AB308211

Anti-TCF3 / E2A antibody [HL1954]

Recombinant
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Rabbit Recombinant Monoclonal TCF3 / E2A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TCF3.

View Alternative Names

BHLHB21, E2A, ITF1, TCF3, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, Transcription factor ITF-1, bHLHb21, TCF-3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed HeLa cells labelling TCF3 / E2A with ab308211 at 1/250 dilution at RT for 15 min.

Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000 dilution.

Supplier Data

Western blot - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

All lanes:

Western blot - Anti-TCF3 / E2A antibody [HL1954] (ab308211) at 1/1000 dilution

Lane 1:

NCI-H929 whole cell extract at 30 µg

Lane 2:

NCI-H929 nuclear extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67 kDa

false

Supplier Data

Western blot - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (ab308211) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Lane 1:

Jurkat whole cell extract at 30 µg

Lane 2:

Raji whole cell extract at 30 µg

Lane 3:

NCI-H929 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Supplier Data

Western blot - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

Immunofluorescent analysis of 4% paraformaldehyde-fixed SH-SY-5Y cells labeling TCF3 / E2A with ab308211 at 1/500 dilution. Green : TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (ab308211) diluted at 1/500. Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000. Blue : Fluoroshield with DAPI.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1954

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TCF3. The exact immunogen used to generate this antibody is proprietary information.

P15923

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCF3 protein also commonly known as E2A functions mechanistically as a transcription factor involved in the regulation of gene expression. It plays an important role in the initiation of transcription by binding to E-box sequences on DNA impacting the transcription of various target genes. The E2A protein has a molecular mass of approximately 68 kDa. You can find E2A expression in multiple tissues particularly within the lymphoid tissues where it is critical for lymphocyte development and function.

Biological function summary

TCF3/E2A is essential for proper cellular development and differentiation. It functions as part of a larger transcription complex and partners often with other transcription factors like HEB or E47. These interactions allow E2A to regulate diverse biological processes including B cell lineage commitment and neuronal development. The ability to influence these processes highlights E2A’s role in managing cellular fate and developmental outcomes.

Pathways

E2A participates actively in the Notch signaling and B-cell receptor signaling pathways. In the context of Notch signaling E2A interacts with proteins like NOTCH1 and RBPJ to influence cell lineage decisions. Meanwhile in the B-cell receptor signaling pathway E2A works closely with proteins such as PAX5 and EBF1 playing a part in B-lymphocyte proliferation and maturation. These pathways underline E2A's significant role in immune cell regulation and function.

E2A's activity holds importance in the context of lymphoblastic leukemia and neurodevelopmental disorders. Chromosomal translocations involving E2A such as the t(1;19) translocation associate with the development of acute lymphoblastic leukemia. These genetic changes can result in aberrant fusion proteins that disturb normal transcriptional regulation. E2A’s link to neurodevelopmental disorders emerges when its regulation becomes disrupted often involving proteins like NEUROD1 which partners with E2A in neural differentiation. This connection emphasizes E2A’s role in maintaining normal cellular and tissue functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity). Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity). Together with TCF15, required for the mesenchymal to epithelial transition (By similarity). Dimers bind DNA on E-box motifs : 5'-CANNTG-3' (By similarity). Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed : 2493990). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity).. Isoform E47. Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.

See full target information TCF3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCF3 / E2A antibody [HL1954]

Immunocytochemistry/ Immunofluorescence - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

Western blot - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

Western blot - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

Western blot - Anti-TCF3 / E2A antibody [HL1954] (AB308211)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise