Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)

Rabbit Recombinant Monoclonal TCF7 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC/IF, mIHC, Flow Cyt (Intra) and reacts with Human samples.

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Images

Multiplex immunohistochemistry - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (AB315393), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IP	IHC-P	ICC/IF	mIHC	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information TCF7

Function

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.

Alternative names

TCF1, TCF7, Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1

Recommended products

Rabbit Recombinant Monoclonal TCF7 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC/IF, mIHC, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28579-5
Purification technique: Affinity purification Protein A
Specificity: This antibody should detect all TCF1/TCF7 isoforms, including those lacking the amino-terminal β-catenin binding domain.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab315393 is the carrier-free version of Anti-TCF7 antibody [EPR28579-5] ab315392.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TCF7 also known as TCF1 is a transcription factor belonging to the TCF/LEF family with a molecular mass of approximately 54 kDa. TCF7 plays a significant mechanical role in binding to DNA and regulating gene expression in cell nuclei. It interacts with beta-catenin to control the transcription of target genes. This protein is expressed in various tissues including the thymus and other parts of the immune system as well as the intestines.

Biological function summary

TCF7 regulates cell fate decisions and differentiation specifically in the T-cell lineage. It is part of a protein complex with beta-catenin and acts as a transcriptional activator. TCF7 influences the development and function of T-cells playing a critical role in adaptive immune response. It contributes to the maintenance of stem cell properties in various tissues impacting processes like cell proliferation and survival.

Pathways

TCF7 is a vital component of the Wnt/beta-catenin signaling pathway which is essential for regulating gene expression in various cellular processes. This pathway maintains homeostasis in cell proliferation differentiation and stem cell renewal. Within this signaling network beta-catenin an important mediator partners with TCF7 to modulate target gene transcription. TCF7 also interacts with other proteins such as LEF1 which similarly participates in the transcription regulation vital for cellular development.

Associated diseases and disorders

TCF7 associates with several pathological conditions including cancers and immunodeficiencies. Abnormal activation of the Wnt/beta-catenin pathway involving TCF7 contributes to the development of colon cancer by promoting unregulated cell division. TCF7 mutations have been linked to immunodeficiency disorders given its critical role in T-cell development. These mutations may disrupt normal T-cell formation leading to impaired immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Multiplex immunohistochemistry - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at a 1:2000 (0.26 ug/ml) dilution, Anti-CD3 epsilon antibody [CAL54] ab237707 anti-CD3E used at 1:500 (1.01 ug/ml) dilution and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 anti-CD19 used at a 1:5000 (0.21 ug/ml) dilution.
Panel A: merged staining of anti-TCF7 (magenta; Opal™690), anti-CD3E (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.

Panel B: anti-TCF7 staining T lymphocytes of human tonsil.

Panel C: anti-CD3E staining T lymphocytes of human tonsil.

Panel D: anti-CD19 staining B lymphocytes of human tonsil.
The section was incubated in three rounds of staining: in the order of Anti-TCF7 antibody [EPR28579-5] ab315392, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
This antibody should detect all TCF1/TCF7 isoforms, including those lacking the amino-terminal β-catenin binding domain.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-TCF7 antibody [EPR28579-5] (Anti-TCF7 antibody [EPR28579-5] ab315392) at 1/1000 dilution
Lane 1: Human thymus tissue lysate at 20 µg
Lane 2: Human tonsil tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 28-54 kDa, 36 kDa
Exposure time: 26s
Western blot - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
Low expression: LNCaP (PMID25436980).
This antibody should detect all TCF1/TCF7 isoforms, including those lacking the amino-terminal β-catenin binding domain.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-TCF7 antibody [EPR28579-5] (Anti-TCF7 antibody [EPR28579-5] ab315392) at 1/1000 dilution
Lane 1: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg
Lane 2: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 28-54 kDa, 36 kDa
Exposure time: 15s
Immunoprecipitation - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
TCF7 was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate with Anti-TCF7 antibody [EPR28579-5] ab315392 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TCF7 antibody [EPR28579-5] ab315392 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate

Lane 2: Anti-TCF7 antibody [EPR28579-5] ab315392 IP in MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TCF7 antibody [EPR28579-5] ab315392 in MOLT-4 whole cell lysate
All lanes: Immunoprecipitation - Anti-TCF7 antibody [EPR28579-5] (Anti-TCF7 antibody [EPR28579-5] ab315392) at 1/30 dilution
All lanes: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 32s
Flow Cytometry (Intracellular) - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized LNCaP (human prostate carcinoma epithelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: LNCaP.
Immunocytochemistry/ Immunofluorescence - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at 1/50 (9.8 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear staining in MOLT-4 cell line. Low expression: LNCaP (PMID: 25436980). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at 1/2000 (0.245 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human lung. The section was incubated with Anti-TCF7 antibody [EPR28579-5] ab315392 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at 1/2000 (0.245 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human skeletal muscle. The section was incubated with Anti-TCF7 antibody [EPR28579-5] ab315392 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's Lymphoma tissue labeling TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at 1/2000 (0.245 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human Hodgkin's Lymphoma. The section was incubated with Anti-TCF7 antibody [EPR28579-5] ab315392 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7 antibody [EPR28579-5] - BSA and Azide free (ab315393)
This data was developed using Anti-TCF7 antibody [EPR28579-5] ab315392, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at 1/2000 (0.245 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil (PMID: 12707037). The section was incubated with Anti-TCF7 antibody [EPR28579-5] ab315392 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.