AB252269

Anti-TCF12 antibody [7B3] - BSA and Azide free

Recombinant
KO Validated
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Rat Recombinant Monoclonal TCF12 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

View Alternative Names

BHLHB20, HEB, HTF4, TCF12, Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4, bHLHb20

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Supplier Data

Western blot - Anti-TCF12 antibody [7B3] - BSA and Azide free (AB252269)

The expression molecular weight observed is consistent with what has been described in the literature (PMID : 16785505).

Blocking/diluting buffer and concentration : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243149).

All lanes:

Western blot - Anti-TCF12 antibody [7B3] (<a href='/en-us/products/primary-antibodies/tcf12-antibody-7b3-ab243149'>ab243149</a>) at 0.394 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 5:

WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 72 kDa

Observed band size: 92 kDa

false

Unconjugated

Anti-TCF12 antibody [7B3]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7B3

Isotype

IgG2b

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab252269 is the carrier-free version of ab243149.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCF12 also known as transcription factor 12 or Heb functions as a basic helix-loop-helix (bHLH) transcription factor. It plays roles in DNA binding and regulation of gene expression. The protein has a molecular mass of approximately 75 kDa. TCF12 expresses in various tissues with notable levels observed in the brain and skeletal muscle. It influences the development and differentiation of different cell types due to its transcriptional regulation activities.

Biological function summary

TCF12 controls the transcription of genes involved in cellular differentiation proliferation and development. It frequently functions as part of heterodimeric complexes with other bHLH proteins such as E2A. These complexes allow TCF12 to regulate a variety of target genes involved in tissue development and cell fate decisions. TCF12's ability to form these complexes enhances its functional diversity and impact on biological processes.

Pathways

The role of TCF12 extends to the Notch signaling pathway where it interacts with proteins like Notch receptors to affect cell fate determination. The protein also participates in the Myogenesis pathway collaborating with MyoD to facilitate the differentiation of myoblasts into mature muscle fibers. These pathways highlight TCF12's involvement in developmental processes and lineage specification.

TCF12 mutations and dysregulation have associations with craniosynostosis a condition characterized by premature fusion of cranial sutures. TCF12 also relates to certain types of cancer due to its regulatory effects on cell proliferation. In these contexts TCF12 may interact with proteins like TWIST1 which also influences developmental and tumorigenic processes highlighting potential therapeutic targets for intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (By similarity). May be involved in the functional network that regulates the development of the GnRH axis (PubMed : 32620954).

See full target information TCF12

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCF12 antibody [7B3] - BSA and Azide free

Western blot - Anti-TCF12 antibody [7B3] - BSA and Azide free (AB252269)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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