Rabbit Recombinant Monoclonal TCF7 antibody. Suitable for ICC, IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TCF7 aa 200-250.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
ICC | IHC-P | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.
TCF1, TCF7, TCF1, Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1
Rabbit Recombinant Monoclonal TCF7 antibody. Suitable for ICC, IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TCF7 aa 200-250.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
BLR228K
Blue Ice
Up to 12 months
+4°C
+4°C
This supplementary information is collated from multiple sources and compiled automatically.
TCF7 also known as TCF1 is a transcription factor belonging to the TCF/LEF family with a molecular mass of approximately 54 kDa. TCF7 plays a significant mechanical role in binding to DNA and regulating gene expression in cell nuclei. It interacts with beta-catenin to control the transcription of target genes. This protein is expressed in various tissues including the thymus and other parts of the immune system as well as the intestines.
TCF7 regulates cell fate decisions and differentiation specifically in the T-cell lineage. It is part of a protein complex with beta-catenin and acts as a transcriptional activator. TCF7 influences the development and function of T-cells playing a critical role in adaptive immune response. It contributes to the maintenance of stem cell properties in various tissues impacting processes like cell proliferation and survival.
TCF7 is a vital component of the Wnt/beta-catenin signaling pathway which is essential for regulating gene expression in various cellular processes. This pathway maintains homeostasis in cell proliferation differentiation and stem cell renewal. Within this signaling network beta-catenin an important mediator partners with TCF7 to modulate target gene transcription. TCF7 also interacts with other proteins such as LEF1 which similarly participates in the transcription regulation vital for cellular development.
TCF7 associates with several pathological conditions including cancers and immunodeficiencies. Abnormal activation of the Wnt/beta-catenin pathway involving TCF7 contributes to the development of colon cancer by promoting unregulated cell division. TCF7 mutations have been linked to immunodeficiency disorders given its critical role in T-cell development. These mutations may disrupt normal T-cell formation leading to impaired immune responses.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Samples: Whole cell lysate (50 µg) from EL4, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-TCF1/TCF7 antibody [BLR228K] (ab314082) at 1/1000 dilution
Lane 1: EL4 Whole cell lysate at 50 µg
Lane 2: TCMK-1 Whole cell lysate at 50 µg
Lane 3: BW5147 Whole cell lysate at 50 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Samples: Whole cell lysate (50 µg) prepared using NETN lysis buffer from MOLT4 cells (WT and KO). Antibody: Rabbit antiTCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-TCF1/TCF7 antibody [BLR228K] (ab314082) at 1/1000 dilution
Lane 1: MOLT4 WT Whole cell lysate at 50 µg
Lane 2: MOLT4 KO Whole cell lysate at 50 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Samples: Whole cell lysate (50 µg) from HeLa, Jurkat, HEK293T, MOLT-4, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-TCF1/TCF7 antibody [BLR228K] (ab314082) at 1/1000 dilution
Lane 1: HeLa Whole cell lysate at 50 µg
Lane 2: Jurkat Whole cell lysate at 50 µg
Lane 3: HEK293T Whole cell lysate at 50 µg
Lane 4: MOLT-4 Whole cell lysate at 50 µg
Lane 5: U2OS Whole cell lysate at 50 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Detection of mouse TCF1/TCF7 by immunocytochemistry Sample: FFPE section of EL4 cells. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human TCF1/TCF7 by immunocytochemistry. Sample: FFPE sections of MOLT4 cells (left) and MOLT4 knock-out cells (right). Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human TCF1/TCF7 by immunohistochemistry. Sample: FFPE section of human lymph node. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF). Secondary: HRP-conjugated goat antirabbit IgG.
Detection of mouse TCF1/TCF7 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody [BLR228K] (A700-228CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
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