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AB327135

Anti-TCF7L2 Antibody [CL11547]

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Mouse Monoclonal TCF-4/TCF7L2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TCF7L2.

View Alternative Names

TCF4, TCF7L2, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 Antibody [CL11547] (AB327135)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 Antibody [CL11547] (AB327135)

Immunohistochemical staining of human pancreas using ab327135 at 1 : 200–1 : 500 dilution, showing no nuclear positivity in exocrine glandular cells and islets of Langhernas.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 Antibody [CL11547] (AB327135)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 Antibody [CL11547] (AB327135)

Immunohistochemical staining of human lymph node using ab327135 at 1 : 200–1 : 500 dilution, showing very weak nuclear positivity in non-germinal center cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 Antibody [CL11547] (AB327135)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 Antibody [CL11547] (AB327135)

Immunohistochemical staining of human rectum using ab327135 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 Antibody [CL11547] (AB327135)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF7L2 Antibody [CL11547] (AB327135)

Immunohistochemical staining of mouse thalamus using ab327135 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in neuronal cells.

Western blot - Anti-TCF7L2 Antibody [CL11547] (AB327135)
  • WB

Supplier Data

Western blot - Anti-TCF7L2 Antibody [CL11547] (AB327135)

Western blot analysis in human cell line CAPAN-2 using ab327135 at 1 µg/ml.

All lanes:

Western blot - Anti-TCF7L2 Antibody [CL11547] (ab327135)

All lanes:

Human cell line CAPAN-2

Predicted band size: 67.92 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL11547

Isotype

IgG1

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TCF7L2.

Q9NQB0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
See full target information TCF7L2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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