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Rabbit Recombinant Monoclonal Tcl1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.

Alternative names

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Rabbit Recombinant Monoclonal Tcl1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR29392-92

Purification technique

Affinity purification Protein A

Specificity

This antibody does not cross-react with human MTCP1 and TCL1B.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab322331 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Tcl1. The recommended pair for sandwich ELISA is:

Capture: ab322330, Human Tcl1 Capture Antibody (unconjugated)

Detector: ab322331, Human Tcl1 Detector Antibody (unconjugated)

The reference range value is 1.95-125 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

Biological function summary

TCL1 enhances the activity of the AKT kinase an important component of cellular signaling that modulates cell growth and survival. This protein does not form any large complex but acts through AKT phosphorylation. The activation of TCL1 leads to the promotion of cell proliferation and prevention of apoptosis especially in lymphocytes. The regulation of these cellular activities by TCL1 suggests its important role in the development of the immune system.

Activity summary

The TCL1 (T-cell leukemia/lymphoma protein 1) also known as TCL1A is an important protein involved in cellular processes. TCL1 has a molecular mass of approximately 14 kDa and plays roles in signal transduction pathways. The protein is expressed in various tissues including the thymus lymph nodes and spleen. TCL1 is particularly important in T-cell development and function. It functions at a mechanistic level by interacting with other proteins thereby influencing cell signaling pathways essential for lymphocyte proliferation and survival.

Pathways

The AKT signaling pathway is significantly influenced by TCL1 controlling cell growth division and apoptosis. The interaction of TCL1 with AKT activates the PI3K/AKT pathway an important signal transduction pathway promoting survival and growth in response to extracellular signals. TCL1 indirectly affects the mTOR signaling pathway through its modulation of AKT affecting protein synthesis and cell metabolism. Both pathways emphasize the broad role of TCL1 in maintaining normal cellular functions and growth regulation.

Associated diseases and disorders

The overexpression or mutation of TCL1 can lead to malignancies such as T-cell leukemia and B-cell lymphoma. In these cancers abnormal TCL1 activity results in enhanced cell survival and proliferation contributing to tumorigenesis. In leukemia TCL1 directly interacts with AKT and its dysregulation becomes pathogenic. Research continues to investigate the involvement of TCL1 in oncogenesis and how its association with AKT and other signaling molecules like mTOR contributes to cancer progression. Understanding these interactions could potentially offer new therapeutic targets for treating related malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Anti-Tcl1 antibody [EPR29392-92] - BSA and Azide free (Detector) (ab322331), expandable thumbnail

    Sandwich ELISA - Anti-Tcl1 antibody [EPR29392-92] - BSA and Azide free (Detector) (ab322331)

    Sandwich ELISA of ab322329 with the capture antibody ab322330 dilution at 2 ug/mL and detector antibody ab322331 dilution at 0.5 ug/mL.

  • Sandwich ELISA - Anti-Tcl1 antibody [EPR29392-92] - BSA and Azide free (Detector) (ab322331), expandable thumbnail

    Sandwich ELISA - Anti-Tcl1 antibody [EPR29392-92] - BSA and Azide free (Detector) (ab322331)

    Interpolated concentration of native Tcl1 was measured at different sample concentrations. Undiluted sample is as follows: Ramos supernatant 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-Tcl1 antibody [EPR29392-92] - BSA and Azide free (Detector) (ab322331), expandable thumbnail

    Sandwich ELISA - Anti-Tcl1 antibody [EPR29392-92] - BSA and Azide free (Detector) (ab322331)

    Interpolated concentration of native Tcl1 was measured at different sample concentrations. Undiluted samples are as follows: human plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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