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Mouse Monoclonal TCF-25 antibody. Suitable for Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TCF25.

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Images

Flow Cytometry - Anti-TCF-25 antibody [PCRP-TCF25-1A11] (AB277101), expandable thumbnail
  • Protein Array - Anti-TCF-25 antibody [PCRP-TCF25-1A11] (AB277101), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human TCF25. Database link Q9BQ70

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytProtein Array
Human
Tested
Expected
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Recombinant full length protein - Human
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg for 106 Cells

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed:30244831). In the RQC complex, required to promote formation of 'Lys-48'-linked polyubiquitin chains during ubiquitination of incompletely synthesized proteins by LTN1 (PubMed:30244831). Also acts as a transcriptional repressor: represses transcription of SRF in vitro and so may play a role in heart development (PubMed:16574069). May play a role in cell death control (By similarity).

Alternative names

Recommended products

Mouse Monoclonal TCF-25 antibody. Suitable for Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TCF25.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human TCF25. Database link Q9BQ70
Clone number

PCRP-TCF25-1A11

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from bioreactor concentrate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TCF-25 also known as Transcription Factor 25 plays an important role in gene expression by regulating transcription processes. Its protein mass is approximately 25 kDa. TCF-25 is expressed broadly across several tissues with particular prominence in the brain and heart. This expression pattern suggests its involvement in critical cellular processes in these organ systems.

Biological function summary

TCF-25 modulates expression of genes involved in development and differentiation. It functions as a part of a complex with other transcription factors to regulate target genes. This complex interaction enhances its ability to control biological processes maintaining cellular homeostasis and supporting the progression of various developmental stages.

Pathways

Studies show TCF-25 plays an influential role in both Wnt and Notch signaling pathways. These pathways are important for cell fate determination and tissue development. Within these pathways TCF-25 interacts with proteins such as beta-catenin in the Wnt signaling pathway and works alongside Notch receptors in the Notch signaling pathway illustrating its integration into these significant cellular signaling networks.

Associated diseases and disorders

Research finds TCF-25 linked to cardiovascular diseases and neuronal development disorders. Abnormalities in TCF-25 expression or function contribute to pathogenesis in conditions like congenital heart defects where its partnership with proteins like GATA4 and NKX2-5 is essential. Additionally misregulation of TCF-25 can impact neural processes potentially leading to neurological disorders through its interactions with other transcription factors.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-TCF-25 antibody [PCRP-TCF25-1A11] (ab277101), expandable thumbnail

    Flow Cytometry - Anti-TCF-25 antibody [PCRP-TCF25-1A11] (ab277101)

    Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TCF-25 using ab277101 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

  • Protein Array - Anti-TCF-25 antibody [PCRP-TCF25-1A11] (ab277101), expandable thumbnail

    Protein Array - Anti-TCF-25 antibody [PCRP-TCF25-1A11] (ab277101)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277101.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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