AB314082

Anti-TCF1/TCF7 antibody [BLR228K] - BSA free

Recombinant
KO Validated
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Rabbit Recombinant Monoclonal TCF7 antibody. Suitable for ICC, IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TCF7 aa 200-250.

View Alternative Names

TCF1, TCF7, Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Detection of human TCF1/TCF7 by immunohistochemistry. Sample : FFPE section of human lymph node. Antibody : Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF). Secondary : HRP-conjugated goat antirabbit IgG.

Supplier Data

Immunocytochemistry - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Detection of human TCF1/TCF7 by immunocytochemistry. Sample : FFPE sections of MOLT4 cells (left) and MOLT4 knock-out cells (right). Antibody : Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Immunocytochemistry - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Detection of mouse TCF1/TCF7 by immunocytochemistry Sample : FFPE section of EL4 cells. Antibody : Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Detection of mouse TCF1/TCF7 by immunohistochemistry. Sample : FFPE section of mouse spleen. Antibody : Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Samples : Whole cell lysate (50 µg) prepared using NETN lysis buffer from MOLT4 cells (WT and KO). Antibody : Rabbit antiTCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (ab314082) at 1/1000 dilution

Lane 1:

MOLT4 WT Whole cell lysate at 50 µg

Lane 2:

MOLT4 KO Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Samples : Whole cell lysate (50 µg) from HeLa, Jurkat, HEK293T, MOLT-4, and U2OS cells prepared using NETN lysis buffer. Antibody : Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (ab314082) at 1/1000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

Jurkat Whole cell lysate at 50 µg

Lane 3:

HEK293T Whole cell lysate at 50 µg

Lane 4:

MOLT-4 Whole cell lysate at 50 µg

Lane 5:

U2OS Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Samples : Whole cell lysate (50 µg) from EL4, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (ab314082) at 1/1000 dilution

Lane 1:

EL4 Whole cell lysate at 50 µg

Lane 2:

TCMK-1 Whole cell lysate at 50 µg

Lane 3:

BW5147 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR228K

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P, ICC

applications

Immunogen

Synthetic Peptide within Human TCF7 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P36402

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCF7 also known as TCF1 is a transcription factor belonging to the TCF/LEF family with a molecular mass of approximately 54 kDa. TCF7 plays a significant mechanical role in binding to DNA and regulating gene expression in cell nuclei. It interacts with beta-catenin to control the transcription of target genes. This protein is expressed in various tissues including the thymus and other parts of the immune system as well as the intestines.

Biological function summary

TCF7 regulates cell fate decisions and differentiation specifically in the T-cell lineage. It is part of a protein complex with beta-catenin and acts as a transcriptional activator. TCF7 influences the development and function of T-cells playing a critical role in adaptive immune response. It contributes to the maintenance of stem cell properties in various tissues impacting processes like cell proliferation and survival.

Pathways

TCF7 is a vital component of the Wnt/beta-catenin signaling pathway which is essential for regulating gene expression in various cellular processes. This pathway maintains homeostasis in cell proliferation differentiation and stem cell renewal. Within this signaling network beta-catenin an important mediator partners with TCF7 to modulate target gene transcription. TCF7 also interacts with other proteins such as LEF1 which similarly participates in the transcription regulation vital for cellular development.

TCF7 associates with several pathological conditions including cancers and immunodeficiencies. Abnormal activation of the Wnt/beta-catenin pathway involving TCF7 contributes to the development of colon cancer by promoting unregulated cell division. TCF7 mutations have been linked to immunodeficiency disorders given its critical role in T-cell development. These mutations may disrupt normal T-cell formation leading to impaired immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.

See full target information TCF7

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCF1/TCF7 antibody [BLR228K] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Immunocytochemistry - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Immunocytochemistry - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Western blot - Anti-TCF1/TCF7 antibody [BLR228K] - BSA free (AB314082)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Cell Lines & Lysates

Product promise