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Rabbit Recombinant Monoclonal TCEA1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Transfected cell lysate samples. Cited in 1 publication.

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Images

Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (AB185947), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (AB185947), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (AB185947), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (AB185947), expandable thumbnail
  • Immunoprecipitation - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (AB185947), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Expected
Rat
Predicted
Predicted
Predicted
Predicted
Predicted
Transfected cell lysate
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30 - 1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Transfected cell lysate
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Additional Targets

TCEA2, TCEA3

Alternative names

GTF2S, TFIIS, TCEA1, Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIIS.o

Recommended products

Rabbit Recombinant Monoclonal TCEA1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Transfected cell lysate samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14821
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TCEA1 also known as transcription elongation factor A protein 1 or TFIIS plays a mechanical role in the process of transcription elongation. This protein has a molecular mass of approximately 34 kDa. TCEA1 is expressed in a wide range of tissues indicating a broad involvement in cellular functions. During transcription elongation TCEA1 interacts with RNA polymerase II helping to reactivate stalled enzyme complexes by promoting cleavage of backtracked RNA which is essential to maintain transcription fidelity.

Biological function summary

TCEA1 is important for the proper regulation and efficiency of gene expression. It is not part of a large complex but interacts specifically with RNA polymerase II to facilitate transcript elongation. Without TCEA1 cells may experience transcription arrest either temporarily or permanently which can lead to aberrant gene expression profiles. It ensures continuity in transcription so that the full-length RNA transcripts are synthesized without interruption which is necessary for correct cellular function.

Pathways

TCEA1 plays an indispensable role in the transcription machinery pathway and is closely related to components like RNA polymerase II and TFIIH within the transcription cycle. It is also connected to the DNA damage response pathways because it allows resumption of transcription after damage repair. These pathways maintain cellular homeostasis and balance gene expression to adapt to cellular needs and external signals. Together with other transcription factors TCEA1 ensures transcriptional regulation in response to cellular stimuli.

Associated diseases and disorders

TCEA1 is implicated in transcription-related diseases such as neurodegenerative disorders and cancer. Mutations or dysregulation of TCEA1 can lead to impaired transcription which often results in cellular malfunction and disease progression. It is associated with proteins like p53 in cancer pathways where transcription arrest and restart mechanisms play a critical part in controlling cell growth and apoptosis. In neurodegenerative diseases disruption of transcriptional processes leads to neuronal cell damage and death contributing to the pathogenesis of these disorders. Understanding the role of TCEA1 in these pathways provides critical insights into potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947), expandable thumbnail

    Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947)

    All lanes: Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947) at 1/10000 dilution

    Lane 1: K562 cell lysate at 20 µg

    Lane 2: HeLa cell lysate at 20 µg

    Lane 3: SP2/0 cell lysate at 20 µg

    Lane 4: HUVEC cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947)

    Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/500 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947)

    Immunofluorescent staining of -20 °C acetone fixed HeLa cells labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/500 dilution (red; left image) and DAPI nuclear staining (blue; right image). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947)

    Immunofluorescent staining of 4% paraformaldehyde fixed HUVEC cells labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/500 dilution (red; left image) and DAPI nuclear staining (blue; right image). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.

  • Immunoprecipitation - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947), expandable thumbnail

    Immunoprecipitation - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947)

    Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/50 dilution. Secondary staining with Goat anti Rabbit IgG (H+L) Peroxidase conjugated antibody at a 1/1000 dilution.

    All lanes: Immunoprecipitation - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947)

    Predicted band size: 33 kDa

  • Flow Cytometry (Intracellular) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947)

    Intracellular flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/250 dilution (red) compared to a Rabbit monoclonal IgG isotype control antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC) at a 1/150 dilution.

  • Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947), expandable thumbnail

    Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.
    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    All lanes: Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (ab185947) at 1/10000 dilution

    Lane 1: 293T (Human embryonic kidney epithelial cell) + OE-empty whole cell lysate at 20 µg

    Lane 2: 293T (Human embryonic kidney epithelial cell) + OE-TCEA1 whole cell lysate at 20 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) + OE-TCEA2 whole cell lysate at 20 µg

    Lane 4: 293T (Human embryonic kidney epithelial cell) + OE-TCEA3 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 34-39 kDa

    Observed band size: 34-39 kDa

    Exposure time: 1s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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