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Rabbit Recombinant Monoclonal Tcl1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [SP248] (AB225718), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Tcl1 antibody [SP248] (AB225718), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Tcl1 antibody [SP248] (AB225718), expandable thumbnail
  • Western blot - Anti-Tcl1 antibody [SP248] (AB225718), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [SP248] (AB225718), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/400

Notes

Primary incubation for 1 hour at room temperature.

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary incubation for 10 minutes at room temperature.

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/400

Notes

Primary incubation for 30 minutes at 4°C.

Associated Products

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Target data

Function

Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.

Alternative names

T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1, Protein p14 TCL1, Oncogene TCL1, TCL1, TCL1A

Recommended products

Rabbit Recombinant Monoclonal Tcl1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP248

Purification technique

Affinity purification Protein A

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [SP248] (ab225718), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [SP248] (ab225718)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Tcl1 with ab225718 at 1/100 dilution (1.32 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab225718 for 10 mins at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tcl1 antibody [SP248] (ab225718), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tcl1 antibody [SP248] (ab225718)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling Tcl1 with purified ab225718 at 1/50 (2.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-Tcl1 antibody [SP248] (ab225718), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Tcl1 antibody [SP248] (ab225718)

    Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling Tcl1 with purified ab225718 at 1/200 dilution (0.66μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Western blot - Anti-Tcl1 antibody [SP248] (ab225718), expandable thumbnail

    Western blot - Anti-Tcl1 antibody [SP248] (ab225718)

    All lanes: Western blot - Anti-Tcl1 antibody [SP248] (ab225718) at 1/400 dilution

    All lanes: Ramos (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [SP248] (ab225718), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [SP248] (ab225718)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Tcl1 using ab225718 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry (Intracellular) - Anti-Tcl1 antibody [SP248] (ab225718), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Tcl1 antibody [SP248] (ab225718)

    Intracellular flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells treated with ab225718 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com