Rabbit Polyclonal TCOF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TCOF1.
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- PLoS genetics 19:e10108542023rRNA transcription is integral to phase separation and maintenance of nucleolar structure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoma Dash,Maureen C Lamb,Jeffrey J Lange,Mary C McKinney,Dai Tsuchiya,Fengli Guo,Xia Zhao,Timothy J Corbin,MaryEllen Kirkman,Kym Delventhal,Emma L Moore,Sean McKinney,Rita Shiang,Paul A TrainorPubMed 37639467
- Molecular genetics & genomic medicine 11:e21502023Genetic mutations in ribosomal biogenesis gene TCOF1 identified in human neural tube defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Wang,Haiqin Cheng,Qin Zhang,Jin GuoPubMed 36808708
- The journal of histochemistry and cytochemistry : 64:669-6862016Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichal Franek et. al.PubMed 27680669
- Epigenetics & chromatin 7:392014HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs.
- Human molecular genetics 19:3491-5012010CHD7 functions in the nucleolus as a positive regulator of ribosomal RNA biogenesis.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TCOF1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13428
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
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Target data
Function
Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:12777385, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).
Alternative names
Treacle protein, Treacher Collins syndrome protein, TCOF1
Recommended products
Rabbit Polyclonal TCOF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TCOF1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TCOF1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13428
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCOF1 also known as Treacle protein possesses a mechanical role in the nucleolus involved in ribosomal DNA transcription. This protein has a mass of approximately 153 kDa. It is expressed in a wide range of tissues but shows significant activity in neural crest cells during early development. TCOF1 helps in maintaining the integrity of ribosomal DNA and regulates the production of ribosomal RNA. Correct regulation of ribosomal RNA is essential for cellular function and survival.
- Biological function summary
TCOF1 is critical in craniofacial development. The protein works as a part of the treacle complex interacting with RNA polymerase I and upstream binding factor 1 (UBF1) to facilitate ribosomal RNA synthesis. By supporting the transcriptional machinery TCOF1 impacts cellular proliferation and growth particularly in tissues derived from neural crest cells. This regulation of ribosomal RNA transcription links to various aspects of cellular and developmental biology.
- Pathways
TCOF1 plays an important role in the ribosome biogenesis and p53 signaling pathways. In ribosome biogenesis TCOF1 interacts with proteins like NOLC1 to influence RNA polymerase I activity. The p53 signaling pathway involves TCOF1 as a ribosome biogenesis dysfunction can activate p53 which is an important player in the cellular checkpoint and apoptosis. This position in pathways hints at TCOF1's involvement in both regular growth processes and stress response mechanisms in cells.
- Associated diseases and disorders
TCOF1 mutations lead to Treacher Collins syndrome a condition resulting in craniofacial deformities. The disorder involves impaired development of facial bones due to disrupted ribosomal RNA transcription. Protein partners include POLR1C and POLR1D which also associate with Treacher Collins syndrome when mutated. TCOF1's role in RNA synthesis highlights its relevance in these developmental anomalies and provides insight into potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-TCOF1 antibody (ab65212)
The amount of positive control loading for the WB is 5-30 ug of total protein.
The amount of the peptide for the WB is 5-10 ug.All lanes: Western blot - Anti-TCOF1 antibody (ab65212) at 1/500 dilution
Lane 1: Extracts from Jurkat cells
Lane 2: Extracts from Jurkat cells, plus immunising peptide
Predicted band size: 152 kDa
Observed band size: 152 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (ab65212)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab65212 at a 1:50 dilution.
Left image untreated.
Right image treated with immunising peptide.
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