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AB327273

Anti-TCF7 Antibody

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Rabbit Polyclonal TCF7 antibody. Suitable for WB, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TCF7.

View Alternative Names

TCF1, TCF7, Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TCF7 Antibody (AB327273)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCF7 Antibody (AB327273)

Immunofluorescent staining of human cell line Hep G2 using ab327273 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP - Anti-TCF7 Antibody (AB327273)
  • ChIP

Supplier Data

ChIP - Anti-TCF7 Antibody (AB327273)

ChIP-Exo-Seq composite graph for ab327273 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-TCF7 Antibody (AB327273)
  • WB

Supplier Data

Western blot - Anti-TCF7 Antibody (AB327273)

Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma using ab327273 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-TCF7 Antibody (ab327273)

Lane 2:

Human cell line RT-4

Lane 3:

Human cell line U-251 MG

Lane 4:

Human plasma

Predicted band size: 41.55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ChIP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TCF7.

P36402

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.
See full target information TCF7

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