Mouse Monoclonal Tcl1 antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TCL1A aa 1 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (AB268261), expandable thumbnail](https://content.abcam.com/products/images/tcl1-antibody-tcl1-2079-bsa-and-azide-free-ab268261--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10593.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (AB268261)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (AB268261), expandable thumbnail](https://content.abcam.com/products/images/tcl1-antibody-tcl1-2079-bsa-and-azide-free-ab268261--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57992.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (AB268261)")
![Protein Array - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (AB268261), expandable thumbnail](https://content.abcam.com/products/images/tcl1-antibody-tcl1-2079-bsa-and-azide-free-ab268261--protein-array-img141691.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (AB268261)")
![Western blot - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (AB268261), expandable thumbnail](https://content.abcam.com/products/images/tcl1-antibody-tcl1-2079-bsa-and-azide-free-ab268261--western-blot-img28354.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (AB268261)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human TCL1A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56279
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.
Alternative names
TCL1, TCL1A, T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1, Protein p14 TCL1, Oncogene TCL1
Recommended products
Mouse Monoclonal Tcl1 antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TCL1A aa 1 to C-terminus.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human TCL1A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56279
- Carrier free
- Yes
- Clone number
- TCL1/2079
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab268261 is the carrier-free version of Anti-Tcl1 antibody [TCL1/2079] ab268137.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TCL1 (T-cell leukemia/lymphoma protein 1) also known as TCL1A is an important protein involved in cellular processes. TCL1 has a molecular mass of approximately 14 kDa and plays roles in signal transduction pathways. The protein is expressed in various tissues including the thymus lymph nodes and spleen. TCL1 is particularly important in T-cell development and function. It functions at a mechanistic level by interacting with other proteins thereby influencing cell signaling pathways essential for lymphocyte proliferation and survival.
- Biological function summary
TCL1 enhances the activity of the AKT kinase an important component of cellular signaling that modulates cell growth and survival. This protein does not form any large complex but acts through AKT phosphorylation. The activation of TCL1 leads to the promotion of cell proliferation and prevention of apoptosis especially in lymphocytes. The regulation of these cellular activities by TCL1 suggests its important role in the development of the immune system.
- Pathways
The AKT signaling pathway is significantly influenced by TCL1 controlling cell growth division and apoptosis. The interaction of TCL1 with AKT activates the PI3K/AKT pathway an important signal transduction pathway promoting survival and growth in response to extracellular signals. TCL1 indirectly affects the mTOR signaling pathway through its modulation of AKT affecting protein synthesis and cell metabolism. Both pathways emphasize the broad role of TCL1 in maintaining normal cellular functions and growth regulation.
- Associated diseases and disorders
The overexpression or mutation of TCL1 can lead to malignancies such as T-cell leukemia and B-cell lymphoma. In these cancers abnormal TCL1 activity results in enhanced cell survival and proliferation contributing to tumorigenesis. In leukemia TCL1 directly interacts with AKT and its dysregulation becomes pathogenic. Research continues to investigate the involvement of TCL1 in oncogenesis and how its association with AKT and other signaling molecules like mTOR contributes to cancer progression. Understanding these interactions could potentially offer new therapeutic targets for treating related malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261), expandable thumbnail](https://content.abcam.com/products/images/tcl1-antibody-tcl1-2079-bsa-and-azide-free-ab268261--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10593.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261)
Formalin-fixed, paraffin-embedded human lymph node tissue stained with Anti-Tcl1 antibody [TCL1/2079] ab268137 at 2 μg/ml in immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium azide (Anti-Tcl1 antibody [TCL1/2079] ab268137).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261), expandable thumbnail](https://content.abcam.com/products/images/tcl1-antibody-tcl1-2079-bsa-and-azide-free-ab268261--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57992.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261)
Formalin-fixed, paraffin-embedded human tonsil tissue stained with Anti-Tcl1 antibody [TCL1/2079] ab268137 at 2 μg/ml in immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium azide (Anti-Tcl1 antibody [TCL1/2079] ab268137).
![Protein Array - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261), expandable thumbnail](https://content.abcam.com/products/images/tcl1-antibody-tcl1-2079-bsa-and-azide-free-ab268261--protein-array-img141691.jpg "Protein Array - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261)")
Protein Array - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261)
Protein Array containing more than 19,000 full-length human proteins using Anti-Tcl1 antibody [TCL1/2079] ab268137.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium azide (Anti-Tcl1 antibody [TCL1/2079] ab268137).
![Western blot - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261), expandable thumbnail](https://content.abcam.com/products/images/tcl1-antibody-tcl1-2079-bsa-and-azide-free-ab268261--western-blot-img28354.jpg "Western blot - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261)")
Western blot - Anti-Tcl1 antibody [TCL1/2079] - BSA and Azide free (ab268261)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium azide (Anti-Tcl1 antibody [TCL1/2079] ab268137).
All lanes: Western blot - Anti-Tcl1 antibody [TCL1/2079] (Anti-Tcl1 antibody [TCL1/2079] ab268137) at 2 µg/mL
All lanes: Raji (Human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 13 kDa
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