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AB65212

Anti-TCOF1 antibody

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(5 Publications)

Rabbit Polyclonal TCOF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TCOF1.

View Alternative Names

Treacle protein, Treacher Collins syndrome protein, TCOF1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (AB65212)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (AB65212)

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab65212 at a 1 : 50 dilution.
Left image untreated.
Right image treated with immunising peptide.

Western blot - Anti-TCOF1 antibody (AB65212)
  • WB

Unknown

Western blot - Anti-TCOF1 antibody (AB65212)

The amount of positive control loading for the WB is 5-30 ug of total protein.
The amount of the peptide for the WB is 5-10 ug.

All lanes:

Western blot - Anti-TCOF1 antibody (ab65212) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells

Lane 2:

Extracts from Jurkat cells, plus immunising peptide

Predicted band size: 152 kDa

Observed band size: 152 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TCOF1. The exact immunogen used to generate this antibody is proprietary information.

Q13428

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCOF1 also known as Treacle protein possesses a mechanical role in the nucleolus involved in ribosomal DNA transcription. This protein has a mass of approximately 153 kDa. It is expressed in a wide range of tissues but shows significant activity in neural crest cells during early development. TCOF1 helps in maintaining the integrity of ribosomal DNA and regulates the production of ribosomal RNA. Correct regulation of ribosomal RNA is essential for cellular function and survival.
Biological function summary

TCOF1 is critical in craniofacial development. The protein works as a part of the treacle complex interacting with RNA polymerase I and upstream binding factor 1 (UBF1) to facilitate ribosomal RNA synthesis. By supporting the transcriptional machinery TCOF1 impacts cellular proliferation and growth particularly in tissues derived from neural crest cells. This regulation of ribosomal RNA transcription links to various aspects of cellular and developmental biology.

Pathways

TCOF1 plays an important role in the ribosome biogenesis and p53 signaling pathways. In ribosome biogenesis TCOF1 interacts with proteins like NOLC1 to influence RNA polymerase I activity. The p53 signaling pathway involves TCOF1 as a ribosome biogenesis dysfunction can activate p53 which is an important player in the cellular checkpoint and apoptosis. This position in pathways hints at TCOF1's involvement in both regular growth processes and stress response mechanisms in cells.

TCOF1 mutations lead to Treacher Collins syndrome a condition resulting in craniofacial deformities. The disorder involves impaired development of facial bones due to disrupted ribosomal RNA transcription. Protein partners include POLR1C and POLR1D which also associate with Treacher Collins syndrome when mutated. TCOF1's role in RNA synthesis highlights its relevance in these developmental anomalies and provides insight into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed : 12777385, PubMed : 26399832). Required for neural crest specification : following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed : 26399832).
See full target information TCOF1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 19:e1010854 PubMed37639467

2023

rRNA transcription is integral to phase separation and maintenance of nucleolar structure.

Applications

Unspecified application

Species

Unspecified reactive species

Soma Dash,Maureen C Lamb,Jeffrey J Lange,Mary C McKinney,Dai Tsuchiya,Fengli Guo,Xia Zhao,Timothy J Corbin,MaryEllen Kirkman,Kym Delventhal,Emma L Moore,Sean McKinney,Rita Shiang,Paul A Trainor

Molecular genetics & genomic medicine 11:e2150 PubMed36808708

2023

Genetic mutations in ribosomal biogenesis gene TCOF1 identified in human neural tube defects.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wang,Haiqin Cheng,Qin Zhang,Jin Guo

The journal of histochemistry and cytochemistry : 64:669-686 PubMed27680669

2016

Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Franek,Alena Kovaříková,Eva Bártová,Stanislav Kozubek

Epigenetics & chromatin 7:39 PubMed25587355

2014

HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs.

Applications

ICC/IF

Species

Mouse

Lenka Stixová,Petra Sehnalová,Soňa Legartová,Jana Suchánková,Tereza Hrušková,Stanislav Kozubek,Dmitry V Sorokin,Pavel Matula,Ivan Raška,Aleš Kovařík,Jaroslav Fulneček,Eva Bártová

Human molecular genetics 19:3491-501 PubMed20591827

2010

CHD7 functions in the nucleolus as a positive regulator of ribosomal RNA biogenesis.

Applications

WB, ChIP

Species

Mouse, Mouse

Gabriel E Zentner,Elizabeth A Hurd,Michael P Schnetz,Lusy Handoko,Chuanping Wang,Zhenghe Wang,Chialin Wei,Paul J Tesar,Maria Hatzoglou,Donna M Martin,Peter C Scacheri
View all publications
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Product promise

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