Rabbit Polyclonal TCF-25 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human TCF25.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TCF25. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BQ70
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed:30244831). In the RQC complex, required to promote formation of 'Lys-48'-linked polyubiquitin chains during ubiquitination of incompletely synthesized proteins by LTN1 (PubMed:30244831). Also acts as a transcriptional repressor: represses transcription of SRF in vitro and so may play a role in heart development (PubMed:16574069). May play a role in cell death control (By similarity).
Alternative names
KIAA1049, NULP1, FKSG26, TCF25, Ribosome quality control complex subunit TCF25, Nuclear localized protein 1, Transcription factor 25, TCF-25
Recommended products
Rabbit Polyclonal TCF-25 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human TCF25.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TCF25. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BQ70
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCF-25 also known as Transcription Factor 25 plays an important role in gene expression by regulating transcription processes. Its protein mass is approximately 25 kDa. TCF-25 is expressed broadly across several tissues with particular prominence in the brain and heart. This expression pattern suggests its involvement in critical cellular processes in these organ systems.
- Biological function summary
TCF-25 modulates expression of genes involved in development and differentiation. It functions as a part of a complex with other transcription factors to regulate target genes. This complex interaction enhances its ability to control biological processes maintaining cellular homeostasis and supporting the progression of various developmental stages.
- Pathways
Studies show TCF-25 plays an influential role in both Wnt and Notch signaling pathways. These pathways are important for cell fate determination and tissue development. Within these pathways TCF-25 interacts with proteins such as beta-catenin in the Wnt signaling pathway and works alongside Notch receptors in the Notch signaling pathway illustrating its integration into these significant cellular signaling networks.
- Associated diseases and disorders
Research finds TCF-25 linked to cardiovascular diseases and neuronal development disorders. Abnormalities in TCF-25 expression or function contribute to pathogenesis in conditions like congenital heart defects where its partnership with proteins like GATA4 and NKX2-5 is essential. Additionally misregulation of TCF-25 can impact neural processes potentially leading to neurological disorders through its interactions with other transcription factors.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-TCF-25 antibody (ab227150)
7.5% SDS PAGE
All lanes: Western blot - Anti-TCF-25 antibody (ab227150) at 1/1000 dilution
All lanes: Mouse heart tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
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