AB316148

Anti-TCF3 / E2A antibody [EPR26691-38] - BSA and Azide free

Recombinant
RabMAb
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal TCF3 / E2A antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

Alf2, Me2, Tcfe2a, Tcf3, Transcription factor E2-alpha, Immunoglobulin enhancer-binding factor E12/E47, Transcription factor 3, Transcription factor A1, TCF-3

2 Images

Supplier Data

Western blot - Anti-TCF3 / E2A antibody [EPR26691-38] - BSA and Azide free (AB316148)

This data was developed using ab316147, the same antibody clone in a different buffer formulation.

Low expression : C2C12, NIH/3T3.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TCF3 / E2A antibody [EPR26691-38] (<a href='/en-us/products/primary-antibodies/tcf3-e2a-antibody-epr26691-38-ab316147'>ab316147</a>) at 1/1000 dilution

Lane 1:

WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 67 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-TCF3 / E2A antibody [EPR26691-38] - BSA and Azide free (AB316148)

This data was developed using ab316147, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle, hippocampus.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TCF3 / E2A antibody [EPR26691-38] (<a href='/en-us/products/primary-antibodies/tcf3-e2a-antibody-epr26691-38-ab316147'>ab316147</a>) at 1/1000 dilution

Lane 1:

Mouse breast cancer tissue lysate at 20 µg

Lane 2:

Mouse lymph node tissue lysate at 20 µg

Lane 3:

Mouse pancreas lysate at 20 µg

Lane 4:

Mouse skeletal muscle lysate at 20 µg

Lane 5:

Mouse hippocampus lysate at 20 µg

Lane 6:

Rat lymph node lysate at 20 µg

Lane 7:

Rat skeletal muscle lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 67 kDa,36 kDa

true

Exposure time: 81s

Unconjugated

Anti-TCF3 / E2A antibody [EPR26691-38]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26691-38

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

Product details

ab316148 is the carrirer-free version of ab316147.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCF3 protein also commonly known as E2A functions mechanistically as a transcription factor involved in the regulation of gene expression. It plays an important role in the initiation of transcription by binding to E-box sequences on DNA impacting the transcription of various target genes. The E2A protein has a molecular mass of approximately 68 kDa. You can find E2A expression in multiple tissues particularly within the lymphoid tissues where it is critical for lymphocyte development and function.

Biological function summary

TCF3/E2A is essential for proper cellular development and differentiation. It functions as part of a larger transcription complex and partners often with other transcription factors like HEB or E47. These interactions allow E2A to regulate diverse biological processes including B cell lineage commitment and neuronal development. The ability to influence these processes highlights E2A’s role in managing cellular fate and developmental outcomes.

Pathways

E2A participates actively in the Notch signaling and B-cell receptor signaling pathways. In the context of Notch signaling E2A interacts with proteins like NOTCH1 and RBPJ to influence cell lineage decisions. Meanwhile in the B-cell receptor signaling pathway E2A works closely with proteins such as PAX5 and EBF1 playing a part in B-lymphocyte proliferation and maturation. These pathways underline E2A's significant role in immune cell regulation and function.

E2A's activity holds importance in the context of lymphoblastic leukemia and neurodevelopmental disorders. Chromosomal translocations involving E2A such as the t(1;19) translocation associate with the development of acute lymphoblastic leukemia. These genetic changes can result in aberrant fusion proteins that disturb normal transcriptional regulation. E2A’s link to neurodevelopmental disorders emerges when its regulation becomes disrupted often involving proteins like NEUROD1 which partners with E2A in neural differentiation. This connection emphasizes E2A’s role in maintaining normal cellular and tissue functions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (PubMed : 15226298, PubMed : 18214987). Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (PubMed : 18214987). Together with TCF15, required for the mesenchymal to epithelial transition (PubMed : 11309385, PubMed : 15226298). Dimers bind DNA on E-box motifs : 5'-CANNTG-3' (PubMed : 15226298, PubMed : 18214987, PubMed : 30426815). Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (By similarity). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (PubMed : 2181401).. Isoform E47. Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.

See full target information Tcf3

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCF3 / E2A antibody [EPR26691-38] - BSA and Azide free

Western blot - Anti-TCF3 / E2A antibody [EPR26691-38] - BSA and Azide free (AB316148)

Western blot - Anti-TCF3 / E2A antibody [EPR26691-38] - BSA and Azide free (AB316148)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise