Rabbit Recombinant Monoclonal TCF-4/TCF7L2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-TCF-4/TCF7L2 antibody [EPR24109-96] - BSA and Azide free (Detector) (AB285147), expandable thumbnail](https://content.abcam.com/products/images/tcf-4-tcf7l2-antibody-epr24109-96-bsa-and-azide-free-detector-ab285147--sandwich-elisa-img409183.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-TCF-4/TCF7L2 antibody [EPR24109-96] - BSA and Azide free (Detector) (AB285147)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
Alternative names
TCF4, TCF7L2, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4
Recommended products
Rabbit Recombinant Monoclonal TCF-4/TCF7L2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24109-96
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab285147 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TCF-4/TCF7L2. The recommended pair for sandwich ELISA is:
Capture: Anti-TCF-4/TCF7L2 antibody [EPR24109-42] - BSA and Azide free (Capture) ab285041, Human TCF-4/TCF7L2 Capture Antibody (unconjugated)
Detector: ab285147, Human TCF-4/TCF7L2 Detector Antibody (unconjugated)
The reference range value is 31.25 - 4000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCF-4 also known as TCF7L2 is a transcription factor that plays an important mechanical role in the Wnt signaling pathway. It is a protein with a molecular mass of approximately 66 kDa and is expressed in many tissues including the intestine pancreas and brain. TCF-4 acts by binding to DNA at specific sites and regulating the transcription of target genes. This transcription regulation occurs when TCF-4 partners with beta-catenin altering gene expression in response to Wnt signals.
- Biological function summary
TCF-4 influences processes like cell proliferation differentiation and apoptosis. It functions as part of a transcriptional complex with members such as beta-catenin and other cofactors which are essential for its regulatory activities. Through its control of gene expression TCF-4 affects tissue homeostasis and has implications in embryonic development. It modulates the expression of genes that are involved in important cellular processes making it a significant player in maintaining cellular equilibrium.
- Pathways
TCF-4 is essential in the Wnt/beta-catenin signaling pathway a critical pathway that regulates cell fate and patterning during development. This pathway involves the accumulation of beta-catenin in the cytoplasm and its subsequent translocation into the nucleus. In the nucleus beta-catenin partners with TCF-4 to regulate gene transcription. Additionally TCF-4 interacts with other pathway proteins such as LEF1 (Lymphoid Enhancer-Binding Factor 1) to modulate Wnt target genes illustrating its integration within complex signaling networks.
- Associated diseases and disorders
TCF-4 has strong connections to type 2 diabetes and colorectal cancer. Variations in the TCF7L2 gene have been linked to increased risk of type 2 diabetes potentially due to its role in glucose metabolism. In colorectal cancer aberrant activation of the Wnt signaling pathway often involving TCF-4 and beta-catenin leads to uncontrolled cell growth. Understanding these connections can provide insights into the pathogenesis of these diseases and inform treatment strategies targeting TCF-4 and its associated pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-TCF-4/TCF7L2 antibody [EPR24109-96] - BSA and Azide free (Detector) (ab285147), expandable thumbnail](https://content.abcam.com/products/images/tcf-4-tcf7l2-antibody-epr24109-96-bsa-and-azide-free-detector-ab285147--sandwich-elisa-img409183.jpg "Sandwich ELISA - Anti-TCF-4/TCF7L2 antibody [EPR24109-96] - BSA and Azide free (Detector) (ab285147)")
Sandwich ELISA - Anti-TCF-4/TCF7L2 antibody [EPR24109-96] - BSA and Azide free (Detector) (ab285147)
Example of Human TCF-4/TCF7L2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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