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AB222040

Anti-TCF12 antibody

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(1 Publication)

Rabbit Polyclonal TCF12 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TCF12 aa 1-200.

View Alternative Names

BHLHB20, HEB, HTF4, TCF12, Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4, bHLHb20

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-TCF12 antibody (AB222040)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCF12 antibody (AB222040)

4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RH-30 cells stained for TCF12 (green) using ab222040 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TCF12 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q99081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCF12 also known as transcription factor 12 or Heb functions as a basic helix-loop-helix (bHLH) transcription factor. It plays roles in DNA binding and regulation of gene expression. The protein has a molecular mass of approximately 75 kDa. TCF12 expresses in various tissues with notable levels observed in the brain and skeletal muscle. It influences the development and differentiation of different cell types due to its transcriptional regulation activities.
Biological function summary

TCF12 controls the transcription of genes involved in cellular differentiation proliferation and development. It frequently functions as part of heterodimeric complexes with other bHLH proteins such as E2A. These complexes allow TCF12 to regulate a variety of target genes involved in tissue development and cell fate decisions. TCF12's ability to form these complexes enhances its functional diversity and impact on biological processes.

Pathways

The role of TCF12 extends to the Notch signaling pathway where it interacts with proteins like Notch receptors to affect cell fate determination. The protein also participates in the Myogenesis pathway collaborating with MyoD to facilitate the differentiation of myoblasts into mature muscle fibers. These pathways highlight TCF12's involvement in developmental processes and lineage specification.

TCF12 mutations and dysregulation have associations with craniosynostosis a condition characterized by premature fusion of cranial sutures. TCF12 also relates to certain types of cancer due to its regulatory effects on cell proliferation. In these contexts TCF12 may interact with proteins like TWIST1 which also influences developmental and tumorigenic processes highlighting potential therapeutic targets for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (By similarity). May be involved in the functional network that regulates the development of the GnRH axis (PubMed : 32620954).
See full target information TCF12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JACC. Basic to translational science 9:792-807 PubMed39070274

2024

Myocardial Infarction Suppresses Protein Synthesis and Causes Decoupling of Transcription and Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Shijie Liu,Vaibhav Deshmukh,Fangfei Wang,Jie Liang,Jenna Cusick,Xiao Li,James F Martin
View all publications
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Product promise

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