Anti-Tcl1 antibody [SP248] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Tcl1 antibody [SP248] - BSA and Azide free (AB242426)

Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling Tcl1 with purified ab225718 at 1/200 dilution (0.66μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225718).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Tcl1 antibody [SP248] - BSA and Azide free (AB242426)

Intracellular flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells treated with ab225718 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab225718).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [SP248] - BSA and Azide free (AB242426)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Tcl1 using ab225718 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab225718).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [SP248] - BSA and Azide free (AB242426)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Tcl1 with ab225718 at 1/100 dilution (1.32 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab225718 for 10 mins at room temperature. This image was generated using ab225718, the same clone, but with a different buffer formulation.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tcl1 antibody [SP248] - BSA and Azide free (AB242426)

Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling Tcl1 with purified ab225718 at 1/50 (2.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225718).