AB240903

Anti-TCP1 alpha/CCTA antibody [91a]

Name: Anti-TCP1 alpha/CCTA antibody [91a] (ab240903) | Abcam
Brand: Abcam Limited
SKU: AB240903

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(2 Publications)

Rat Monoclonal TCP1 alpha/CCTA antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tcp1.

View Alternative Names

CCT1, CCTA, TCP1, T-complex protein 1 subunit alpha, TCP-1-alpha, CCT-alpha, Chaperonin containing T-complex polypeptide 1 subunit 1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (AB240903)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (AB240903)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (heat shocked for 1 hour at 42°)(human epithelial cell line from cervix adenocarcinoma) cells labeling TCP1 alpha/CCTA with ab240903 at 1/100 dilution for 12 hours at 4°C, followed by R-PE Goat Anti-Rat (yellow) at 1/200 dilution for 2 hours at room temperature (yellow). The nuclear counter stain is DAPI (blue).

A : DAPI, B : TCP1 alpha/CCTA, C : Composite.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (AB240903)

Immunofluorescent analysis of 2% paraformaldehyde-fixed HeLa (heat shocked for 1 hour at 42°) (human epithelial cell line from cervix adenocarcinoma) cells labeling TCP1 alpha/CCTA with ab240903 at 1/100 dilution for 12 hours at 4°C, followed by APC Goat Anti-Rat at 1/200 dilution for 2 hours at room temperature (red). The nuclear counter stain is DAPI (blue).

A : DAPI, B : TCP1 alpha/CCTA, C : Composite.

Supplier Data

Western blot - Anti-TCP1 alpha/CCTA antibody [91a] (AB240903)

All lanes:

Western blot - Anti-TCP1 alpha/CCTA antibody [91a] (ab240903) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 60 kDa

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Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

91a

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Tcp1. The exact immunogen used to generate this antibody is proprietary information.

P11983

Specificity

Detects ~60 & ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 alpha also known as CCTA or alpha TCP functions as a molecular chaperone within the cell. It assists in the folding of newly synthesized proteins specifically in large complexes. TCP1 alpha is one of eight subunits that form the chaperonin-containing TCP1 (CCT) complex weighing around 60 kDa. Researchers find TCP1 alpha in high concentrations in the cytoplasm expressing abundantly in tissues with high rates of protein synthesis such as testis and brain.

Biological function summary

The CCT complex which includes TCP1 alpha plays a significant role in maintaining cellular proteostasis by ensuring correct protein folding. It is essential in the folding of actin and tubulin both critical proteins for cytoskeletal functions. TCP1 alpha as part of the CCT complex prevents aggregation of polypeptides maintaining them in a functional state. By doing so TCP1 alpha influences numerous cellular processes including cell division and cytoskeletal arrangements.

Pathways

TCP1 alpha participates in essential cellular pathways such as the cytoskeleton organization and protein folding pathways. In cytoskeleton organization it ensures the correct folding of actin and tubulin which are important components for cytoskeletal integrity. This target also collaborates with heat shock proteins in protein folding pathways acting as a checkpoint to ensure only properly folded proteins advance through the pathway. Through these pathways TCP1 alpha keeps cellular structures and functions in check.

The malfunction of TCP1 alpha within the CCT complex has connections to neurodegenerative diseases and cancer. Aberrations in protein folding can lead to aggregations implicated in neurodegenerative disorders like Huntington's disease. Additionally the link between TCP1 alpha and imbalanced protein synthesis suggests its involvement in cancer cell proliferation where chaperone activity is upregulated. The interaction of TCP1 alpha with proteins like p53 a critical regulator in cancer highlights its role in disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed : 25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

See full target information TCP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 637:983-990 PubMed39663456

2024

In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor.

Applications

Unspecified application

Species

Unspecified reactive species

Huaipeng Xing,Remus R E Rosenkranz,Piere Rodriguez-Aliaga,Ting-Ting Lee,Tomáš Majtner,Stefanie Böhm,Beata Turoňová,Judith Frydman,Martin Beck

EXCLI journal 19:1141-1153 PubMed33013268

2020

LncRNA HOTAIR promotes MPP+-induced neuronal injury in Parkinson's disease by regulating the miR-874-5p/ATG10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingya Zhao,Hongli Li,Na Chang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TCP1 alpha/CCTA antibody [91a]

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (AB240903)

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 alpha/CCTA antibody [91a] (AB240903)

Western blot - Anti-TCP1 alpha/CCTA antibody [91a] (AB240903)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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