Rabbit Polyclonal TCF-4/TCF7L2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.
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Publications
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- Journal of cellular physiology 237:2877-28872022Role of the hydrogen sulfide-releasing donor ADT-OH in the regulation of mammal neural precursor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan-Wen Wei,Ming-Ming Zou,Jian Huan,Di Li,Peng-Fei Zhang,Mei-Hong Lu,Jian Xiong,Yan-Xia MaPubMed 35342944
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e221852022TCF7L2 transcriptionally regulates Fgf15 to maintain bile acid and lipid homeostasis through gut-liver crosstalk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeha Bhat,Fatemehsadat Esteghamat,Bal Krishna Chaube,Kushan Gunawardhana,Mitra Mani,Clay Thames,Dhanpat Jain,Henry N Ginsberg,Carlos Fernandes-Hernando,Arya ManiPubMed 35133032
- Frontiers in pharmacology 12:6827132021Ginsenoside Rb1 Lessens Gastric Precancerous Lesions by Interfering With β-Catenin/TCF4 Interaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhao Zeng,Xiao Ma,Ziyi Zhao,Yu Chen,Jundong Wang,Yanwei Hao,Junrong Yu,Zhongzhen Zeng,Nianzhi Chen,Maoyuan Zhao,Jianyuan Tang,Daoyin GongPubMed 34594214
- American journal of physiology. Gastrointestinal a 307:G1044-562014SIM2 maintains innate host defense of the small intestine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuan-Jung Chen et. al.PubMed 25277798
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Target data
Function
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
Alternative names
TCF4, TCF7L2, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4
Recommended products
Rabbit Polyclonal TCF-4/TCF7L2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCF-4 also known as TCF7L2 is a transcription factor that plays an important mechanical role in the Wnt signaling pathway. It is a protein with a molecular mass of approximately 66 kDa and is expressed in many tissues including the intestine pancreas and brain. TCF-4 acts by binding to DNA at specific sites and regulating the transcription of target genes. This transcription regulation occurs when TCF-4 partners with beta-catenin altering gene expression in response to Wnt signals.
- Biological function summary
TCF-4 influences processes like cell proliferation differentiation and apoptosis. It functions as part of a transcriptional complex with members such as beta-catenin and other cofactors which are essential for its regulatory activities. Through its control of gene expression TCF-4 affects tissue homeostasis and has implications in embryonic development. It modulates the expression of genes that are involved in important cellular processes making it a significant player in maintaining cellular equilibrium.
- Pathways
TCF-4 is essential in the Wnt/beta-catenin signaling pathway a critical pathway that regulates cell fate and patterning during development. This pathway involves the accumulation of beta-catenin in the cytoplasm and its subsequent translocation into the nucleus. In the nucleus beta-catenin partners with TCF-4 to regulate gene transcription. Additionally TCF-4 interacts with other pathway proteins such as LEF1 (Lymphoid Enhancer-Binding Factor 1) to modulate Wnt target genes illustrating its integration within complex signaling networks.
- Associated diseases and disorders
TCF-4 has strong connections to type 2 diabetes and colorectal cancer. Variations in the TCF7L2 gene have been linked to increased risk of type 2 diabetes potentially due to its role in glucose metabolism. In colorectal cancer aberrant activation of the Wnt signaling pathway often involving TCF-4 and beta-catenin leads to uncontrolled cell growth. Understanding these connections can provide insights into the pathogenesis of these diseases and inform treatment strategies targeting TCF-4 and its associated pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF-4/TCF7L2 antibody (ab134275)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TCF-4/TCF7L2 with ab134275 at 5μg/ml. Primary incubation was followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-TCF-4/TCF7L2 antibody (ab134275)
All lanes: Western blot - Anti-TCF-4/TCF7L2 antibody (ab134275) at 1/500 dilution
All lanes: HeLa cell lysate
Predicted band size: 68 kDa
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