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AB69999

Anti-TCF3 / E2A antibody

3

(2 Reviews)

|

(9 Publications)

Rabbit Polyclonal TCF3 / E2A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human TCF3.

View Alternative Names

BHLHB21, E2A, ITF1, TCF3, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, Transcription factor ITF-1, bHLHb21, TCF-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody (AB69999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody (AB69999)

ab69999, at a 1/50 dilution, staining human TCF3/E2A in brain, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).

Western blot - Anti-TCF3 / E2A antibody (AB69999)
  • WB

Unknown

Western blot - Anti-TCF3 / E2A antibody (AB69999)

All lanes:

Western blot - Anti-TCF3 / E2A antibody (ab69999) at 1/500 dilution

Lane 1:

Extracts from 293 cells, minus immunising peptide at 5 µg

Lane 2:

Extracts from 293 cells, plus immunising peptide 5µg at 5 µg

Predicted band size: 67 kDa

Observed band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TCF3. The exact immunogen used to generate this antibody is proprietary information.

P15923

Specificity

Detects endogenous levels of total TCF3/E2A protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography, usingepitope specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCF3 protein also commonly known as E2A functions mechanistically as a transcription factor involved in the regulation of gene expression. It plays an important role in the initiation of transcription by binding to E-box sequences on DNA impacting the transcription of various target genes. The E2A protein has a molecular mass of approximately 68 kDa. You can find E2A expression in multiple tissues particularly within the lymphoid tissues where it is critical for lymphocyte development and function.
Biological function summary

TCF3/E2A is essential for proper cellular development and differentiation. It functions as part of a larger transcription complex and partners often with other transcription factors like HEB or E47. These interactions allow E2A to regulate diverse biological processes including B cell lineage commitment and neuronal development. The ability to influence these processes highlights E2A’s role in managing cellular fate and developmental outcomes.

Pathways

E2A participates actively in the Notch signaling and B-cell receptor signaling pathways. In the context of Notch signaling E2A interacts with proteins like NOTCH1 and RBPJ to influence cell lineage decisions. Meanwhile in the B-cell receptor signaling pathway E2A works closely with proteins such as PAX5 and EBF1 playing a part in B-lymphocyte proliferation and maturation. These pathways underline E2A's significant role in immune cell regulation and function.

E2A's activity holds importance in the context of lymphoblastic leukemia and neurodevelopmental disorders. Chromosomal translocations involving E2A such as the t(1;19) translocation associate with the development of acute lymphoblastic leukemia. These genetic changes can result in aberrant fusion proteins that disturb normal transcriptional regulation. E2A’s link to neurodevelopmental disorders emerges when its regulation becomes disrupted often involving proteins like NEUROD1 which partners with E2A in neural differentiation. This connection emphasizes E2A’s role in maintaining normal cellular and tissue functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity). Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity). Together with TCF15, required for the mesenchymal to epithelial transition (By similarity). Dimers bind DNA on E-box motifs : 5'-CANNTG-3' (By similarity). Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed : 2493990). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity).. Isoform E47. Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.
See full target information TCF3
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:110527 PubMed40977645

2025

Transcription factor 3 enhances hepatocellular carcinoma metastasis by upregulating matrix metalloproteinase-11.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Peng Tian,Tian-Hao Wu,Guang-Jun Zhang,Sheng-Jie Li

Oncology research 32:463-476 PubMed38370338

2024

MicroRNA-329-3p inhibits the Wnt/β-catenin pathway and proliferation of osteosarcoma cells by targeting transcription factor 7-like 1.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Sun,Masanori Kawano,Tatsuya Iwasaki,Ichiro Itonaga,Yuta Kubota,Hiroshi Tsumura,Kazuhiro Tanaka

Molecular cancer 20:123 PubMed34579723

2021

p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yang,Anpei Hu,Yanhua Guo,Jianqun Wang,Dan Li,Xiaojing Wang,Shikai Jin,Boling Yuan,Shuang Cai,Yi Zhou,Qilan Li,Guo Chen,Haiyang Gao,Liduan Zheng,Qiangsong Tong

The Journal of clinical endocrinology and metabolism 105: PubMed32119078

2020

Interleukin-6 (IL-6) Activates the NOTCH1 Signaling Pathway Through E-Proteins in Endometriotic Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Song,Ren-Wei Su,Niraj R Joshi,Tae Hoon Kim,Bruce A Lessey,Jae-Wook Jeong,Asgerally T Fazleabas

Journal of cellular physiology 234:11900-11911 PubMed30584670

2018

Parathyroid hormone-related peptide (1-34) promotes tooth eruption and inhibits osteogenesis of dental follicle cells during tooth development.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Zhang,Lijun Liao,Yuyu Li,Yang Xu,Weihua Guo,Weidong Tian,Shujuan Zou

Molecular therapy : the journal of the American So 25:2502-2512 PubMed28803863

2017

Epidermal E-Cadherin Dependent β-Catenin Pathway Is Phytochemical Inducible and Accelerates Anagen Hair Cycling.

Applications

Unspecified application

Species

Unspecified reactive species

Noha S Ahmed,Subhadip Ghatak,Mohamed S El Masry,Surya C Gnyawali,Sashwati Roy,Mohamed Amer,Helen Everts,Chandan K Sen,Savita Khanna

Oncotarget 6:32955-65 PubMed26460615

2015

Deciphering the molecular basis of invasiveness in Sdhb-deficient cells.

Applications

WB

Species

Unspecified reactive species

Céline Loriot,Mélanie Domingues,Adeline Berger,Mélanie Menara,Maëva Ruel,Aurélie Morin,Luis-Jaime Castro-Vega,Éric Letouzé,Cosimo Martinelli,Alexis-Pierre Bemelmans,Lionel Larue,Anne-Paule Gimenez-Roqueplo,Judith Favier

PloS one 9:e112005 PubMed25375219

2014

Hypomethylation-associated up-regulation of TCF3 expression and recurrence in stage II and III colorectal cancer.

Applications

IHC

Species

Human

Chunxiang Li,Sanjun Cai,Xishan Wang,Zheng Jiang

The EMBO journal 30:2662-74 PubMed21610693

2011

Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis.

Applications

IF, WB

Species

Mouse, Mouse

Kangae Lee,Nikolce Gjorevski,Eline Boghaert,Derek C Radisky,Celeste M Nelson
View all publications
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