Anti-TCN1 antibody [EPR30835-583R]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCN1 antibody [EPR30835-583R] (AB325128)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TCN1 with ab325128 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : positive staining on neutrophils in human colon. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCN1 antibody [EPR30835-583R] (AB325128)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling TCN1 with ab325128 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on neutrophils in human spleen. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCN1 antibody [EPR30835-583R] (AB325128)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TCN1 with ab325128 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human breast carcinoma. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCN1 antibody [EPR30835-583R] (AB325128)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling TCN1 with ab325128 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung carcinoma. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCN1 antibody [EPR30835-583R] (AB325128)

Immunohistochemical analysis of paraffin-embedded Human salivary gland tissue labeling TCN1 with ab325128 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human salivary gland. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-TCN1 antibody [EPR30835-583R] (AB325128)

TCN1 was immunoprecipitated from 0.35 mg Human salivary gland tissue lysate with ab325128 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325128 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-TCN1 antibody [EPR30835-583R] (ab325128) at 1/1000 dilution

Lane 1:

Human salivary gland tissue lysate at 10 µg

Lane 2:

Human salivary gland tissue lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab325128 in human salivary gland tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/5000 dilution

Observed band size: 60-70 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-TCN1 antibody [EPR30835-583R] (AB325128)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : lymph node, spleen.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28638446; PMID : 32753569).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TCN1 antibody [EPR30835-583R] (ab325128) at 1/1000 dilution

Lane 1:

Untreated human salivary gland tissue lysate at 20 µg

Lane 2:

human salivary gland tissue lysate treated with Protein Deglycosylation MIX II at 20 µg

Lane 3:

Human lymph node tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Lane 5:

Human lung cancer tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60-70 kDa,36 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-TCN1 antibody [EPR30835-583R] (AB325128)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HEK-293, A549.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28638446; PMID : 32753569).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TCN1 antibody [EPR30835-583R] (ab325128) at 1/1000 dilution

Lane 1:

Untreated Calu-3 (human lung adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Calu-3 whole cell lysate treated with Protein Deglycosylation MIX II at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60-70 kDa,36 kDa

false

Exposure time: 180s