AB224638

Anti-TCF3 / E2A antibody [EPR20682]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal TCF3 / E2A antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

BHLHB21, E2A, ITF1, TCF3, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, Transcription factor ITF-1, bHLHb21, TCF-3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [EPR20682] (AB224638)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [EPR20682] (AB224638)

Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling TCF3 / E2A with ab224638 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on tumor cells of human diffuse large B-cell lymphoma (PMID : 16877363). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [EPR20682] (AB224638)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling TCF3 / E2A with ab224638 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong nuclear staining on germinal center of human tonsil (PMID : 9665477). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [EPR20682] (AB224638)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling TCF3 / E2A with ab224638 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on tumor cells of human colon cancer (PMID : 24454819). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Carrier free

Anti-TCF3 / E2A antibody [EPR20682] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20682

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCF3 protein also commonly known as E2A functions mechanistically as a transcription factor involved in the regulation of gene expression. It plays an important role in the initiation of transcription by binding to E-box sequences on DNA impacting the transcription of various target genes. The E2A protein has a molecular mass of approximately 68 kDa. You can find E2A expression in multiple tissues particularly within the lymphoid tissues where it is critical for lymphocyte development and function.

Biological function summary

TCF3/E2A is essential for proper cellular development and differentiation. It functions as part of a larger transcription complex and partners often with other transcription factors like HEB or E47. These interactions allow E2A to regulate diverse biological processes including B cell lineage commitment and neuronal development. The ability to influence these processes highlights E2A’s role in managing cellular fate and developmental outcomes.

Pathways

E2A participates actively in the Notch signaling and B-cell receptor signaling pathways. In the context of Notch signaling E2A interacts with proteins like NOTCH1 and RBPJ to influence cell lineage decisions. Meanwhile in the B-cell receptor signaling pathway E2A works closely with proteins such as PAX5 and EBF1 playing a part in B-lymphocyte proliferation and maturation. These pathways underline E2A's significant role in immune cell regulation and function.

E2A's activity holds importance in the context of lymphoblastic leukemia and neurodevelopmental disorders. Chromosomal translocations involving E2A such as the t(1;19) translocation associate with the development of acute lymphoblastic leukemia. These genetic changes can result in aberrant fusion proteins that disturb normal transcriptional regulation. E2A’s link to neurodevelopmental disorders emerges when its regulation becomes disrupted often involving proteins like NEUROD1 which partners with E2A in neural differentiation. This connection emphasizes E2A’s role in maintaining normal cellular and tissue functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity). Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity). Together with TCF15, required for the mesenchymal to epithelial transition (By similarity). Dimers bind DNA on E-box motifs : 5'-CANNTG-3' (By similarity). Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed : 2493990). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity).. Isoform E47. Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.

See full target information TCF3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:1940-1950 PubMed40887500

2025

Mitochondrial dysfunction by glyoxalase 1 deficiency disrupts definitive endoderm and alveolar development of human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Suji Jeong,Hyebin Koh,Minje Kang,Ji-Young Kim,Roya Rasaei,Woo Jin Kim,Seon-Sook Han,In Sun Hong,Se-Ran Yang,Jong-Hee Lee,Seok-Ho Hong

Cell death discovery 11:303 PubMed40610429

2025

Transcription factor TCF3 promotes bladder cancer development via TMBIM6-Ca-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Feng Yang,Wei-Ming Guo,Qing-Tian Luo,Jingfen Lu,Zhou-Ke Tan,Yuan-Chun Ye,Gang Fan

Reproduction (Cambridge, England) 163:281-291 PubMed35239510

2022

TCF3 regulates human endometrial stromal cell proliferation and migration in RPL.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Wei Wei,Xue-Qing Liu,Yu-Chen Zhang,Chuan-Mei Qin,Yi Lin,Fu-Ju Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCF3 / E2A antibody [EPR20682]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [EPR20682] (AB224638)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [EPR20682] (AB224638)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [EPR20682] (AB224638)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise