AB272235

Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87]

20ul selling size
RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal TCF-4/TCF7L2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

Tcf4, Tcf7l2, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, mTCF-4, Tcf3, Tcf7l1, Transcription factor 7-like 1, HMG box transcription factor 3, TCF-3, mTCF-3

5 Images

Lab

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 17003360)

Exposure time : Lane 1-6 : 15 seconds ; Lane 7-9 : 37 seconds

All lanes:

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (ab272235) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 60 µg

Lane 2:

Human kidney tissue lysate at 60 µg

Lane 3:

Human skeletal muscle tissue lysate at 60 µg

Lane 4:

Mouse liver tissue lysate at 60 µg

Lane 5:

Mouse kidney tissue lysate at 60 µg

Lane 6:

Mouse skeletal muscle tissue lysate at 60 µg

Lane 7:

Rat liver tissue lysate at 60 µg

Lane 8:

Rat kidney tissue lysate at 60 µg

Lane 9:

Rat skeletal muscle tissue lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 22945304).

Exposure time : Lane 1-2 : 3 minutes ; Lane 3-4 : 48 seconds

All lanes:

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (ab272235) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 60 µg

Lane 2:

SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 60 µg

Lane 3:

Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 60 µg

Lane 4:

L6 (rat skeletal muscle myoblast), whole cell lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature. The 75 kDa band is the long isoform of TCF7L2, while the 55 kDa band is the short isoform of TCF7L2 (PMID : 22945304, PMID : 29317436).

Exposure time : 15 seconds

All lanes:

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (ab272235) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID : 29317436).

Exposure time : 48 seconds

All lanes:

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (ab272235) at 1/1000 dilution

Lane 1:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 60 µg

Lane 2:

3T3-L1 differentiation for 6 days whole cell lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody has cross-reaction with mouse TCFL7L1.

The recombinant proteins TCFL7L1 and TCFL7L2 were made in-house.

The sample loaded onto lane 1 was E.coli extracts containing TCFL7L1 recombinant protein.

The sample loaded onto lanes 2 was purified TCF7L2 recombinant protein expressed from a mammalian - HEK-293 expression system.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (ab272235) at 1/1000 dilution

Lane 1:

Mouse TCF7L1 recombinant protein at 0.01 µg

Lane 2:

Mouse TCF7L2 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

false

Carrier free

Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24701-87

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCF-4/TCF7L2 and TCF-3/TCF7L1 proteins are part of the T-cell factor/lymphoid enhancer-binding factor (TCF/LEF) family. These proteins also known as transcription factor 7 like 2 and transcription factor 7 like 1 function as transcription factors in the Wnt signaling pathway. TCF7L2 and TCF7L1 typically weigh around 65 kDa and 56 kDa respectively. Expression of these proteins occurs in many tissues notably in the colon liver and pancreas. Mechanically they interact with β-catenin to regulate the transcription of Wnt target genes.

Biological function summary

TCF-4/TCF7L2 and TCF-3/TCF7L1 play key roles in controlling gene expression essential for cell proliferation differentiation and embryonic development. These transcription factors often form complexes with β-catenin coactivators or corepressors to modulate gene transcription activity. By influencing genetic programs they contribute to developmental processes and the maintenance of stem cells' properties. Their involvement in embryogenesis highlights their importance in developmental signaling.

Pathways

TCF-4/TCF7L2 and TCF-3/TCF7L1 are significant in the canonical Wnt/β-catenin signaling pathway. This pathway influences many cellular processes including cell fate and cellular proliferation decisions. When Wnt ligands activate the pathway β-catenin accumulates in the cytoplasm and enters the nucleus where it partners with TCF proteins to modify gene expression. This interaction highlights the relationship of TCF proteins with β-catenin and other Wnt-associated elements like Axin and APC which regulate β-catenin stability.

Alterations in TCF7L2 and TCF7L1 activity impact conditions like colorectal cancer and type 2 diabetes. Mutations or misexpression of TCF7L2 show strong links to increased risk of type 2 diabetes across various populations. In colorectal cancer changes in Wnt signaling and TCF's role as transcription factors contribute to oncogenesis. TCF proteins interact with other factors in these contexts such as the adenomatous polyposis coli (APC) protein highlighting their relevance in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif CCTTTGATC in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1 (By similarity). Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.

See full target information Tcf7l2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 14:e1682 PubMed38769659

2024

Three-dimensional chromatin landscapes in somatotroph tumour.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Guo,Yiyuan Chen,Haibo Zhu,Xinyu Tong,Lei Cao,Yazhuo Zhang,Weiyan Xie,Chuzhong Li

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22885 PubMed37000492

2023

Protective mechanism of TCF7L1 against retinal photoreceptor cell injury following retinitis pigmentosa based on the GEO database.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yin,Shuai Wu,Lingzhi Niu,Shiwei Huang

Disease markers 2022:5045873 PubMed35845134

2022

Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying MicroRNA-29a Improves Ovarian Function of Mice with Primary Ovarian Insufficiency by Targeting HMG-Box Transcription Factor/Wnt/-Catenin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Gao,Yi Cao,Min Hu,Ying Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87]

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise