AB242405

Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TCEA1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Transfected cell lysate samples. Cited in 1 publication.

View Alternative Names

GTF2S, TFIIS, TCEA1, Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIIS.o

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Immunofluorescent staining of 4% paraformaldehyde fixed HUVEC cells labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/500 dilution (red; left image) and DAPI nuclear staining (blue; right image). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor^® 555) at a 1/200 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/500 dilution. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Intracellular flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/250 dilution (red) compared to a Rabbit monoclonal IgG isotype control antibody (green). Secondary staining with Goat anti Rabbit IgG (FITC) at a 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Immunofluorescent staining of -20 °C acetone fixed HeLa cells labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/500 dilution (red; left image) and DAPI nuclear staining (blue; right image). Secondary staining with Goat anti Rabbit IgG (Alexa Fluor^® 555) at a 1/200 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)

Supplier Data

Immunoprecipitation - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 + TCEA2 + TCEA3 with ab185947 at 1/50 dilution. Secondary staining with Goat anti Rabbit IgG (H+L) Peroxidase conjugated antibody at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)

All lanes:

Immunoprecipitation - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (<a href='/en-us/products/primary-antibodies/tcea1-tcea2-tcea3-antibody-epr14821-ab185947'>ab185947</a>)

Predicted band size: 33 kDa

false

Lab

Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947) Blocking and diluting buffer and concentration : 5% NFDM /TBST. ab181602 was used as a GAPDH loading control.

All lanes:

Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] (<a href='/en-us/products/primary-antibodies/tcea1-tcea2-tcea3-antibody-epr14821-ab185947'>ab185947</a>) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) + OE-empty whole cell lysate at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) + OE-TCEA1 whole cell lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) + OE-TCEA2 whole cell lysate at 20 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) + OE-TCEA3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34-39 kDa

Observed band size: 34-39 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14821

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt (Intra), WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab242405 is the carrier-free version of ab185947.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCEA1 also known as transcription elongation factor A protein 1 or TFIIS plays a mechanical role in the process of transcription elongation. This protein has a molecular mass of approximately 34 kDa. TCEA1 is expressed in a wide range of tissues indicating a broad involvement in cellular functions. During transcription elongation TCEA1 interacts with RNA polymerase II helping to reactivate stalled enzyme complexes by promoting cleavage of backtracked RNA which is essential to maintain transcription fidelity.

Biological function summary

TCEA1 is important for the proper regulation and efficiency of gene expression. It is not part of a large complex but interacts specifically with RNA polymerase II to facilitate transcript elongation. Without TCEA1 cells may experience transcription arrest either temporarily or permanently which can lead to aberrant gene expression profiles. It ensures continuity in transcription so that the full-length RNA transcripts are synthesized without interruption which is necessary for correct cellular function.

Pathways

TCEA1 plays an indispensable role in the transcription machinery pathway and is closely related to components like RNA polymerase II and TFIIH within the transcription cycle. It is also connected to the DNA damage response pathways because it allows resumption of transcription after damage repair. These pathways maintain cellular homeostasis and balance gene expression to adapt to cellular needs and external signals. Together with other transcription factors TCEA1 ensures transcriptional regulation in response to cellular stimuli.

TCEA1 is implicated in transcription-related diseases such as neurodegenerative disorders and cancer. Mutations or dysregulation of TCEA1 can lead to impaired transcription which often results in cellular malfunction and disease progression. It is associated with proteins like p53 in cancer pathways where transcription arrest and restart mechanisms play a critical part in controlling cell growth and apoptosis. In neurodegenerative diseases disruption of transcriptional processes leads to neuronal cell damage and death contributing to the pathogenesis of these disorders. Understanding the role of TCEA1 in these pathways provides critical insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

See full target information TCEA1

Additional targets

TCEA2,TCEA3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 19:97 PubMed39696466

2024

The UFMylation pathway is impaired in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tingxiang Yan,Michael G Heckman,Emily C Craver,Chia-Chen Liu,Bailey D Rawlinson,Xue Wang,Melissa E Murray,Dennis W Dickson,Nilufer Ertekin-Taner,Zhenkun Lou,Guojun Bu,Wolfdieter Springer,Fabienne C Fiesel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Flow Cytometry (Intracellular) - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Immunoprecipitation - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Western blot - Anti-TCEA1 + TCEA2 + TCEA3 antibody [EPR14821] - BSA and Azide free (AB242405)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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