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AB229605

Anti-TCF3 / E2A antibody - N-terminal

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(4 Publications)

Rabbit Polyclonal TCF3 / E2A antibody. N-terminal. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TCF3.

View Alternative Names

BHLHB21, E2A, ITF1, TCF3, Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, Transcription factor ITF-1, bHLHb21, TCF-3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TCF3 / E2A antibody - N-terminal (AB229605)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCF3 / E2A antibody - N-terminal (AB229605)

4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for TCF3 / E2A (green) using ab229605 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunoprecipitation - Anti-TCF3 / E2A antibody - N-terminal (AB229605)
  • IP

Supplier Data

Immunoprecipitation - Anti-TCF3 / E2A antibody - N-terminal (AB229605)

TCF3 / E2A was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 μg ab229605. Western blot was performed from the immunoprecipitate using ab229605 at 1/500 dilution.

Lane 1 : Jurkat whole cell extract 50 μg (Input).

Lane 2 : 4 μg of preimmune Rabbit IgG instead of ab229605 in Jurkat whole cell extract.

Lane 3 : ab229605 IP in Jurkat whole cell extract.

All lanes:

Immunoprecipitation - Anti-TCF3 / E2A antibody - N-terminal (ab229605)

Predicted band size: 67 kDa

true

Western blot - Anti-TCF3 / E2A antibody - N-terminal (AB229605)
  • WB

Supplier Data

Western blot - Anti-TCF3 / E2A antibody - N-terminal (AB229605)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-TCF3 / E2A antibody - N-terminal (ab229605) at 1/3000 dilution

Lane 1:

NCI-H929 whole cell extract at 30 µg

Lane 2:

NCI-H929 nuclear extract at 30 µg

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TCF3. The exact immunogen used to generate this antibody is proprietary information.

P15923

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCF3 protein also commonly known as E2A functions mechanistically as a transcription factor involved in the regulation of gene expression. It plays an important role in the initiation of transcription by binding to E-box sequences on DNA impacting the transcription of various target genes. The E2A protein has a molecular mass of approximately 68 kDa. You can find E2A expression in multiple tissues particularly within the lymphoid tissues where it is critical for lymphocyte development and function.
Biological function summary

TCF3/E2A is essential for proper cellular development and differentiation. It functions as part of a larger transcription complex and partners often with other transcription factors like HEB or E47. These interactions allow E2A to regulate diverse biological processes including B cell lineage commitment and neuronal development. The ability to influence these processes highlights E2A’s role in managing cellular fate and developmental outcomes.

Pathways

E2A participates actively in the Notch signaling and B-cell receptor signaling pathways. In the context of Notch signaling E2A interacts with proteins like NOTCH1 and RBPJ to influence cell lineage decisions. Meanwhile in the B-cell receptor signaling pathway E2A works closely with proteins such as PAX5 and EBF1 playing a part in B-lymphocyte proliferation and maturation. These pathways underline E2A's significant role in immune cell regulation and function.

E2A's activity holds importance in the context of lymphoblastic leukemia and neurodevelopmental disorders. Chromosomal translocations involving E2A such as the t(1;19) translocation associate with the development of acute lymphoblastic leukemia. These genetic changes can result in aberrant fusion proteins that disturb normal transcriptional regulation. E2A’s link to neurodevelopmental disorders emerges when its regulation becomes disrupted often involving proteins like NEUROD1 which partners with E2A in neural differentiation. This connection emphasizes E2A’s role in maintaining normal cellular and tissue functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity). Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity). Together with TCF15, required for the mesenchymal to epithelial transition (By similarity). Dimers bind DNA on E-box motifs : 5'-CANNTG-3' (By similarity). Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed : 2493990). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity).. Isoform E47. Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity.
See full target information TCF3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:110527 PubMed40977645

2025

Transcription factor 3 enhances hepatocellular carcinoma metastasis by upregulating matrix metalloproteinase-11.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Peng Tian,Tian-Hao Wu,Guang-Jun Zhang,Sheng-Jie Li

Bioengineered 12:7995-8005 PubMed34658308

2021

Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Dong,Jingjing Huang,Peng Zu,Jing Liu,Xue Gao,Jianwei Du,Yufu Li

The American journal of drug and alcohol abuse 47:581-589 PubMed34407719

2021

Differential expression and transcription factor binding associated with genotype at a pharmacogenetic variant in .

Applications

Unspecified application

Species

Unspecified reactive species

Richard C Crist,Gabriella Arauco-Shapiro,Alexander Zhang,Benjamin C Reiner,Wade H Berrettini,Glenn A Doyle

The Journal of nutritional biochemistry 80:108364 PubMed32199344

2020

Creb-Pgc1α pathway modulates the interaction between lipid droplets and mitochondria and influences high fat diet-induced changes of lipid metabolism in the liver and isolated hepatocytes of yellow catfish.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Feng Song,Christer Hogstrand,Shi-Cheng Ling,Guang-Hui Chen,Zhi Luo
View all publications
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