1/10000 dilution

Lane 1:

K652 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

SP2/0 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

false

Western blot - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)
  • WB

Lab

Western blot - Anti-TCEA1 antibody [EPR14820(B)] (AB184181)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/100000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-TCEA1 antibody [EPR14820(B)] (ab184181) at 1/50000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) + OE-empty whole cell lysate at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) + OE-TCEA1 whole cell lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) + OE-TCEA2 whole cell lysate at 20 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) + OE-TCEA3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34-39 kDa

Observed band size: 34-39 kDa

false

Exposure time: 1s

  • Carrier free

    Anti-TCEA1 antibody [EPR14820(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14820(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCEA1 also known as transcription elongation factor A protein 1 or TFIIS plays a mechanical role in the process of transcription elongation. This protein has a molecular mass of approximately 34 kDa. TCEA1 is expressed in a wide range of tissues indicating a broad involvement in cellular functions. During transcription elongation TCEA1 interacts with RNA polymerase II helping to reactivate stalled enzyme complexes by promoting cleavage of backtracked RNA which is essential to maintain transcription fidelity.
Biological function summary

TCEA1 is important for the proper regulation and efficiency of gene expression. It is not part of a large complex but interacts specifically with RNA polymerase II to facilitate transcript elongation. Without TCEA1 cells may experience transcription arrest either temporarily or permanently which can lead to aberrant gene expression profiles. It ensures continuity in transcription so that the full-length RNA transcripts are synthesized without interruption which is necessary for correct cellular function.

Pathways

TCEA1 plays an indispensable role in the transcription machinery pathway and is closely related to components like RNA polymerase II and TFIIH within the transcription cycle. It is also connected to the DNA damage response pathways because it allows resumption of transcription after damage repair. These pathways maintain cellular homeostasis and balance gene expression to adapt to cellular needs and external signals. Together with other transcription factors TCEA1 ensures transcriptional regulation in response to cellular stimuli.

TCEA1 is implicated in transcription-related diseases such as neurodegenerative disorders and cancer. Mutations or dysregulation of TCEA1 can lead to impaired transcription which often results in cellular malfunction and disease progression. It is associated with proteins like p53 in cancer pathways where transcription arrest and restart mechanisms play a critical part in controlling cell growth and apoptosis. In neurodegenerative diseases disruption of transcriptional processes leads to neuronal cell damage and death contributing to the pathogenesis of these disorders. Understanding the role of TCEA1 in these pathways provides critical insights into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
See full target information TCEA1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International ophthalmology 44:437 PubMed39578251

2024

Carbonic anhydrase inhibitor alleviates retinal barrier toxicity in paclitaxel-induced retinopathy and macular edema by inhibiting CAXIV.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ting Ye,Ya-Li Niu,Zi-Yi Zhou,Yu Sun,Tian-Fang Chang,Yu-Tong Jing,Qian Bai,Zhao-Jie Chu
View all publications
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Product promise

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